Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies
The development of fish oral vaccines is of great interest to the aquaculture industry due to the possibility of rapid vaccination of a large number of animals at reduced cost. In a previous study, we evaluated the effect of alginate-encapsulated Piscirickettsia salmonis antigens (AEPSA) incorporate...
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2022
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ftmdpi:oai:mdpi.com:/2073-4360/14/23/5115/ 2023-08-20T04:05:20+02:00 Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies Daniela Sotomayor-Gerding José Miguel Troncoso Katherine Díaz-Riquelme Karin Mariana Torres-Obreque Juan Cumilaf Alejandro J. Yañez Mónica Rubilar 2022-11-24 application/pdf https://doi.org/10.3390/polym14235115 EN eng Multidisciplinary Digital Publishing Institute Polymer Applications https://dx.doi.org/10.3390/polym14235115 https://creativecommons.org/licenses/by/4.0/ Polymers; Volume 14; Issue 23; Pages: 5115 Piscirickettsia salmonis oral vaccine alginate fish feed vacuum coating particle size encapsulation efficiency antigen stability Micro-CT-scanning Text 2022 ftmdpi https://doi.org/10.3390/polym14235115 2023-08-01T07:29:42Z The development of fish oral vaccines is of great interest to the aquaculture industry due to the possibility of rapid vaccination of a large number of animals at reduced cost. In a previous study, we evaluated the effect of alginate-encapsulated Piscirickettsia salmonis antigens (AEPSA) incorporated in feed, effectively enhancing the immune response in Atlantic salmon (Salmo salar). In this study, we seek to characterize AEPSA produced by ionic gelation using an aerodynamically assisted jetting (AAJ) system, to optimize microencapsulation efficiency (EE%), to assess microparticle stability against environmental (pH, salinity and temperature) and gastrointestinal conditions, and to evaluate microparticle incorporation in fish feed pellets through micro-CT-scanning. The AAJ system was effective in obtaining small microparticles (d < 20 μm) with a high EE% (97.92%). Environmental conditions (pH, salinity and temperature) generated instability in the microparticles, triggering protein release. 62.42% of the protein content was delivered at the intestinal level after in vitro digestion. Finally, micro-CT-scanning images confirmed microparticle incorporation in fish feed pellets. In conclusion, the AAJ system is effective at encapsulating P. salmonis antigens in alginate with a high EE% and a size small enough to be incorporated in fish feed and produce an oral vaccine. Text Atlantic salmon Salmo salar MDPI Open Access Publishing Polymers 14 23 5115 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Piscirickettsia salmonis oral vaccine alginate fish feed vacuum coating particle size encapsulation efficiency antigen stability Micro-CT-scanning |
spellingShingle |
Piscirickettsia salmonis oral vaccine alginate fish feed vacuum coating particle size encapsulation efficiency antigen stability Micro-CT-scanning Daniela Sotomayor-Gerding José Miguel Troncoso Katherine Díaz-Riquelme Karin Mariana Torres-Obreque Juan Cumilaf Alejandro J. Yañez Mónica Rubilar Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies |
topic_facet |
Piscirickettsia salmonis oral vaccine alginate fish feed vacuum coating particle size encapsulation efficiency antigen stability Micro-CT-scanning |
description |
The development of fish oral vaccines is of great interest to the aquaculture industry due to the possibility of rapid vaccination of a large number of animals at reduced cost. In a previous study, we evaluated the effect of alginate-encapsulated Piscirickettsia salmonis antigens (AEPSA) incorporated in feed, effectively enhancing the immune response in Atlantic salmon (Salmo salar). In this study, we seek to characterize AEPSA produced by ionic gelation using an aerodynamically assisted jetting (AAJ) system, to optimize microencapsulation efficiency (EE%), to assess microparticle stability against environmental (pH, salinity and temperature) and gastrointestinal conditions, and to evaluate microparticle incorporation in fish feed pellets through micro-CT-scanning. The AAJ system was effective in obtaining small microparticles (d < 20 μm) with a high EE% (97.92%). Environmental conditions (pH, salinity and temperature) generated instability in the microparticles, triggering protein release. 62.42% of the protein content was delivered at the intestinal level after in vitro digestion. Finally, micro-CT-scanning images confirmed microparticle incorporation in fish feed pellets. In conclusion, the AAJ system is effective at encapsulating P. salmonis antigens in alginate with a high EE% and a size small enough to be incorporated in fish feed and produce an oral vaccine. |
format |
Text |
author |
Daniela Sotomayor-Gerding José Miguel Troncoso Katherine Díaz-Riquelme Karin Mariana Torres-Obreque Juan Cumilaf Alejandro J. Yañez Mónica Rubilar |
author_facet |
Daniela Sotomayor-Gerding José Miguel Troncoso Katherine Díaz-Riquelme Karin Mariana Torres-Obreque Juan Cumilaf Alejandro J. Yañez Mónica Rubilar |
author_sort |
Daniela Sotomayor-Gerding |
title |
Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies |
title_short |
Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies |
title_full |
Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies |
title_fullStr |
Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies |
title_full_unstemmed |
Microencapsulation of Piscirickettsia salmonis Antigens for Fish Oral Immunization: Optimization and Stability Studies |
title_sort |
microencapsulation of piscirickettsia salmonis antigens for fish oral immunization: optimization and stability studies |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/polym14235115 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Polymers; Volume 14; Issue 23; Pages: 5115 |
op_relation |
Polymer Applications https://dx.doi.org/10.3390/polym14235115 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/polym14235115 |
container_title |
Polymers |
container_volume |
14 |
container_issue |
23 |
container_start_page |
5115 |
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1774715825659314176 |