Climatic Chamber Stability Tests of Lipase-Catalytic Octyl-Sepharose Systems
The application of the climatic chamber presented in this paper to assess the storage stability of immobilized lipases is a new approach characterized by the potential of unifying the study conditions of biocatalysts created in various laboratories. The data achieved from storing lipases in the clim...
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ftmdpi:oai:mdpi.com:/2073-4344/13/3/501/ 2023-08-20T04:00:23+02:00 Climatic Chamber Stability Tests of Lipase-Catalytic Octyl-Sepharose Systems Tomasz Siódmiak Joanna Siódmiak Rafał Mastalerz Natalia Kocot Jacek Dulęba Gudmundur G. Haraldsson Dorota Wątróbska-Świetlikowska Michał Piotr Marszałł 2023-02-28 application/pdf https://doi.org/10.3390/catal13030501 EN eng Multidisciplinary Digital Publishing Institute Biocatalysis https://dx.doi.org/10.3390/catal13030501 https://creativecommons.org/licenses/by/4.0/ Catalysts; Volume 13; Issue 3; Pages: 501 lipase B from Candida antarctica lipase from Candida rugosa stability tests Octyl-Sepharose immobilization protocols Text 2023 ftmdpi https://doi.org/10.3390/catal13030501 2023-08-01T09:02:54Z The application of the climatic chamber presented in this paper to assess the storage stability of immobilized lipases is a new approach characterized by the potential of unifying the study conditions of biocatalysts created in various laboratories. The data achieved from storing lipases in the climatic chambers may be crucial for the chemical and pharmaceutical industry. Our paper describes the developed protocols for immobilization via interfacial activation of lipase B from Candida antarctica (CALB) and lipase OF from Candida rugosa (CRL-OF) on the Octyl-Sepharose CL-4B support. Optimization included buffers with different pH values of 4–9 and a wide range of ionic strength from 5 mM to 700 mM. It has been shown that the optimal medium for the CALB immobilization process on the tested support is a citrate buffer at pH 4 and high ionic strength of 500 mM. Implementing new optimal procedures enabled the hyperactivation of immobilized CALB (recovery activity 116.10 ± 1.70%) under the applicable reaction conditions using olive oil as a substrate. Importantly, CALB storage stability tests performed in a climatic chamber under drastic temperature and humidity conditions proved good stability of the developed biocatalyst (residual activity 218 ± 7.3% of dry form, after 7 days). At the same time, the low storage stability of CRL OF in a climatic chamber was demonstrated. It should be emphasized that the use of a climatic chamber to test the storage stability of a dry form of the studied lipases immobilized on Octyl-Sepharose CL-4B is, to our knowledge, described for the first time in the literature. Text Antarc* Antarctica MDPI Open Access Publishing Rugosa ENVELOPE(-61.250,-61.250,-62.633,-62.633) Catalysts 13 3 501 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
lipase B from Candida antarctica lipase from Candida rugosa stability tests Octyl-Sepharose immobilization protocols |
spellingShingle |
lipase B from Candida antarctica lipase from Candida rugosa stability tests Octyl-Sepharose immobilization protocols Tomasz Siódmiak Joanna Siódmiak Rafał Mastalerz Natalia Kocot Jacek Dulęba Gudmundur G. Haraldsson Dorota Wątróbska-Świetlikowska Michał Piotr Marszałł Climatic Chamber Stability Tests of Lipase-Catalytic Octyl-Sepharose Systems |
topic_facet |
lipase B from Candida antarctica lipase from Candida rugosa stability tests Octyl-Sepharose immobilization protocols |
description |
The application of the climatic chamber presented in this paper to assess the storage stability of immobilized lipases is a new approach characterized by the potential of unifying the study conditions of biocatalysts created in various laboratories. The data achieved from storing lipases in the climatic chambers may be crucial for the chemical and pharmaceutical industry. Our paper describes the developed protocols for immobilization via interfacial activation of lipase B from Candida antarctica (CALB) and lipase OF from Candida rugosa (CRL-OF) on the Octyl-Sepharose CL-4B support. Optimization included buffers with different pH values of 4–9 and a wide range of ionic strength from 5 mM to 700 mM. It has been shown that the optimal medium for the CALB immobilization process on the tested support is a citrate buffer at pH 4 and high ionic strength of 500 mM. Implementing new optimal procedures enabled the hyperactivation of immobilized CALB (recovery activity 116.10 ± 1.70%) under the applicable reaction conditions using olive oil as a substrate. Importantly, CALB storage stability tests performed in a climatic chamber under drastic temperature and humidity conditions proved good stability of the developed biocatalyst (residual activity 218 ± 7.3% of dry form, after 7 days). At the same time, the low storage stability of CRL OF in a climatic chamber was demonstrated. It should be emphasized that the use of a climatic chamber to test the storage stability of a dry form of the studied lipases immobilized on Octyl-Sepharose CL-4B is, to our knowledge, described for the first time in the literature. |
format |
Text |
author |
Tomasz Siódmiak Joanna Siódmiak Rafał Mastalerz Natalia Kocot Jacek Dulęba Gudmundur G. Haraldsson Dorota Wątróbska-Świetlikowska Michał Piotr Marszałł |
author_facet |
Tomasz Siódmiak Joanna Siódmiak Rafał Mastalerz Natalia Kocot Jacek Dulęba Gudmundur G. Haraldsson Dorota Wątróbska-Świetlikowska Michał Piotr Marszałł |
author_sort |
Tomasz Siódmiak |
title |
Climatic Chamber Stability Tests of Lipase-Catalytic Octyl-Sepharose Systems |
title_short |
Climatic Chamber Stability Tests of Lipase-Catalytic Octyl-Sepharose Systems |
title_full |
Climatic Chamber Stability Tests of Lipase-Catalytic Octyl-Sepharose Systems |
title_fullStr |
Climatic Chamber Stability Tests of Lipase-Catalytic Octyl-Sepharose Systems |
title_full_unstemmed |
Climatic Chamber Stability Tests of Lipase-Catalytic Octyl-Sepharose Systems |
title_sort |
climatic chamber stability tests of lipase-catalytic octyl-sepharose systems |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/catal13030501 |
long_lat |
ENVELOPE(-61.250,-61.250,-62.633,-62.633) |
geographic |
Rugosa |
geographic_facet |
Rugosa |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Catalysts; Volume 13; Issue 3; Pages: 501 |
op_relation |
Biocatalysis https://dx.doi.org/10.3390/catal13030501 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/catal13030501 |
container_title |
Catalysts |
container_volume |
13 |
container_issue |
3 |
container_start_page |
501 |
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1774717890367324160 |