Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors
The high cost and environmental impact of fossil-based organic carriers represent a critical bottleneck to their use in large-scale industrial processes. The present study demonstrates the applicability of rice husk as inexpensive renewable carrier for the immobilization of enzymes applicable sector...
Published in: | Catalysts |
---|---|
Main Authors: | , , , , , , , , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2018
|
Subjects: | |
Online Access: | https://doi.org/10.3390/catal8100471 |
id |
ftmdpi:oai:mdpi.com:/2073-4344/8/10/471/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/2073-4344/8/10/471/ 2023-08-20T04:00:18+02:00 Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors Marco Cespugli Simone Lotteria Luciano Navarini Valentina Lonzarich Lorenzo Del Terra Francesca Vita Marina Zweyer Giovanna Baldini Valerio Ferrario Cynthia Ebert Lucia Gardossi 2018-10-19 application/pdf https://doi.org/10.3390/catal8100471 EN eng Multidisciplinary Digital Publishing Institute Biocatalysis https://dx.doi.org/10.3390/catal8100471 https://creativecommons.org/licenses/by/4.0/ Catalysts; Volume 8; Issue 10; Pages: 471 covalent immobilization of enzymes rice husk polycondensation CaLB itaconic acid aspartase acrylamide renewable carriers biomass Text 2018 ftmdpi https://doi.org/10.3390/catal8100471 2023-07-31T21:47:23Z The high cost and environmental impact of fossil-based organic carriers represent a critical bottleneck to their use in large-scale industrial processes. The present study demonstrates the applicability of rice husk as inexpensive renewable carrier for the immobilization of enzymes applicable sectors where the covalent anchorage of the protein is a pre-requisite for preventing protein contamination while assuring the recyclability. Rice husk was oxidized and then functionalized with a di-amino spacer. The morphological characterization shed light on the properties that affect the functionalization processes. Lipase B from Candida antarctica (CaLB) and two commercial asparaginases were immobilized covalently achieving higher immobilization yield than previously reported. All enzymes were immobilized also on commercial epoxy methacrylic resins and the CaLB immobilized on rice husk demonstrated a higher efficiency in the solvent-free polycondensation of dimethylitaconate. CaLB on rice husk appears particularly suitable for applications in highly viscous processes because of the unusual combination of its low density and remarkable mechanical robustness. In the case of the two asparaginases, the biocatalyst immobilized on rice husk performed in aqueous solution at least as efficiently as the enzyme immobilized on methacrylic resins, although the rice husk loaded a lower amount of protein. Text Antarc* Antarctica MDPI Open Access Publishing Anchorage Catalysts 8 10 471 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
covalent immobilization of enzymes rice husk polycondensation CaLB itaconic acid aspartase acrylamide renewable carriers biomass |
spellingShingle |
covalent immobilization of enzymes rice husk polycondensation CaLB itaconic acid aspartase acrylamide renewable carriers biomass Marco Cespugli Simone Lotteria Luciano Navarini Valentina Lonzarich Lorenzo Del Terra Francesca Vita Marina Zweyer Giovanna Baldini Valerio Ferrario Cynthia Ebert Lucia Gardossi Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors |
topic_facet |
covalent immobilization of enzymes rice husk polycondensation CaLB itaconic acid aspartase acrylamide renewable carriers biomass |
description |
The high cost and environmental impact of fossil-based organic carriers represent a critical bottleneck to their use in large-scale industrial processes. The present study demonstrates the applicability of rice husk as inexpensive renewable carrier for the immobilization of enzymes applicable sectors where the covalent anchorage of the protein is a pre-requisite for preventing protein contamination while assuring the recyclability. Rice husk was oxidized and then functionalized with a di-amino spacer. The morphological characterization shed light on the properties that affect the functionalization processes. Lipase B from Candida antarctica (CaLB) and two commercial asparaginases were immobilized covalently achieving higher immobilization yield than previously reported. All enzymes were immobilized also on commercial epoxy methacrylic resins and the CaLB immobilized on rice husk demonstrated a higher efficiency in the solvent-free polycondensation of dimethylitaconate. CaLB on rice husk appears particularly suitable for applications in highly viscous processes because of the unusual combination of its low density and remarkable mechanical robustness. In the case of the two asparaginases, the biocatalyst immobilized on rice husk performed in aqueous solution at least as efficiently as the enzyme immobilized on methacrylic resins, although the rice husk loaded a lower amount of protein. |
format |
Text |
author |
Marco Cespugli Simone Lotteria Luciano Navarini Valentina Lonzarich Lorenzo Del Terra Francesca Vita Marina Zweyer Giovanna Baldini Valerio Ferrario Cynthia Ebert Lucia Gardossi |
author_facet |
Marco Cespugli Simone Lotteria Luciano Navarini Valentina Lonzarich Lorenzo Del Terra Francesca Vita Marina Zweyer Giovanna Baldini Valerio Ferrario Cynthia Ebert Lucia Gardossi |
author_sort |
Marco Cespugli |
title |
Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors |
title_short |
Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors |
title_full |
Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors |
title_fullStr |
Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors |
title_full_unstemmed |
Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors |
title_sort |
rice husk as an inexpensive renewable immobilization carrier for biocatalysts employed in the food, cosmetic and polymer sectors |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2018 |
url |
https://doi.org/10.3390/catal8100471 |
geographic |
Anchorage |
geographic_facet |
Anchorage |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Catalysts; Volume 8; Issue 10; Pages: 471 |
op_relation |
Biocatalysis https://dx.doi.org/10.3390/catal8100471 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/catal8100471 |
container_title |
Catalysts |
container_volume |
8 |
container_issue |
10 |
container_start_page |
471 |
_version_ |
1774717474207432704 |