Carbazole Degradation and Genetic Analyses of Sphingobium sp. Strain BS19 Isolated from Antarctic Soil
The Antarctic region is facing a higher risk of hydrocarbon pollution due to increased human activities. Compounds such as polycyclic aromatic hydrocarbons (PAHs) and heterocyclic compounds available in fuel are highly stable and can reside in the environment for prolonged periods if left untreated....
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ftmdpi:oai:mdpi.com:/2071-1050/15/9/7197/ 2023-08-20T04:01:32+02:00 Carbazole Degradation and Genetic Analyses of Sphingobium sp. Strain BS19 Isolated from Antarctic Soil Kenta Sato Seiryu Take Siti Aqlima Ahmad Claudio Gomez-Fuentes Azham Zulkharnain agris 2023-04-26 application/pdf https://doi.org/10.3390/su15097197 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/su15097197 https://creativecommons.org/licenses/by/4.0/ Sustainability; Volume 15; Issue 9; Pages: 7197 carbazole bioremediation Antarctic bacterium Text 2023 ftmdpi https://doi.org/10.3390/su15097197 2023-08-01T09:51:04Z The Antarctic region is facing a higher risk of hydrocarbon pollution due to increased human activities. Compounds such as polycyclic aromatic hydrocarbons (PAHs) and heterocyclic compounds available in fuel are highly stable and can reside in the environment for prolonged periods if left untreated. The isolation of native strains is needed to develop bioremediation applications suitable for Antarctica. Strain BS19 was isolated as heterocyclic compound carbazole-degrading bacterium from Antarctic soil through culture enrichment. The 16S rRNA gene sequences identified strain BS19 as a member of the Sphingonium genus. Strain BS19 could remove 75% of carbazole after 15 days of culture at 15 °C. Whole genome sequencing resulted in incomplete genomes of 4.77 Mb in 96 contigs with the lowest GC content among Sphingobium sp. strains. The analyses revealed car gene cluster and ant genes and cat gene cluster required for the complete metabolism of carbazole as a source of carbon and energy. The comparison of the car gene cluster showed a similarity to the car gene cluster of Novosphingobium KA1. The expression of the car gene cluster was confirmed with an RT-PCR analysis indicating the involvement of the predicted genes in carbazole degradation. The findings from this study could provide more insight into developing bioremediation applications and approaches for Antarctica and other cold environments. Text Antarc* Antarctic Antarctica MDPI Open Access Publishing Antarctic The Antarctic Sustainability 15 9 7197 |
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carbazole bioremediation Antarctic bacterium |
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carbazole bioremediation Antarctic bacterium Kenta Sato Seiryu Take Siti Aqlima Ahmad Claudio Gomez-Fuentes Azham Zulkharnain Carbazole Degradation and Genetic Analyses of Sphingobium sp. Strain BS19 Isolated from Antarctic Soil |
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carbazole bioremediation Antarctic bacterium |
description |
The Antarctic region is facing a higher risk of hydrocarbon pollution due to increased human activities. Compounds such as polycyclic aromatic hydrocarbons (PAHs) and heterocyclic compounds available in fuel are highly stable and can reside in the environment for prolonged periods if left untreated. The isolation of native strains is needed to develop bioremediation applications suitable for Antarctica. Strain BS19 was isolated as heterocyclic compound carbazole-degrading bacterium from Antarctic soil through culture enrichment. The 16S rRNA gene sequences identified strain BS19 as a member of the Sphingonium genus. Strain BS19 could remove 75% of carbazole after 15 days of culture at 15 °C. Whole genome sequencing resulted in incomplete genomes of 4.77 Mb in 96 contigs with the lowest GC content among Sphingobium sp. strains. The analyses revealed car gene cluster and ant genes and cat gene cluster required for the complete metabolism of carbazole as a source of carbon and energy. The comparison of the car gene cluster showed a similarity to the car gene cluster of Novosphingobium KA1. The expression of the car gene cluster was confirmed with an RT-PCR analysis indicating the involvement of the predicted genes in carbazole degradation. The findings from this study could provide more insight into developing bioremediation applications and approaches for Antarctica and other cold environments. |
format |
Text |
author |
Kenta Sato Seiryu Take Siti Aqlima Ahmad Claudio Gomez-Fuentes Azham Zulkharnain |
author_facet |
Kenta Sato Seiryu Take Siti Aqlima Ahmad Claudio Gomez-Fuentes Azham Zulkharnain |
author_sort |
Kenta Sato |
title |
Carbazole Degradation and Genetic Analyses of Sphingobium sp. Strain BS19 Isolated from Antarctic Soil |
title_short |
Carbazole Degradation and Genetic Analyses of Sphingobium sp. Strain BS19 Isolated from Antarctic Soil |
title_full |
Carbazole Degradation and Genetic Analyses of Sphingobium sp. Strain BS19 Isolated from Antarctic Soil |
title_fullStr |
Carbazole Degradation and Genetic Analyses of Sphingobium sp. Strain BS19 Isolated from Antarctic Soil |
title_full_unstemmed |
Carbazole Degradation and Genetic Analyses of Sphingobium sp. Strain BS19 Isolated from Antarctic Soil |
title_sort |
carbazole degradation and genetic analyses of sphingobium sp. strain bs19 isolated from antarctic soil |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/su15097197 |
op_coverage |
agris |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
Sustainability; Volume 15; Issue 9; Pages: 7197 |
op_relation |
https://dx.doi.org/10.3390/su15097197 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/su15097197 |
container_title |
Sustainability |
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15 |
container_issue |
9 |
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7197 |
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1774724792599969792 |