Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales
“Candidatus Micrarchaeota” are widely distributed in acidic environments; however, their cultivability and our understanding of their interactions with potential hosts are very limited. Their habitats were so far attributed with acidic sites, soils, peats, freshwater systems, and hypersaline mats. U...
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ftmdpi:oai:mdpi.com:/2073-4425/10/6/461/ 2023-08-20T04:07:40+02:00 Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales Olga V. Golyshina Rafael Bargiela Stepan V. Toshchakov Nikolay A. Chernyh Soshila Ramayah Aleksei A. Korzhenkov Ilya V. Kublanov Peter N. Golyshin agris 2019-06-15 application/pdf https://doi.org/10.3390/genes10060461 EN eng Multidisciplinary Digital Publishing Institute Microbial Genetics and Genomics https://dx.doi.org/10.3390/genes10060461 https://creativecommons.org/licenses/by/4.0/ Genes; Volume 10; Issue 6; Pages: 461 “ Ca . Mancarchaeum acidiphilum” ARMAN-2 “ Ca . Micrarchaeota” DPANN superphylum Thermoplasmatales Cuniculiplasma acidic environments acid mine drainage sites terrestrial hot springs Text 2019 ftmdpi https://doi.org/10.3390/genes10060461 2023-07-31T22:21:39Z “Candidatus Micrarchaeota” are widely distributed in acidic environments; however, their cultivability and our understanding of their interactions with potential hosts are very limited. Their habitats were so far attributed with acidic sites, soils, peats, freshwater systems, and hypersaline mats. Using cultivation and culture-independent approaches (16S rRNA gene clonal libraries, high-throughput amplicon sequencing of V3-V4 region of 16S rRNA genes), we surveyed the occurrence of these archaea in geothermal areas on Kamchatka Peninsula and Kunashir Island and assessed their taxonomic diversity in relation with another type of low-pH environment, acid mine drainage stream (Wales, UK). We detected “Ca. Micrarchaeota” in thermophilic heterotrophic enrichment cultures of Kunashir and Kamchatka that appeared as two different phylotypes, namely “Ca. Mancarchaeum acidiphilum”-, and ARMAN-2-related, alongside their potential hosts, Cuniculiplasma spp. and other Thermoplasmatales archaea without defined taxonomic position. These clusters of “Ca. Micrarchaeota” together with three other groups were also present in mesophilic acid mine drainage community. Present work expands our knowledge on the diversity of “Ca. Micrarchaeota” in thermophilic and mesophilic acidic environments, suggests cultivability patterns of acidophilic archaea and establishes potential links between low-abundance species of thermophilic “Ca. Micrarchaeota” and certain Thermoplasmatales, such as Cuniculiplasma spp. in situ. Text Kamchatka Kamchatka Peninsula MDPI Open Access Publishing Arman’ ENVELOPE(150.141,150.141,59.662,59.662) Kamchatka Peninsula ENVELOPE(160.000,160.000,56.000,56.000) Genes 10 6 461 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
“ Ca . Mancarchaeum acidiphilum” ARMAN-2 “ Ca . Micrarchaeota” DPANN superphylum Thermoplasmatales Cuniculiplasma acidic environments acid mine drainage sites terrestrial hot springs |
spellingShingle |
“ Ca . Mancarchaeum acidiphilum” ARMAN-2 “ Ca . Micrarchaeota” DPANN superphylum Thermoplasmatales Cuniculiplasma acidic environments acid mine drainage sites terrestrial hot springs Olga V. Golyshina Rafael Bargiela Stepan V. Toshchakov Nikolay A. Chernyh Soshila Ramayah Aleksei A. Korzhenkov Ilya V. Kublanov Peter N. Golyshin Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales |
topic_facet |
“ Ca . Mancarchaeum acidiphilum” ARMAN-2 “ Ca . Micrarchaeota” DPANN superphylum Thermoplasmatales Cuniculiplasma acidic environments acid mine drainage sites terrestrial hot springs |
description |
“Candidatus Micrarchaeota” are widely distributed in acidic environments; however, their cultivability and our understanding of their interactions with potential hosts are very limited. Their habitats were so far attributed with acidic sites, soils, peats, freshwater systems, and hypersaline mats. Using cultivation and culture-independent approaches (16S rRNA gene clonal libraries, high-throughput amplicon sequencing of V3-V4 region of 16S rRNA genes), we surveyed the occurrence of these archaea in geothermal areas on Kamchatka Peninsula and Kunashir Island and assessed their taxonomic diversity in relation with another type of low-pH environment, acid mine drainage stream (Wales, UK). We detected “Ca. Micrarchaeota” in thermophilic heterotrophic enrichment cultures of Kunashir and Kamchatka that appeared as two different phylotypes, namely “Ca. Mancarchaeum acidiphilum”-, and ARMAN-2-related, alongside their potential hosts, Cuniculiplasma spp. and other Thermoplasmatales archaea without defined taxonomic position. These clusters of “Ca. Micrarchaeota” together with three other groups were also present in mesophilic acid mine drainage community. Present work expands our knowledge on the diversity of “Ca. Micrarchaeota” in thermophilic and mesophilic acidic environments, suggests cultivability patterns of acidophilic archaea and establishes potential links between low-abundance species of thermophilic “Ca. Micrarchaeota” and certain Thermoplasmatales, such as Cuniculiplasma spp. in situ. |
format |
Text |
author |
Olga V. Golyshina Rafael Bargiela Stepan V. Toshchakov Nikolay A. Chernyh Soshila Ramayah Aleksei A. Korzhenkov Ilya V. Kublanov Peter N. Golyshin |
author_facet |
Olga V. Golyshina Rafael Bargiela Stepan V. Toshchakov Nikolay A. Chernyh Soshila Ramayah Aleksei A. Korzhenkov Ilya V. Kublanov Peter N. Golyshin |
author_sort |
Olga V. Golyshina |
title |
Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales |
title_short |
Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales |
title_full |
Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales |
title_fullStr |
Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales |
title_full_unstemmed |
Diversity of “Ca. Micrarchaeota” in Two Distinct Types of Acidic Environments and Their Associations with Thermoplasmatales |
title_sort |
diversity of “ca. micrarchaeota” in two distinct types of acidic environments and their associations with thermoplasmatales |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2019 |
url |
https://doi.org/10.3390/genes10060461 |
op_coverage |
agris |
long_lat |
ENVELOPE(150.141,150.141,59.662,59.662) ENVELOPE(160.000,160.000,56.000,56.000) |
geographic |
Arman’ Kamchatka Peninsula |
geographic_facet |
Arman’ Kamchatka Peninsula |
genre |
Kamchatka Kamchatka Peninsula |
genre_facet |
Kamchatka Kamchatka Peninsula |
op_source |
Genes; Volume 10; Issue 6; Pages: 461 |
op_relation |
Microbial Genetics and Genomics https://dx.doi.org/10.3390/genes10060461 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/genes10060461 |
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Genes |
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10 |
container_issue |
6 |
container_start_page |
461 |
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1774719466643390464 |