Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2)
The prevalence of nuclear cataracts was observed to be significantly higher among residents of tropical and subtropical regions compared to those of temperate and subarctic regions. We hypothesized that elevated environmental temperatures may pose a risk of nuclear cataract development. The results...
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ftmdpi:oai:mdpi.com:/2073-4409/9/12/2670/ 2023-08-20T04:10:03+02:00 Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2) Naoki Yamamoto Shun Takeda Natsuko Hatsusaka Noriko Hiramatsu Noriaki Nagai Saori Deguchi Yosuke Nakazawa Takumi Takata Sachiko Kodera Akimasa Hirata Eri Kubo Hiroshi Sasaki 2020-12-11 application/pdf https://doi.org/10.3390/cells9122670 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/cells9122670 https://creativecommons.org/licenses/by/4.0/ Cells; Volume 9; Issue 12; Pages: 2670 immortalized human lens epithelial cell clone NY2 iHLEC-NY2 low-temperature culture computer simulation environmental temperature crystalline lens temperature αA crystallin amyloid β Text 2020 ftmdpi https://doi.org/10.3390/cells9122670 2023-08-01T00:39:11Z The prevalence of nuclear cataracts was observed to be significantly higher among residents of tropical and subtropical regions compared to those of temperate and subarctic regions. We hypothesized that elevated environmental temperatures may pose a risk of nuclear cataract development. The results of our in silico simulation revealed that in temperate and tropical regions, the human lens temperature ranges from 35.0 °C to 37.5 °C depending on the environmental temperature. The medium temperature changes during the replacement regularly in the cell culture experiment were carefully monitored using a sensor connected to a thermometer and showed a decrease of 1.9 °C, 3.0 °C, 1.7 °C, and 0.1 °C, after 5 min when setting the temperature of the heat plate device at 35.0 °C, 37.5 °C, 40.0 °C, and 42.5 °C, respectively. In the newly created immortalized human lens epithelial cell line clone NY2 (iHLEC-NY2), the amounts of RNA synthesis of αA crystallin, protein expression, and amyloid β (Aβ)1-40 secreted into the medium were increased at the culture temperature of 37.5 °C compared to 35.0 °C. In short-term culture experiments, the secretion of Aβ1-40 observed in cataracts was increased at 37.5 °C compared to 35.0 °C, suggesting that the long-term exposure to a high-temperature environment may increase the risk of cataracts. Text Subarctic MDPI Open Access Publishing Cells 9 12 2670 |
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English |
topic |
immortalized human lens epithelial cell clone NY2 iHLEC-NY2 low-temperature culture computer simulation environmental temperature crystalline lens temperature αA crystallin amyloid β |
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immortalized human lens epithelial cell clone NY2 iHLEC-NY2 low-temperature culture computer simulation environmental temperature crystalline lens temperature αA crystallin amyloid β Naoki Yamamoto Shun Takeda Natsuko Hatsusaka Noriko Hiramatsu Noriaki Nagai Saori Deguchi Yosuke Nakazawa Takumi Takata Sachiko Kodera Akimasa Hirata Eri Kubo Hiroshi Sasaki Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2) |
topic_facet |
immortalized human lens epithelial cell clone NY2 iHLEC-NY2 low-temperature culture computer simulation environmental temperature crystalline lens temperature αA crystallin amyloid β |
description |
The prevalence of nuclear cataracts was observed to be significantly higher among residents of tropical and subtropical regions compared to those of temperate and subarctic regions. We hypothesized that elevated environmental temperatures may pose a risk of nuclear cataract development. The results of our in silico simulation revealed that in temperate and tropical regions, the human lens temperature ranges from 35.0 °C to 37.5 °C depending on the environmental temperature. The medium temperature changes during the replacement regularly in the cell culture experiment were carefully monitored using a sensor connected to a thermometer and showed a decrease of 1.9 °C, 3.0 °C, 1.7 °C, and 0.1 °C, after 5 min when setting the temperature of the heat plate device at 35.0 °C, 37.5 °C, 40.0 °C, and 42.5 °C, respectively. In the newly created immortalized human lens epithelial cell line clone NY2 (iHLEC-NY2), the amounts of RNA synthesis of αA crystallin, protein expression, and amyloid β (Aβ)1-40 secreted into the medium were increased at the culture temperature of 37.5 °C compared to 35.0 °C. In short-term culture experiments, the secretion of Aβ1-40 observed in cataracts was increased at 37.5 °C compared to 35.0 °C, suggesting that the long-term exposure to a high-temperature environment may increase the risk of cataracts. |
format |
Text |
author |
Naoki Yamamoto Shun Takeda Natsuko Hatsusaka Noriko Hiramatsu Noriaki Nagai Saori Deguchi Yosuke Nakazawa Takumi Takata Sachiko Kodera Akimasa Hirata Eri Kubo Hiroshi Sasaki |
author_facet |
Naoki Yamamoto Shun Takeda Natsuko Hatsusaka Noriko Hiramatsu Noriaki Nagai Saori Deguchi Yosuke Nakazawa Takumi Takata Sachiko Kodera Akimasa Hirata Eri Kubo Hiroshi Sasaki |
author_sort |
Naoki Yamamoto |
title |
Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2) |
title_short |
Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2) |
title_full |
Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2) |
title_fullStr |
Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2) |
title_full_unstemmed |
Effect of a Lens Protein in Low-Temperature Culture of Novel Immortalized Human Lens Epithelial Cells (iHLEC-NY2) |
title_sort |
effect of a lens protein in low-temperature culture of novel immortalized human lens epithelial cells (ihlec-ny2) |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/cells9122670 |
genre |
Subarctic |
genre_facet |
Subarctic |
op_source |
Cells; Volume 9; Issue 12; Pages: 2670 |
op_relation |
https://dx.doi.org/10.3390/cells9122670 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/cells9122670 |
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Cells |
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9 |
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12 |
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2670 |
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