Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6
Arctic bacteria employ various mechanisms to survive harsh conditions, one of which is to accumulate carbon and energy inside the cell in the form of polyhydroxyalkanoate (PHA). Whole-genome sequencing of a new Arctic soil bacterium Pseudomonas sp. B14-6 revealed two PHA-production-related gene clus...
Published in: | Polymers |
---|---|
Main Authors: | , , , , , , , , , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2021
|
Subjects: | |
Online Access: | https://doi.org/10.3390/polym13091398 |
id |
ftmdpi:oai:mdpi.com:/2073-4360/13/9/1398/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/2073-4360/13/9/1398/ 2023-08-20T04:04:05+02:00 Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6 Tae-Rim Choi Ye-Lim Park Hun-Suk Song Sun Mi Lee Sol Lee Park Hye Soo Lee Hyun-Joong Kim Shashi Kant Bhatia Ranjit Gurav Kwon-Young Choi Yoo Kyung Lee Yung-Hun Yang 2021-04-26 application/pdf https://doi.org/10.3390/polym13091398 EN eng Multidisciplinary Digital Publishing Institute Biomacromolecules, Biobased and Biodegradable Polymers https://dx.doi.org/10.3390/polym13091398 https://creativecommons.org/licenses/by/4.0/ Polymers; Volume 13; Issue 9; Pages: 1398 Pseudomonas strain polyhydroxyalkanoate fructose syrup fermentation Text 2021 ftmdpi https://doi.org/10.3390/polym13091398 2023-08-01T01:35:20Z Arctic bacteria employ various mechanisms to survive harsh conditions, one of which is to accumulate carbon and energy inside the cell in the form of polyhydroxyalkanoate (PHA). Whole-genome sequencing of a new Arctic soil bacterium Pseudomonas sp. B14-6 revealed two PHA-production-related gene clusters containing four PHA synthase genes (phaC). Pseudomonas sp. B14-6 produced poly(6% 3-hydroxybutyrate-co-94% 3-hydroxyalkanoate) from various carbon sources, containing short-chain-length PHA (scl-PHA) and medium-chain-length PHA (mcl-PHA) composed of various monomers analyzed by GC-MS, such as 3-hydroxybutyrate, 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, 3-hydroxydodecenoic acid, 3-hydroxydodecanoic acid, and 3-hydroxytetradecanoic acid. By optimizing the PHA production media, we achieved 34.6% PHA content using 5% fructose, and 23.7% PHA content using 5% fructose syrup. Differential scanning calorimetry of the scl-co-mcl PHA determined a glass transition temperature (Tg) of 15.3 °C, melting temperature of 112.8 °C, crystallization temperature of 86.8 °C, and 3.82% crystallinity. In addition, gel permeation chromatography revealed a number average molecular weight of 3.6 × 104, weight average molecular weight of 9.1 × 104, and polydispersity index value of 2.5. Overall, the novel Pseudomonas sp. B14-6 produced a polymer with high medium-chain-length content, low Tg, and low crystallinity, indicating its potential use in medical applications. Text Arctic MDPI Open Access Publishing Arctic Polymers 13 9 1398 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Pseudomonas strain polyhydroxyalkanoate fructose syrup fermentation |
spellingShingle |
Pseudomonas strain polyhydroxyalkanoate fructose syrup fermentation Tae-Rim Choi Ye-Lim Park Hun-Suk Song Sun Mi Lee Sol Lee Park Hye Soo Lee Hyun-Joong Kim Shashi Kant Bhatia Ranjit Gurav Kwon-Young Choi Yoo Kyung Lee Yung-Hun Yang Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6 |
topic_facet |
Pseudomonas strain polyhydroxyalkanoate fructose syrup fermentation |
description |
Arctic bacteria employ various mechanisms to survive harsh conditions, one of which is to accumulate carbon and energy inside the cell in the form of polyhydroxyalkanoate (PHA). Whole-genome sequencing of a new Arctic soil bacterium Pseudomonas sp. B14-6 revealed two PHA-production-related gene clusters containing four PHA synthase genes (phaC). Pseudomonas sp. B14-6 produced poly(6% 3-hydroxybutyrate-co-94% 3-hydroxyalkanoate) from various carbon sources, containing short-chain-length PHA (scl-PHA) and medium-chain-length PHA (mcl-PHA) composed of various monomers analyzed by GC-MS, such as 3-hydroxybutyrate, 3-hydroxyhexanoate, 3-hydroxyoctanoate, 3-hydroxydecanoate, 3-hydroxydodecenoic acid, 3-hydroxydodecanoic acid, and 3-hydroxytetradecanoic acid. By optimizing the PHA production media, we achieved 34.6% PHA content using 5% fructose, and 23.7% PHA content using 5% fructose syrup. Differential scanning calorimetry of the scl-co-mcl PHA determined a glass transition temperature (Tg) of 15.3 °C, melting temperature of 112.8 °C, crystallization temperature of 86.8 °C, and 3.82% crystallinity. In addition, gel permeation chromatography revealed a number average molecular weight of 3.6 × 104, weight average molecular weight of 9.1 × 104, and polydispersity index value of 2.5. Overall, the novel Pseudomonas sp. B14-6 produced a polymer with high medium-chain-length content, low Tg, and low crystallinity, indicating its potential use in medical applications. |
format |
Text |
author |
Tae-Rim Choi Ye-Lim Park Hun-Suk Song Sun Mi Lee Sol Lee Park Hye Soo Lee Hyun-Joong Kim Shashi Kant Bhatia Ranjit Gurav Kwon-Young Choi Yoo Kyung Lee Yung-Hun Yang |
author_facet |
Tae-Rim Choi Ye-Lim Park Hun-Suk Song Sun Mi Lee Sol Lee Park Hye Soo Lee Hyun-Joong Kim Shashi Kant Bhatia Ranjit Gurav Kwon-Young Choi Yoo Kyung Lee Yung-Hun Yang |
author_sort |
Tae-Rim Choi |
title |
Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6 |
title_short |
Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6 |
title_full |
Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6 |
title_fullStr |
Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6 |
title_full_unstemmed |
Fructose-Based Production of Short-Chain-Length and Medium-Chain-Length Polyhydroxyalkanoate Copolymer by Arctic Pseudomonas sp. B14-6 |
title_sort |
fructose-based production of short-chain-length and medium-chain-length polyhydroxyalkanoate copolymer by arctic pseudomonas sp. b14-6 |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/polym13091398 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Polymers; Volume 13; Issue 9; Pages: 1398 |
op_relation |
Biomacromolecules, Biobased and Biodegradable Polymers https://dx.doi.org/10.3390/polym13091398 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/polym13091398 |
container_title |
Polymers |
container_volume |
13 |
container_issue |
9 |
container_start_page |
1398 |
_version_ |
1774714498817458176 |