A Comprehensive Metagenomic Analysis Framework Revealing Microbiome Profile and Potential for Hydrocarbon Degradation and Carbohydrate Metabolism in a Himalayan Artificial Lake
The high-altitude lakes are ecological habitats accommodating a vast diversity of microbial populations. These microbes are efficient sources for a variety of enzymes. The objective of this study is to perform in-depth metagenomic profiling of an artificial lake ecosystem located in the Sikkim Himal...
Published in: | Sustainability |
---|---|
Main Authors: | , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2022
|
Subjects: | |
Online Access: | https://doi.org/10.3390/su141811455 |
id |
ftmdpi:oai:mdpi.com:/2071-1050/14/18/11455/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/2071-1050/14/18/11455/ 2023-08-20T04:00:34+02:00 A Comprehensive Metagenomic Analysis Framework Revealing Microbiome Profile and Potential for Hydrocarbon Degradation and Carbohydrate Metabolism in a Himalayan Artificial Lake Girija Kaushal Monika Thakur Amit Kumar Rai Sudhir P. Singh agris 2022-09-13 application/pdf https://doi.org/10.3390/su141811455 EN eng Multidisciplinary Digital Publishing Institute Sustainable Materials https://dx.doi.org/10.3390/su141811455 https://creativecommons.org/licenses/by/4.0/ Sustainability; Volume 14; Issue 18; Pages: 11455 high-altitude artificial lake Himalayan metagenome β-galactosidase hydrocarbon degradation Text 2022 ftmdpi https://doi.org/10.3390/su141811455 2023-08-01T06:27:19Z The high-altitude lakes are ecological habitats accommodating a vast diversity of microbial populations. These microbes are efficient sources for a variety of enzymes. The objective of this study is to perform in-depth metagenomic profiling of an artificial lake ecosystem located in the Sikkim Himalayan region, deciphering the hydrocarbon degradation potential of this site and mining biocatalysts of industrial importance. In the present study, metagenomic analysis of an artificial Himalayan lake, located in North Sikkim, India, was performed. A comprehensive taxonomic and functional profiling revealed gene mapped to pathways for degradation of hydrocarbons such as toluene, benzoate, ethylbenzene, etc. This site was rich in iron, and the metagenomic investigation revealed genomic signatures of the iron-reducing bacterium; Geothrix fermentans. The appraisal of the carbohydrate metabolic potential of this site divulged the predominance of β-galactosidase genes. The artificial lake metagenome was further compared to publicly available saline and freshwater lakes. At the taxonomic, as well as functional levels, it was found to be closer to freshwater lake metagenome, e.g., Medonta Lake, US, and freshwater Vanda Lake, Antarctica. The microbial community profiling and functional contribution of the artificial Himalayan lake would be beneficial for mining genes encoding various industrially relevant enzymes. Text Antarc* Antarctica MDPI Open Access Publishing Vanda ENVELOPE(161.550,161.550,-77.533,-77.533) Vanda Lake ENVELOPE(161.550,161.550,-77.533,-77.533) Sustainability 14 18 11455 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
high-altitude artificial lake Himalayan metagenome β-galactosidase hydrocarbon degradation |
spellingShingle |
high-altitude artificial lake Himalayan metagenome β-galactosidase hydrocarbon degradation Girija Kaushal Monika Thakur Amit Kumar Rai Sudhir P. Singh A Comprehensive Metagenomic Analysis Framework Revealing Microbiome Profile and Potential for Hydrocarbon Degradation and Carbohydrate Metabolism in a Himalayan Artificial Lake |
topic_facet |
high-altitude artificial lake Himalayan metagenome β-galactosidase hydrocarbon degradation |
description |
The high-altitude lakes are ecological habitats accommodating a vast diversity of microbial populations. These microbes are efficient sources for a variety of enzymes. The objective of this study is to perform in-depth metagenomic profiling of an artificial lake ecosystem located in the Sikkim Himalayan region, deciphering the hydrocarbon degradation potential of this site and mining biocatalysts of industrial importance. In the present study, metagenomic analysis of an artificial Himalayan lake, located in North Sikkim, India, was performed. A comprehensive taxonomic and functional profiling revealed gene mapped to pathways for degradation of hydrocarbons such as toluene, benzoate, ethylbenzene, etc. This site was rich in iron, and the metagenomic investigation revealed genomic signatures of the iron-reducing bacterium; Geothrix fermentans. The appraisal of the carbohydrate metabolic potential of this site divulged the predominance of β-galactosidase genes. The artificial lake metagenome was further compared to publicly available saline and freshwater lakes. At the taxonomic, as well as functional levels, it was found to be closer to freshwater lake metagenome, e.g., Medonta Lake, US, and freshwater Vanda Lake, Antarctica. The microbial community profiling and functional contribution of the artificial Himalayan lake would be beneficial for mining genes encoding various industrially relevant enzymes. |
format |
Text |
author |
Girija Kaushal Monika Thakur Amit Kumar Rai Sudhir P. Singh |
author_facet |
Girija Kaushal Monika Thakur Amit Kumar Rai Sudhir P. Singh |
author_sort |
Girija Kaushal |
title |
A Comprehensive Metagenomic Analysis Framework Revealing Microbiome Profile and Potential for Hydrocarbon Degradation and Carbohydrate Metabolism in a Himalayan Artificial Lake |
title_short |
A Comprehensive Metagenomic Analysis Framework Revealing Microbiome Profile and Potential for Hydrocarbon Degradation and Carbohydrate Metabolism in a Himalayan Artificial Lake |
title_full |
A Comprehensive Metagenomic Analysis Framework Revealing Microbiome Profile and Potential for Hydrocarbon Degradation and Carbohydrate Metabolism in a Himalayan Artificial Lake |
title_fullStr |
A Comprehensive Metagenomic Analysis Framework Revealing Microbiome Profile and Potential for Hydrocarbon Degradation and Carbohydrate Metabolism in a Himalayan Artificial Lake |
title_full_unstemmed |
A Comprehensive Metagenomic Analysis Framework Revealing Microbiome Profile and Potential for Hydrocarbon Degradation and Carbohydrate Metabolism in a Himalayan Artificial Lake |
title_sort |
comprehensive metagenomic analysis framework revealing microbiome profile and potential for hydrocarbon degradation and carbohydrate metabolism in a himalayan artificial lake |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/su141811455 |
op_coverage |
agris |
long_lat |
ENVELOPE(161.550,161.550,-77.533,-77.533) ENVELOPE(161.550,161.550,-77.533,-77.533) |
geographic |
Vanda Vanda Lake |
geographic_facet |
Vanda Vanda Lake |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Sustainability; Volume 14; Issue 18; Pages: 11455 |
op_relation |
Sustainable Materials https://dx.doi.org/10.3390/su141811455 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/su141811455 |
container_title |
Sustainability |
container_volume |
14 |
container_issue |
18 |
container_start_page |
11455 |
_version_ |
1774718947719905280 |