Continuous Flow Glycolipid Synthesis Using a Packed Bed Reactor
Glycolipids are a class of biodegradable biosurfactants that are non-toxic and based on renewables, making them a sustainable alternative to petrochemical surfactants. Enzymatic synthesis allows a tailor-made production of these versatile compounds using sugar and fatty acid building blocks with rat...
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ftmdpi:oai:mdpi.com:/2073-4344/12/5/551/ 2023-08-20T04:01:50+02:00 Continuous Flow Glycolipid Synthesis Using a Packed Bed Reactor Rebecca Hollenbach Delphine Muller André Delavault Christoph Syldatk 2022-05-18 application/pdf https://doi.org/10.3390/catal12050551 EN eng Multidisciplinary Digital Publishing Institute Biocatalysis https://dx.doi.org/10.3390/catal12050551 https://creativecommons.org/licenses/by/4.0/ Catalysts; Volume 12; Issue 5; Pages: 551 continuous flow heterogenous biocatalysis packed bed reactor glycolipid biphasic system Candida antarctica Lipase B Text 2022 ftmdpi https://doi.org/10.3390/catal12050551 2023-08-01T05:05:02Z Glycolipids are a class of biodegradable biosurfactants that are non-toxic and based on renewables, making them a sustainable alternative to petrochemical surfactants. Enzymatic synthesis allows a tailor-made production of these versatile compounds using sugar and fatty acid building blocks with rationalized structures for targeted applications. Therefore, glycolipids can be comprehensively designed to outcompete conventional surfactants regarding their physicochemical properties. However, enzymatic glycolipid processes are struggling with both sugars and fatty acid solubilities in reaction media. Thus, continuous flow processes represent a powerful tool in designing efficient syntheses of sugar esters. In this study, a continuous enzymatic glycolipid production catalyzed by Novozyme 435® is presented as an unprecedented concept. A biphasic aqueous–organic system was investigated, allowing for the simultaneous solubilization of sugars and fatty acids. Owing to phase separation, the remaining non-acylated glucose was easily separated from the product stream and was refed to the reactor forming a closed-loop system. Productivity in the continuous process was higher compared to a batch one, with space–time yields of up to 1228 ± 65 µmol/L/h. A temperature of 70 °C resulted in the highest glucose-6-O-decanoate concentration in the Packed Bed Reactor (PBR). Consequently, the design of a continuous biocatalytic production is a step towards a more competitive glycolipid synthesis in the aim for industrialization. Text Antarc* Antarctica MDPI Open Access Publishing Catalysts 12 5 551 |
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Open Polar |
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MDPI Open Access Publishing |
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English |
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continuous flow heterogenous biocatalysis packed bed reactor glycolipid biphasic system Candida antarctica Lipase B |
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continuous flow heterogenous biocatalysis packed bed reactor glycolipid biphasic system Candida antarctica Lipase B Rebecca Hollenbach Delphine Muller André Delavault Christoph Syldatk Continuous Flow Glycolipid Synthesis Using a Packed Bed Reactor |
topic_facet |
continuous flow heterogenous biocatalysis packed bed reactor glycolipid biphasic system Candida antarctica Lipase B |
description |
Glycolipids are a class of biodegradable biosurfactants that are non-toxic and based on renewables, making them a sustainable alternative to petrochemical surfactants. Enzymatic synthesis allows a tailor-made production of these versatile compounds using sugar and fatty acid building blocks with rationalized structures for targeted applications. Therefore, glycolipids can be comprehensively designed to outcompete conventional surfactants regarding their physicochemical properties. However, enzymatic glycolipid processes are struggling with both sugars and fatty acid solubilities in reaction media. Thus, continuous flow processes represent a powerful tool in designing efficient syntheses of sugar esters. In this study, a continuous enzymatic glycolipid production catalyzed by Novozyme 435® is presented as an unprecedented concept. A biphasic aqueous–organic system was investigated, allowing for the simultaneous solubilization of sugars and fatty acids. Owing to phase separation, the remaining non-acylated glucose was easily separated from the product stream and was refed to the reactor forming a closed-loop system. Productivity in the continuous process was higher compared to a batch one, with space–time yields of up to 1228 ± 65 µmol/L/h. A temperature of 70 °C resulted in the highest glucose-6-O-decanoate concentration in the Packed Bed Reactor (PBR). Consequently, the design of a continuous biocatalytic production is a step towards a more competitive glycolipid synthesis in the aim for industrialization. |
format |
Text |
author |
Rebecca Hollenbach Delphine Muller André Delavault Christoph Syldatk |
author_facet |
Rebecca Hollenbach Delphine Muller André Delavault Christoph Syldatk |
author_sort |
Rebecca Hollenbach |
title |
Continuous Flow Glycolipid Synthesis Using a Packed Bed Reactor |
title_short |
Continuous Flow Glycolipid Synthesis Using a Packed Bed Reactor |
title_full |
Continuous Flow Glycolipid Synthesis Using a Packed Bed Reactor |
title_fullStr |
Continuous Flow Glycolipid Synthesis Using a Packed Bed Reactor |
title_full_unstemmed |
Continuous Flow Glycolipid Synthesis Using a Packed Bed Reactor |
title_sort |
continuous flow glycolipid synthesis using a packed bed reactor |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/catal12050551 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Catalysts; Volume 12; Issue 5; Pages: 551 |
op_relation |
Biocatalysis https://dx.doi.org/10.3390/catal12050551 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/catal12050551 |
container_title |
Catalysts |
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12 |
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5 |
container_start_page |
551 |
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1774712208736911360 |