Toxic Cyanobacteria in Svalbard: Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method
Cyanobacteria synthesize a large variety of secondary metabolites including toxins. Microcystins (MCs) with hepato- and neurotoxic potential are well studied in bloom-forming planktonic species of temperate and tropical regions. Cyanobacterial biofilms thriving in the polar regions have recently eme...
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ftmdpi:oai:mdpi.com:/2072-6651/10/4/147/ 2023-08-20T04:02:01+02:00 Toxic Cyanobacteria in Svalbard: Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method Julia Kleinteich Jonathan Puddick Susanna Wood Falk Hildebrand H. Laughinghouse IV David Pearce Daniel Dietrich Annick Wilmotte agris 2018-04-03 application/pdf https://doi.org/10.3390/toxins10040147 EN eng Multidisciplinary Digital Publishing Institute Marine and Freshwater Toxins https://dx.doi.org/10.3390/toxins10040147 https://creativecommons.org/licenses/by/4.0/ Toxins; Volume 10; Issue 4; Pages: 147 arctic benthic mats cyanotoxins ELISA 16S rRNA gene Text 2018 ftmdpi https://doi.org/10.3390/toxins10040147 2023-07-31T21:27:31Z Cyanobacteria synthesize a large variety of secondary metabolites including toxins. Microcystins (MCs) with hepato- and neurotoxic potential are well studied in bloom-forming planktonic species of temperate and tropical regions. Cyanobacterial biofilms thriving in the polar regions have recently emerged as a rich source for cyanobacterial secondary metabolites including previously undescribed congeners of microcystin. However, detection and detailed identification of these compounds is difficult due to unusual sample matrices and structural congeners produced. We here report a time-efficient liquid chromatography-mass spectrometry (LC-MS) precursor ion screening method that facilitates microcystin detection and identification. We applied this method to detect six different MC congeners in 8 out of 26 microbial mat samples of the Svalbard Archipelago in the Arctic. The congeners, of which [Asp3, ADMAdda5, Dhb7] MC-LR was most abundant, were similar to those reported in other polar habitats. Microcystins were also determined using an Adda-specific enzyme-linked immunosorbent assay (Adda-ELISA). Nostoc sp. was identified as a putative toxin producer using molecular methods that targeted 16S rRNA genes and genes involved in microcystin production. The mcy genes detected showed highest similarities to other Arctic or Antarctic sequences. The LC-MS precursor ion screening method could be useful for microcystin detection in unusual matrices such as benthic biofilms or lichen. Text Antarc* Antarctic Arctic Svalbard MDPI Open Access Publishing Antarctic Arctic Svalbard Svalbard Archipelago Toxins 10 4 147 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
arctic benthic mats cyanotoxins ELISA 16S rRNA gene |
spellingShingle |
arctic benthic mats cyanotoxins ELISA 16S rRNA gene Julia Kleinteich Jonathan Puddick Susanna Wood Falk Hildebrand H. Laughinghouse IV David Pearce Daniel Dietrich Annick Wilmotte Toxic Cyanobacteria in Svalbard: Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method |
topic_facet |
arctic benthic mats cyanotoxins ELISA 16S rRNA gene |
description |
Cyanobacteria synthesize a large variety of secondary metabolites including toxins. Microcystins (MCs) with hepato- and neurotoxic potential are well studied in bloom-forming planktonic species of temperate and tropical regions. Cyanobacterial biofilms thriving in the polar regions have recently emerged as a rich source for cyanobacterial secondary metabolites including previously undescribed congeners of microcystin. However, detection and detailed identification of these compounds is difficult due to unusual sample matrices and structural congeners produced. We here report a time-efficient liquid chromatography-mass spectrometry (LC-MS) precursor ion screening method that facilitates microcystin detection and identification. We applied this method to detect six different MC congeners in 8 out of 26 microbial mat samples of the Svalbard Archipelago in the Arctic. The congeners, of which [Asp3, ADMAdda5, Dhb7] MC-LR was most abundant, were similar to those reported in other polar habitats. Microcystins were also determined using an Adda-specific enzyme-linked immunosorbent assay (Adda-ELISA). Nostoc sp. was identified as a putative toxin producer using molecular methods that targeted 16S rRNA genes and genes involved in microcystin production. The mcy genes detected showed highest similarities to other Arctic or Antarctic sequences. The LC-MS precursor ion screening method could be useful for microcystin detection in unusual matrices such as benthic biofilms or lichen. |
format |
Text |
author |
Julia Kleinteich Jonathan Puddick Susanna Wood Falk Hildebrand H. Laughinghouse IV David Pearce Daniel Dietrich Annick Wilmotte |
author_facet |
Julia Kleinteich Jonathan Puddick Susanna Wood Falk Hildebrand H. Laughinghouse IV David Pearce Daniel Dietrich Annick Wilmotte |
author_sort |
Julia Kleinteich |
title |
Toxic Cyanobacteria in Svalbard: Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method |
title_short |
Toxic Cyanobacteria in Svalbard: Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method |
title_full |
Toxic Cyanobacteria in Svalbard: Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method |
title_fullStr |
Toxic Cyanobacteria in Svalbard: Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method |
title_full_unstemmed |
Toxic Cyanobacteria in Svalbard: Chemical Diversity of Microcystins Detected Using a Liquid Chromatography Mass Spectrometry Precursor Ion Screening Method |
title_sort |
toxic cyanobacteria in svalbard: chemical diversity of microcystins detected using a liquid chromatography mass spectrometry precursor ion screening method |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2018 |
url |
https://doi.org/10.3390/toxins10040147 |
op_coverage |
agris |
geographic |
Antarctic Arctic Svalbard Svalbard Archipelago |
geographic_facet |
Antarctic Arctic Svalbard Svalbard Archipelago |
genre |
Antarc* Antarctic Arctic Svalbard |
genre_facet |
Antarc* Antarctic Arctic Svalbard |
op_source |
Toxins; Volume 10; Issue 4; Pages: 147 |
op_relation |
Marine and Freshwater Toxins https://dx.doi.org/10.3390/toxins10040147 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/toxins10040147 |
container_title |
Toxins |
container_volume |
10 |
container_issue |
4 |
container_start_page |
147 |
_version_ |
1774712409586401280 |