Spongin-Based Scaffolds from Hippospongia communis Demosponge as an Effective Support for Lipase Immobilization
The main purpose of the study was to achieve effective immobilization of lipase B from Candida antarctica (CALB) onto 3D spongin-based scaffolds from Hippospongia communis marine demosponge for rapeseed oil transesterification. Successful immobilization onto the marine sponge skeleton was confirmed...
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ftmdpi:oai:mdpi.com:/2073-4344/7/5/147/ 2023-08-20T04:02:34+02:00 Spongin-Based Scaffolds from Hippospongia communis Demosponge as an Effective Support for Lipase Immobilization Jakub Zdarta Małgorzata Norman Wojciech Smułek Dariusz Moszyński Ewa Kaczorek Allison Stelling Hermann Ehrlich Teofil Jesionowski 2017-05-10 application/pdf https://doi.org/10.3390/catal7050147 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/catal7050147 https://creativecommons.org/licenses/by/4.0/ Catalysts; Volume 7; Issue 5; Pages: 147 Hippospongia communis scaffolds lipase B from Candida antarctica lipase immobilization immobilized lipase stability and reusability rapeseed oil transesterification Text 2017 ftmdpi https://doi.org/10.3390/catal7050147 2023-07-31T21:06:53Z The main purpose of the study was to achieve effective immobilization of lipase B from Candida antarctica (CALB) onto 3D spongin-based scaffolds from Hippospongia communis marine demosponge for rapeseed oil transesterification. Successful immobilization onto the marine sponge skeleton was confirmed for the first time. Lipase B-containing biocatalytic system exhibited the highest catalytic activity retention (89%) after 60 min of immobilization at pH 7 and temperature of 4 °C. Immobilization was found to improve the thermal and chemical stability compared to free lipase, and retain over 80% of its initial catalytic activity over a wide range of temperature (30–60 °C) and pH (6–9). Additionally, immobilized lipase has good storage stability and retains over 70% of its initial activity even after catalyzing of 25 reaction cycles. The obtained product was used in a transesterification reaction of rapeseed oil with methanol and proved to be an efficient biocatalyst for biofuel production. The highest conversion value and fatty acids methyl esters (FAME) concentration were observed after a process conducted at 40 °C and pH 10. The possible mechanism of interaction between the enzyme and the spongin-based support is proposed and discussed. Text Antarc* Antarctica MDPI Open Access Publishing Catalysts 7 5 147 |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
Hippospongia communis scaffolds lipase B from Candida antarctica lipase immobilization immobilized lipase stability and reusability rapeseed oil transesterification |
spellingShingle |
Hippospongia communis scaffolds lipase B from Candida antarctica lipase immobilization immobilized lipase stability and reusability rapeseed oil transesterification Jakub Zdarta Małgorzata Norman Wojciech Smułek Dariusz Moszyński Ewa Kaczorek Allison Stelling Hermann Ehrlich Teofil Jesionowski Spongin-Based Scaffolds from Hippospongia communis Demosponge as an Effective Support for Lipase Immobilization |
topic_facet |
Hippospongia communis scaffolds lipase B from Candida antarctica lipase immobilization immobilized lipase stability and reusability rapeseed oil transesterification |
description |
The main purpose of the study was to achieve effective immobilization of lipase B from Candida antarctica (CALB) onto 3D spongin-based scaffolds from Hippospongia communis marine demosponge for rapeseed oil transesterification. Successful immobilization onto the marine sponge skeleton was confirmed for the first time. Lipase B-containing biocatalytic system exhibited the highest catalytic activity retention (89%) after 60 min of immobilization at pH 7 and temperature of 4 °C. Immobilization was found to improve the thermal and chemical stability compared to free lipase, and retain over 80% of its initial catalytic activity over a wide range of temperature (30–60 °C) and pH (6–9). Additionally, immobilized lipase has good storage stability and retains over 70% of its initial activity even after catalyzing of 25 reaction cycles. The obtained product was used in a transesterification reaction of rapeseed oil with methanol and proved to be an efficient biocatalyst for biofuel production. The highest conversion value and fatty acids methyl esters (FAME) concentration were observed after a process conducted at 40 °C and pH 10. The possible mechanism of interaction between the enzyme and the spongin-based support is proposed and discussed. |
format |
Text |
author |
Jakub Zdarta Małgorzata Norman Wojciech Smułek Dariusz Moszyński Ewa Kaczorek Allison Stelling Hermann Ehrlich Teofil Jesionowski |
author_facet |
Jakub Zdarta Małgorzata Norman Wojciech Smułek Dariusz Moszyński Ewa Kaczorek Allison Stelling Hermann Ehrlich Teofil Jesionowski |
author_sort |
Jakub Zdarta |
title |
Spongin-Based Scaffolds from Hippospongia communis Demosponge as an Effective Support for Lipase Immobilization |
title_short |
Spongin-Based Scaffolds from Hippospongia communis Demosponge as an Effective Support for Lipase Immobilization |
title_full |
Spongin-Based Scaffolds from Hippospongia communis Demosponge as an Effective Support for Lipase Immobilization |
title_fullStr |
Spongin-Based Scaffolds from Hippospongia communis Demosponge as an Effective Support for Lipase Immobilization |
title_full_unstemmed |
Spongin-Based Scaffolds from Hippospongia communis Demosponge as an Effective Support for Lipase Immobilization |
title_sort |
spongin-based scaffolds from hippospongia communis demosponge as an effective support for lipase immobilization |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2017 |
url |
https://doi.org/10.3390/catal7050147 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Catalysts; Volume 7; Issue 5; Pages: 147 |
op_relation |
https://dx.doi.org/10.3390/catal7050147 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/catal7050147 |
container_title |
Catalysts |
container_volume |
7 |
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5 |
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147 |
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1774713090163605504 |