Transcriptome Profiling and Differential Gene Expression in Canine Microdissected Anagen and Telogen Hair Follicles and Interfollicular Epidermis
The transcriptome profile and differential gene expression in telogen and late anagen microdissected hair follicles and the interfollicular epidermis of healthy dogs was investigated by using RNAseq. The genes with the highest expression levels in each group were identified and genes known from stud...
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ftmdpi:oai:mdpi.com:/2073-4425/11/8/884/ 2023-08-20T04:05:47+02:00 Transcriptome Profiling and Differential Gene Expression in Canine Microdissected Anagen and Telogen Hair Follicles and Interfollicular Epidermis Dominique J. Wiener Kátia R. Groch Magdalena A.T. Brunner Tosso Leeb Vidhya Jagannathan Monika M. Welle agris 2020-08-04 application/pdf https://doi.org/10.3390/genes11080884 EN eng Multidisciplinary Digital Publishing Institute Animal Genetics and Genomics https://dx.doi.org/10.3390/genes11080884 https://creativecommons.org/licenses/by/4.0/ Genes; Volume 11; Issue 8; Pages: 884 dog Canis lupus familiaris dermatology hair cycle microdissection transcriptome analysis RNA-seq Text 2020 ftmdpi https://doi.org/10.3390/genes11080884 2023-07-31T23:52:40Z The transcriptome profile and differential gene expression in telogen and late anagen microdissected hair follicles and the interfollicular epidermis of healthy dogs was investigated by using RNAseq. The genes with the highest expression levels in each group were identified and genes known from studies in other species to be associated with structure and function of hair follicles and epidermis were evaluated. Transcriptome profiling revealed that late anagen follicles expressed mainly keratins and telogen follicles expressed GSN and KRT15. The interfollicular epidermis expressed predominately genes encoding for proteins associated with differentiation. All sample groups express genes encoding for proteins involved in cellular growth and signal transduction. The expression pattern of skin-associated genes in dogs is similar to humans. Differences in expression compared to mice and humans include BMP2 expression mainly in telogen and high KRT17 expression in the interfollicular epidermis of dogs. Our data provide the basis for the investigation of the structure and function of canine skin or skin disease and support the use of dogs as a model for human cutaneous disease by assigning gene expression to specific tissue states. Text Canis lupus MDPI Open Access Publishing Genes 11 8 884 |
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MDPI Open Access Publishing |
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ftmdpi |
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English |
topic |
dog Canis lupus familiaris dermatology hair cycle microdissection transcriptome analysis RNA-seq |
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dog Canis lupus familiaris dermatology hair cycle microdissection transcriptome analysis RNA-seq Dominique J. Wiener Kátia R. Groch Magdalena A.T. Brunner Tosso Leeb Vidhya Jagannathan Monika M. Welle Transcriptome Profiling and Differential Gene Expression in Canine Microdissected Anagen and Telogen Hair Follicles and Interfollicular Epidermis |
topic_facet |
dog Canis lupus familiaris dermatology hair cycle microdissection transcriptome analysis RNA-seq |
description |
The transcriptome profile and differential gene expression in telogen and late anagen microdissected hair follicles and the interfollicular epidermis of healthy dogs was investigated by using RNAseq. The genes with the highest expression levels in each group were identified and genes known from studies in other species to be associated with structure and function of hair follicles and epidermis were evaluated. Transcriptome profiling revealed that late anagen follicles expressed mainly keratins and telogen follicles expressed GSN and KRT15. The interfollicular epidermis expressed predominately genes encoding for proteins associated with differentiation. All sample groups express genes encoding for proteins involved in cellular growth and signal transduction. The expression pattern of skin-associated genes in dogs is similar to humans. Differences in expression compared to mice and humans include BMP2 expression mainly in telogen and high KRT17 expression in the interfollicular epidermis of dogs. Our data provide the basis for the investigation of the structure and function of canine skin or skin disease and support the use of dogs as a model for human cutaneous disease by assigning gene expression to specific tissue states. |
format |
Text |
author |
Dominique J. Wiener Kátia R. Groch Magdalena A.T. Brunner Tosso Leeb Vidhya Jagannathan Monika M. Welle |
author_facet |
Dominique J. Wiener Kátia R. Groch Magdalena A.T. Brunner Tosso Leeb Vidhya Jagannathan Monika M. Welle |
author_sort |
Dominique J. Wiener |
title |
Transcriptome Profiling and Differential Gene Expression in Canine Microdissected Anagen and Telogen Hair Follicles and Interfollicular Epidermis |
title_short |
Transcriptome Profiling and Differential Gene Expression in Canine Microdissected Anagen and Telogen Hair Follicles and Interfollicular Epidermis |
title_full |
Transcriptome Profiling and Differential Gene Expression in Canine Microdissected Anagen and Telogen Hair Follicles and Interfollicular Epidermis |
title_fullStr |
Transcriptome Profiling and Differential Gene Expression in Canine Microdissected Anagen and Telogen Hair Follicles and Interfollicular Epidermis |
title_full_unstemmed |
Transcriptome Profiling and Differential Gene Expression in Canine Microdissected Anagen and Telogen Hair Follicles and Interfollicular Epidermis |
title_sort |
transcriptome profiling and differential gene expression in canine microdissected anagen and telogen hair follicles and interfollicular epidermis |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/genes11080884 |
op_coverage |
agris |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Genes; Volume 11; Issue 8; Pages: 884 |
op_relation |
Animal Genetics and Genomics https://dx.doi.org/10.3390/genes11080884 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/genes11080884 |
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Genes |
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11 |
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8 |
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884 |
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1774716521894903808 |