Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp.
Phenol is an important pollutant widely discharged as a component of hydrocarbon fuels, but its degradation in cold regions is challenging due to the harsh environmental conditions. To date, there is little information available concerning the capability for phenol biodegradation by indigenous Antar...
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ftmdpi:oai:mdpi.com:/2073-4344/12/11/1422/ 2023-08-20T04:02:30+02:00 Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp. Gillian Li Yin Lee Nur Nadhirah Zakaria Hiroyuki Futamata Kenshi Suzuki Azham Zulkharnain Noor Azmi Shaharuddin Peter Convey Khadijah Nabilah Mohd Zahri Siti Aqlima Ahmad 2022-11-12 application/pdf https://doi.org/10.3390/catal12111422 EN eng Multidisciplinary Digital Publishing Institute Biocatalysis https://dx.doi.org/10.3390/catal12111422 https://creativecommons.org/licenses/by/4.0/ Catalysts; Volume 12; Issue 11; Pages: 1422 phenol metabolites whole genome sequencing xenobiotics Text 2022 ftmdpi https://doi.org/10.3390/catal12111422 2023-08-01T07:18:49Z Phenol is an important pollutant widely discharged as a component of hydrocarbon fuels, but its degradation in cold regions is challenging due to the harsh environmental conditions. To date, there is little information available concerning the capability for phenol biodegradation by indigenous Antarctic bacteria. In this study, enzyme activities and genes encoding phenol degradative enzymes identified using whole genome sequencing (WGS) were investigated to determine the pathway(s) of phenol degradation of Arthrobacter sp. strains AQ5-05 and AQ5-06, originally isolated from Antarctica. Complete phenol degradative genes involved only in the ortho-cleavage were detected in both strains. This was validated using assays of the enzymes catechol 1,2-dioxygenase and catechol 2,3-dioxygenase, which indicated the activity of only catechol 1,2-dioxygenase in both strains, in agreement with the results from the WGS. Both strains were psychrotolerant with the optimum temperature for phenol degradation, being between 10 and 15 °C. This study suggests the potential use of cold-adapted bacteria in the bioremediation of phenol pollution in cold environments. Text Antarc* Antarctic Antarctica MDPI Open Access Publishing Antarctic Catalysts 12 11 1422 |
institution |
Open Polar |
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MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
phenol metabolites whole genome sequencing xenobiotics |
spellingShingle |
phenol metabolites whole genome sequencing xenobiotics Gillian Li Yin Lee Nur Nadhirah Zakaria Hiroyuki Futamata Kenshi Suzuki Azham Zulkharnain Noor Azmi Shaharuddin Peter Convey Khadijah Nabilah Mohd Zahri Siti Aqlima Ahmad Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp. |
topic_facet |
phenol metabolites whole genome sequencing xenobiotics |
description |
Phenol is an important pollutant widely discharged as a component of hydrocarbon fuels, but its degradation in cold regions is challenging due to the harsh environmental conditions. To date, there is little information available concerning the capability for phenol biodegradation by indigenous Antarctic bacteria. In this study, enzyme activities and genes encoding phenol degradative enzymes identified using whole genome sequencing (WGS) were investigated to determine the pathway(s) of phenol degradation of Arthrobacter sp. strains AQ5-05 and AQ5-06, originally isolated from Antarctica. Complete phenol degradative genes involved only in the ortho-cleavage were detected in both strains. This was validated using assays of the enzymes catechol 1,2-dioxygenase and catechol 2,3-dioxygenase, which indicated the activity of only catechol 1,2-dioxygenase in both strains, in agreement with the results from the WGS. Both strains were psychrotolerant with the optimum temperature for phenol degradation, being between 10 and 15 °C. This study suggests the potential use of cold-adapted bacteria in the bioremediation of phenol pollution in cold environments. |
format |
Text |
author |
Gillian Li Yin Lee Nur Nadhirah Zakaria Hiroyuki Futamata Kenshi Suzuki Azham Zulkharnain Noor Azmi Shaharuddin Peter Convey Khadijah Nabilah Mohd Zahri Siti Aqlima Ahmad |
author_facet |
Gillian Li Yin Lee Nur Nadhirah Zakaria Hiroyuki Futamata Kenshi Suzuki Azham Zulkharnain Noor Azmi Shaharuddin Peter Convey Khadijah Nabilah Mohd Zahri Siti Aqlima Ahmad |
author_sort |
Gillian Li Yin Lee |
title |
Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp. |
title_short |
Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp. |
title_full |
Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp. |
title_fullStr |
Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp. |
title_full_unstemmed |
Metabolic Pathway of Phenol Degradation of a Cold-Adapted Antarctic Bacteria, Arthrobacter sp. |
title_sort |
metabolic pathway of phenol degradation of a cold-adapted antarctic bacteria, arthrobacter sp. |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/catal12111422 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Catalysts; Volume 12; Issue 11; Pages: 1422 |
op_relation |
Biocatalysis https://dx.doi.org/10.3390/catal12111422 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/catal12111422 |
container_title |
Catalysts |
container_volume |
12 |
container_issue |
11 |
container_start_page |
1422 |
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1774712976553541632 |