High Internal Phase Pickering Emulsion Stabilized by Lipase-Coated ZIF-8 Nanoparticles towards Recyclable Biphasic Biocatalyst
High internal phase Pickering emulsion (Pickering HIPE) stabilized by enzyme-decorated metal-organic frameworks (MOFs) nanoparticles is developed for biphasic biocatalysts to enhance lipase catalysis and recycling. Specifically, enzyme decorated nanoparticles are prepared via ZIF-8 physisorption of...
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2023
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ftmdpi:oai:mdpi.com:/2073-4344/13/2/383/ 2023-08-20T04:02:20+02:00 High Internal Phase Pickering Emulsion Stabilized by Lipase-Coated ZIF-8 Nanoparticles towards Recyclable Biphasic Biocatalyst Chuanbang Xu Yan Sun Yuanyuan Sun Ruiyun Cai Shengmiao Zhang 2023-02-10 application/pdf https://doi.org/10.3390/catal13020383 EN eng Multidisciplinary Digital Publishing Institute Biocatalysis https://dx.doi.org/10.3390/catal13020383 https://creativecommons.org/licenses/by/4.0/ Catalysts; Volume 13; Issue 2; Pages: 383 enzyme decorated MOFs high internal phase Pickering emulsion interfacial biocatalysis Text 2023 ftmdpi https://doi.org/10.3390/catal13020383 2023-08-01T08:44:10Z High internal phase Pickering emulsion (Pickering HIPE) stabilized by enzyme-decorated metal-organic frameworks (MOFs) nanoparticles is developed for biphasic biocatalysts to enhance lipase catalysis and recycling. Specifically, enzyme decorated nanoparticles are prepared via ZIF-8 physisorption of a model lipase Candida antarctica Lipase B (CALB), named ZIF-8@CALB, to be both Pickering stabilizer and catalytic sites. An oil-in-water (o/w) Pickering HIPE with oil/water volume ratio of 3 could then be fabricated by homogenizing p-nitrophenyl palmitate (p-NPP) n-heptane solution into the ZIF-8@CALB aqueous dispersion. The biocatalytic hydrolysis of p-NPP is conducted by just standing the biphasic system at room temperature. The Pickering HIPE system achieves a product conversion of up to 48.9% within 0.5 h, whereas the p-NPP n-heptane solution system containing free CALB only achieves a stable product conversion of 6.8% for the same time. Moreover, the ZIF@CALB could be recovered by a simple centrifugation at 800 rpm, and then reused in the next cycle. The hydrolysis equilibrium conversion rate of p-NPP keeps over 40% for all 8 cycles, reflecting the high catalytic efficiency and recyclability of the Pickering HIPE. This study provides a new opportunity in designing Enzyme-MOFs-based Pickering interfacial biocatalyst for practical applications. Text Antarc* Antarctica MDPI Open Access Publishing Catalysts 13 2 383 |
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Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
enzyme decorated MOFs high internal phase Pickering emulsion interfacial biocatalysis |
spellingShingle |
enzyme decorated MOFs high internal phase Pickering emulsion interfacial biocatalysis Chuanbang Xu Yan Sun Yuanyuan Sun Ruiyun Cai Shengmiao Zhang High Internal Phase Pickering Emulsion Stabilized by Lipase-Coated ZIF-8 Nanoparticles towards Recyclable Biphasic Biocatalyst |
topic_facet |
enzyme decorated MOFs high internal phase Pickering emulsion interfacial biocatalysis |
description |
High internal phase Pickering emulsion (Pickering HIPE) stabilized by enzyme-decorated metal-organic frameworks (MOFs) nanoparticles is developed for biphasic biocatalysts to enhance lipase catalysis and recycling. Specifically, enzyme decorated nanoparticles are prepared via ZIF-8 physisorption of a model lipase Candida antarctica Lipase B (CALB), named ZIF-8@CALB, to be both Pickering stabilizer and catalytic sites. An oil-in-water (o/w) Pickering HIPE with oil/water volume ratio of 3 could then be fabricated by homogenizing p-nitrophenyl palmitate (p-NPP) n-heptane solution into the ZIF-8@CALB aqueous dispersion. The biocatalytic hydrolysis of p-NPP is conducted by just standing the biphasic system at room temperature. The Pickering HIPE system achieves a product conversion of up to 48.9% within 0.5 h, whereas the p-NPP n-heptane solution system containing free CALB only achieves a stable product conversion of 6.8% for the same time. Moreover, the ZIF@CALB could be recovered by a simple centrifugation at 800 rpm, and then reused in the next cycle. The hydrolysis equilibrium conversion rate of p-NPP keeps over 40% for all 8 cycles, reflecting the high catalytic efficiency and recyclability of the Pickering HIPE. This study provides a new opportunity in designing Enzyme-MOFs-based Pickering interfacial biocatalyst for practical applications. |
format |
Text |
author |
Chuanbang Xu Yan Sun Yuanyuan Sun Ruiyun Cai Shengmiao Zhang |
author_facet |
Chuanbang Xu Yan Sun Yuanyuan Sun Ruiyun Cai Shengmiao Zhang |
author_sort |
Chuanbang Xu |
title |
High Internal Phase Pickering Emulsion Stabilized by Lipase-Coated ZIF-8 Nanoparticles towards Recyclable Biphasic Biocatalyst |
title_short |
High Internal Phase Pickering Emulsion Stabilized by Lipase-Coated ZIF-8 Nanoparticles towards Recyclable Biphasic Biocatalyst |
title_full |
High Internal Phase Pickering Emulsion Stabilized by Lipase-Coated ZIF-8 Nanoparticles towards Recyclable Biphasic Biocatalyst |
title_fullStr |
High Internal Phase Pickering Emulsion Stabilized by Lipase-Coated ZIF-8 Nanoparticles towards Recyclable Biphasic Biocatalyst |
title_full_unstemmed |
High Internal Phase Pickering Emulsion Stabilized by Lipase-Coated ZIF-8 Nanoparticles towards Recyclable Biphasic Biocatalyst |
title_sort |
high internal phase pickering emulsion stabilized by lipase-coated zif-8 nanoparticles towards recyclable biphasic biocatalyst |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/catal13020383 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Catalysts; Volume 13; Issue 2; Pages: 383 |
op_relation |
Biocatalysis https://dx.doi.org/10.3390/catal13020383 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/catal13020383 |
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Catalysts |
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13 |
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2 |
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383 |
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1774712744811954176 |