Extraction Optimization, Purification, Antioxidant Activity, and Preliminary Structural Characterization of Crude Polysaccharide from an Arctic Chlorella sp.
The arctic strain of Chlorella sp. (Chlorella-Arc) exists in the coldest and driest arctic ecosystems, and it is a new resource of active polysaccharides. The extraction of crude polysaccharide from Chlorella-Arc was optimized using the response surface methodology. A crude polysaccharide yield of a...
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ftmdpi:oai:mdpi.com:/2073-4360/10/3/292/ 2023-08-20T04:03:39+02:00 Extraction Optimization, Purification, Antioxidant Activity, and Preliminary Structural Characterization of Crude Polysaccharide from an Arctic Chlorella sp. Hong Song Meilin He Chuankun Gu Dong Wei Yuqi Liang Junmei Yan Changhai Wang 2018-03-09 application/pdf https://doi.org/10.3390/polym10030292 EN eng Multidisciplinary Digital Publishing Institute Biomacromolecules, Biobased and Biodegradable Polymers https://dx.doi.org/10.3390/polym10030292 https://creativecommons.org/licenses/by/4.0/ Polymers; Volume 10; Issue 3; Pages: 292 arctic Chlorella sp. polysaccharide antioxidant activity preliminary structural characterization Text 2018 ftmdpi https://doi.org/10.3390/polym10030292 2023-07-31T21:25:25Z The arctic strain of Chlorella sp. (Chlorella-Arc) exists in the coldest and driest arctic ecosystems, and it is a new resource of active polysaccharides. The extraction of crude polysaccharide from Chlorella-Arc was optimized using the response surface methodology. A crude polysaccharide yield of approximately 9.62 ± 0.11% dry weight was obtained under these optimized conditions. Three fractions (P-I, P-II, and P-III) were present after purification by 2-diethylaminoethanol Sepharose Fast Flow and Sephadex G-100 chromatography. The P-IIa fraction demonstrated significant antioxidant activities. Moreover, P-IIa was an α- and β-type heteropolysaccharide with a pyran group and contained variable amounts of rhamnose, arabinose, glucose, and galactose based on fourier-transform infrared spectroscopy, high-performance liquid chromatography, and 1H and 13C nuclear magnetic resonance imaging. Production of high amounts of polysaccharide may allow further exploration of the microalgae Chlorella-Arc as a natural antioxidant. Text Arctic MDPI Open Access Publishing Arctic Polymers 10 3 292 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
arctic Chlorella sp. polysaccharide antioxidant activity preliminary structural characterization |
spellingShingle |
arctic Chlorella sp. polysaccharide antioxidant activity preliminary structural characterization Hong Song Meilin He Chuankun Gu Dong Wei Yuqi Liang Junmei Yan Changhai Wang Extraction Optimization, Purification, Antioxidant Activity, and Preliminary Structural Characterization of Crude Polysaccharide from an Arctic Chlorella sp. |
topic_facet |
arctic Chlorella sp. polysaccharide antioxidant activity preliminary structural characterization |
description |
The arctic strain of Chlorella sp. (Chlorella-Arc) exists in the coldest and driest arctic ecosystems, and it is a new resource of active polysaccharides. The extraction of crude polysaccharide from Chlorella-Arc was optimized using the response surface methodology. A crude polysaccharide yield of approximately 9.62 ± 0.11% dry weight was obtained under these optimized conditions. Three fractions (P-I, P-II, and P-III) were present after purification by 2-diethylaminoethanol Sepharose Fast Flow and Sephadex G-100 chromatography. The P-IIa fraction demonstrated significant antioxidant activities. Moreover, P-IIa was an α- and β-type heteropolysaccharide with a pyran group and contained variable amounts of rhamnose, arabinose, glucose, and galactose based on fourier-transform infrared spectroscopy, high-performance liquid chromatography, and 1H and 13C nuclear magnetic resonance imaging. Production of high amounts of polysaccharide may allow further exploration of the microalgae Chlorella-Arc as a natural antioxidant. |
format |
Text |
author |
Hong Song Meilin He Chuankun Gu Dong Wei Yuqi Liang Junmei Yan Changhai Wang |
author_facet |
Hong Song Meilin He Chuankun Gu Dong Wei Yuqi Liang Junmei Yan Changhai Wang |
author_sort |
Hong Song |
title |
Extraction Optimization, Purification, Antioxidant Activity, and Preliminary Structural Characterization of Crude Polysaccharide from an Arctic Chlorella sp. |
title_short |
Extraction Optimization, Purification, Antioxidant Activity, and Preliminary Structural Characterization of Crude Polysaccharide from an Arctic Chlorella sp. |
title_full |
Extraction Optimization, Purification, Antioxidant Activity, and Preliminary Structural Characterization of Crude Polysaccharide from an Arctic Chlorella sp. |
title_fullStr |
Extraction Optimization, Purification, Antioxidant Activity, and Preliminary Structural Characterization of Crude Polysaccharide from an Arctic Chlorella sp. |
title_full_unstemmed |
Extraction Optimization, Purification, Antioxidant Activity, and Preliminary Structural Characterization of Crude Polysaccharide from an Arctic Chlorella sp. |
title_sort |
extraction optimization, purification, antioxidant activity, and preliminary structural characterization of crude polysaccharide from an arctic chlorella sp. |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2018 |
url |
https://doi.org/10.3390/polym10030292 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Polymers; Volume 10; Issue 3; Pages: 292 |
op_relation |
Biomacromolecules, Biobased and Biodegradable Polymers https://dx.doi.org/10.3390/polym10030292 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/polym10030292 |
container_title |
Polymers |
container_volume |
10 |
container_issue |
3 |
container_start_page |
292 |
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1774714090477846528 |