Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM
Residual oil from babassu (Orbignya sp.), a low-cost raw material, was used in the enzymatic esterification for biodiesel production, using lipase B from Candida antarctica (Novozym® 435) and ethanol. For the first time in the literature, residual babassu oil and Novozym® 435 are being investigated...
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ftmdpi:oai:mdpi.com:/2073-4344/10/4/414/ 2023-08-20T04:02:31+02:00 Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM Katerine S. Moreira Lourembergue S. Moura Júnior Rodolpho R. C. Monteiro André L. B. de Oliveira Camila P. Valle Tiago M. Freire Pierre B. A. Fechine Maria C. M. de Souza Gloria Fernandez-Lorente José M. Guisan José C. S. dos Santos 2020-04-09 application/pdf https://doi.org/10.3390/catal10040414 EN eng Multidisciplinary Digital Publishing Institute Biocatalysis https://dx.doi.org/10.3390/catal10040414 https://creativecommons.org/licenses/by/4.0/ Catalysts; Volume 10; Issue 4; Pages: 414 biodiesel Novozym ® 435 residual oil from babassu esterification response surface methodology Text 2020 ftmdpi https://doi.org/10.3390/catal10040414 2023-07-31T23:20:56Z Residual oil from babassu (Orbignya sp.), a low-cost raw material, was used in the enzymatic esterification for biodiesel production, using lipase B from Candida antarctica (Novozym® 435) and ethanol. For the first time in the literature, residual babassu oil and Novozym® 435 are being investigated to obtain biodiesel. In this communication, response surface methodology (RSM) and a central composite design (CCD) were used to optimize the esterification and study the effects of four factors (molar ratio (1:1–1:16, free fatty acids (FFAs) /alcohol), temperature (30–50 °C), biocatalyst content (0.05–0.15 g) and reaction time (2–6 h)) in the conversion into fatty acid ethyl esters. Under optimized conditions (1:18 molar ratio (FFAs/alcohol), 0.14 g of Novozym® 435, 48 °C and 4 h), the conversion into ethyl esters was 96.8%. It was found that after 10 consecutive cycles of esterification under optimal conditions, Novozym® 435 showed a maximum loss of activity of 5.8%, suggesting a very small change in the support/enzyme ratio proved by Fourier Transform Infrared (FTIR) spectroscopy and insignificant changes in the surface of Novozym® 435 proved by scanning electron microscopy (SEM) after the 10 consecutive cycles of esterification. Text Antarc* Antarctica MDPI Open Access Publishing Catalysts 10 4 414 |
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English |
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biodiesel Novozym ® 435 residual oil from babassu esterification response surface methodology |
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biodiesel Novozym ® 435 residual oil from babassu esterification response surface methodology Katerine S. Moreira Lourembergue S. Moura Júnior Rodolpho R. C. Monteiro André L. B. de Oliveira Camila P. Valle Tiago M. Freire Pierre B. A. Fechine Maria C. M. de Souza Gloria Fernandez-Lorente José M. Guisan José C. S. dos Santos Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM |
topic_facet |
biodiesel Novozym ® 435 residual oil from babassu esterification response surface methodology |
description |
Residual oil from babassu (Orbignya sp.), a low-cost raw material, was used in the enzymatic esterification for biodiesel production, using lipase B from Candida antarctica (Novozym® 435) and ethanol. For the first time in the literature, residual babassu oil and Novozym® 435 are being investigated to obtain biodiesel. In this communication, response surface methodology (RSM) and a central composite design (CCD) were used to optimize the esterification and study the effects of four factors (molar ratio (1:1–1:16, free fatty acids (FFAs) /alcohol), temperature (30–50 °C), biocatalyst content (0.05–0.15 g) and reaction time (2–6 h)) in the conversion into fatty acid ethyl esters. Under optimized conditions (1:18 molar ratio (FFAs/alcohol), 0.14 g of Novozym® 435, 48 °C and 4 h), the conversion into ethyl esters was 96.8%. It was found that after 10 consecutive cycles of esterification under optimal conditions, Novozym® 435 showed a maximum loss of activity of 5.8%, suggesting a very small change in the support/enzyme ratio proved by Fourier Transform Infrared (FTIR) spectroscopy and insignificant changes in the surface of Novozym® 435 proved by scanning electron microscopy (SEM) after the 10 consecutive cycles of esterification. |
format |
Text |
author |
Katerine S. Moreira Lourembergue S. Moura Júnior Rodolpho R. C. Monteiro André L. B. de Oliveira Camila P. Valle Tiago M. Freire Pierre B. A. Fechine Maria C. M. de Souza Gloria Fernandez-Lorente José M. Guisan José C. S. dos Santos |
author_facet |
Katerine S. Moreira Lourembergue S. Moura Júnior Rodolpho R. C. Monteiro André L. B. de Oliveira Camila P. Valle Tiago M. Freire Pierre B. A. Fechine Maria C. M. de Souza Gloria Fernandez-Lorente José M. Guisan José C. S. dos Santos |
author_sort |
Katerine S. Moreira |
title |
Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM |
title_short |
Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM |
title_full |
Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM |
title_fullStr |
Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM |
title_full_unstemmed |
Optimization of the Production of Enzymatic Biodiesel from Residual Babassu Oil (Orbignya sp.) via RSM |
title_sort |
optimization of the production of enzymatic biodiesel from residual babassu oil (orbignya sp.) via rsm |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/catal10040414 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Catalysts; Volume 10; Issue 4; Pages: 414 |
op_relation |
Biocatalysis https://dx.doi.org/10.3390/catal10040414 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/catal10040414 |
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Catalysts |
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10 |
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414 |
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