Characterizing Gene Copy Number of Heat Shock Protein Gene Families in the Emerald Rockcod, Trematomus bernacchii
The suborder Notothenioidae is comprised of Antarctic fishes, several of which have lost their ability to rapidly upregulate heat shock proteins in response to thermal stress, instead adopting a pattern of expression resembling constitutive genes. Given the cold-denaturing effect that sub-zero water...
Published in: | Genes |
---|---|
Main Authors: | , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2020
|
Subjects: | |
Online Access: | https://doi.org/10.3390/genes11080867 |
id |
ftmdpi:oai:mdpi.com:/2073-4425/11/8/867/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/2073-4425/11/8/867/ 2023-08-20T04:00:51+02:00 Characterizing Gene Copy Number of Heat Shock Protein Gene Families in the Emerald Rockcod, Trematomus bernacchii Anthony D. Tercero Sean P. Place agris 2020-07-31 application/pdf https://doi.org/10.3390/genes11080867 EN eng Multidisciplinary Digital Publishing Institute Animal Genetics and Genomics https://dx.doi.org/10.3390/genes11080867 https://creativecommons.org/licenses/by/4.0/ Genes; Volume 11; Issue 8; Pages: 867 notothenioid heat shock proteins cellular stress molecular chaperones gene duplication Text 2020 ftmdpi https://doi.org/10.3390/genes11080867 2023-07-31T23:51:39Z The suborder Notothenioidae is comprised of Antarctic fishes, several of which have lost their ability to rapidly upregulate heat shock proteins in response to thermal stress, instead adopting a pattern of expression resembling constitutive genes. Given the cold-denaturing effect that sub-zero waters have on proteins, evolution in the Southern Ocean has likely selected for increased expression of molecular chaperones. These selective pressures may have also enabled retention of gene duplicates, bolstering quantitative output of cytosolic heat shock proteins (HSPs). Given that newly duplicated genes are under more relaxed selection, it is plausible that gene duplication enabled altered regulation of such highly conserved genes. To test for evidence of gene duplication, copy number of various isoforms within major heat shock gene families were characterized via qPCR and compared between the Antarctic notothen, Trematomus bernacchii, which lost the inducible heat shock response, and the non-Antarctic notothen, Notothenia angustata, which maintains an inducible heat shock response. The results indicate duplication of isoforms within the hsp70 and hsp40 super families have occurred in the genome of T. bernacchii. The findings suggest gene duplications may have been critical in maintaining protein folding efficiency in the sub-zero waters and provided an evolutionary mechanism of alternative regulation of these conserved gene families. Text Antarc* Antarctic Southern Ocean MDPI Open Access Publishing Antarctic Southern Ocean The Antarctic Genes 11 8 867 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
notothenioid heat shock proteins cellular stress molecular chaperones gene duplication |
spellingShingle |
notothenioid heat shock proteins cellular stress molecular chaperones gene duplication Anthony D. Tercero Sean P. Place Characterizing Gene Copy Number of Heat Shock Protein Gene Families in the Emerald Rockcod, Trematomus bernacchii |
topic_facet |
notothenioid heat shock proteins cellular stress molecular chaperones gene duplication |
description |
The suborder Notothenioidae is comprised of Antarctic fishes, several of which have lost their ability to rapidly upregulate heat shock proteins in response to thermal stress, instead adopting a pattern of expression resembling constitutive genes. Given the cold-denaturing effect that sub-zero waters have on proteins, evolution in the Southern Ocean has likely selected for increased expression of molecular chaperones. These selective pressures may have also enabled retention of gene duplicates, bolstering quantitative output of cytosolic heat shock proteins (HSPs). Given that newly duplicated genes are under more relaxed selection, it is plausible that gene duplication enabled altered regulation of such highly conserved genes. To test for evidence of gene duplication, copy number of various isoforms within major heat shock gene families were characterized via qPCR and compared between the Antarctic notothen, Trematomus bernacchii, which lost the inducible heat shock response, and the non-Antarctic notothen, Notothenia angustata, which maintains an inducible heat shock response. The results indicate duplication of isoforms within the hsp70 and hsp40 super families have occurred in the genome of T. bernacchii. The findings suggest gene duplications may have been critical in maintaining protein folding efficiency in the sub-zero waters and provided an evolutionary mechanism of alternative regulation of these conserved gene families. |
format |
Text |
author |
Anthony D. Tercero Sean P. Place |
author_facet |
Anthony D. Tercero Sean P. Place |
author_sort |
Anthony D. Tercero |
title |
Characterizing Gene Copy Number of Heat Shock Protein Gene Families in the Emerald Rockcod, Trematomus bernacchii |
title_short |
Characterizing Gene Copy Number of Heat Shock Protein Gene Families in the Emerald Rockcod, Trematomus bernacchii |
title_full |
Characterizing Gene Copy Number of Heat Shock Protein Gene Families in the Emerald Rockcod, Trematomus bernacchii |
title_fullStr |
Characterizing Gene Copy Number of Heat Shock Protein Gene Families in the Emerald Rockcod, Trematomus bernacchii |
title_full_unstemmed |
Characterizing Gene Copy Number of Heat Shock Protein Gene Families in the Emerald Rockcod, Trematomus bernacchii |
title_sort |
characterizing gene copy number of heat shock protein gene families in the emerald rockcod, trematomus bernacchii |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/genes11080867 |
op_coverage |
agris |
geographic |
Antarctic Southern Ocean The Antarctic |
geographic_facet |
Antarctic Southern Ocean The Antarctic |
genre |
Antarc* Antarctic Southern Ocean |
genre_facet |
Antarc* Antarctic Southern Ocean |
op_source |
Genes; Volume 11; Issue 8; Pages: 867 |
op_relation |
Animal Genetics and Genomics https://dx.doi.org/10.3390/genes11080867 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/genes11080867 |
container_title |
Genes |
container_volume |
11 |
container_issue |
8 |
container_start_page |
867 |
_version_ |
1774720800715177984 |