Thrombolytic Potential of Micromycetes from the Genus Tolypocladium, Obtained from White Sea Soils: Screening of Producers and Exoproteinases Properties
Thrombotherapy is an important approach in treatment of various diseases associated with pathologies of the cardiovascular and human hemostasis systems. Screening for producers of modern, specific, and safe thrombolytic substances is an important task for medicine and biotechnology. The aim of this...
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ftmdpi:oai:mdpi.com:/2036-7481/13/4/63/ 2023-08-20T04:10:19+02:00 Thrombolytic Potential of Micromycetes from the Genus Tolypocladium, Obtained from White Sea Soils: Screening of Producers and Exoproteinases Properties N. S. Fokichev L. Yu. Kokaeva E. A. Popova A. V. Kurakov A. A. Osmolovskiy 2022-11-07 application/pdf https://doi.org/10.3390/microbiolres13040063 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/microbiolres13040063 https://creativecommons.org/licenses/by/4.0/ Microbiology Research; Volume 13; Issue 4; Pages: 898-908 thrombotherapy micromycetes Tolypocladium extracellular proteases thrombolysis Text 2022 ftmdpi https://doi.org/10.3390/microbiolres13040063 2023-08-01T07:13:12Z Thrombotherapy is an important approach in treatment of various diseases associated with pathologies of the cardiovascular and human hemostasis systems. Screening for producers of modern, specific, and safe thrombolytic substances is an important task for medicine and biotechnology. The aim of this study was to characterize thrombolytic potential of seven strains of micromycete belonging to the genus Tolypocladium, which was obtained from White Sea soils. The Tolypocladium inflatum 62a strain was considered the most promising producer of thrombolytic agent activities suitable for possible use in thrombotherapy or diagnostics of hemostasis pathologies. It demonstrated a high radial growth rate and was characterized not only by a sufficiently high value of enzymatic index in media with fibrin and fibrinogen but also by the highest specificity for fibrillar proteins among all strains. The preparation obtained from it demonstrated pronounced thrombolytic effectiveness and substrate specificity. Text White Sea MDPI Open Access Publishing White Sea Microbiology Research 13 4 898 908 |
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English |
topic |
thrombotherapy micromycetes Tolypocladium extracellular proteases thrombolysis |
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thrombotherapy micromycetes Tolypocladium extracellular proteases thrombolysis N. S. Fokichev L. Yu. Kokaeva E. A. Popova A. V. Kurakov A. A. Osmolovskiy Thrombolytic Potential of Micromycetes from the Genus Tolypocladium, Obtained from White Sea Soils: Screening of Producers and Exoproteinases Properties |
topic_facet |
thrombotherapy micromycetes Tolypocladium extracellular proteases thrombolysis |
description |
Thrombotherapy is an important approach in treatment of various diseases associated with pathologies of the cardiovascular and human hemostasis systems. Screening for producers of modern, specific, and safe thrombolytic substances is an important task for medicine and biotechnology. The aim of this study was to characterize thrombolytic potential of seven strains of micromycete belonging to the genus Tolypocladium, which was obtained from White Sea soils. The Tolypocladium inflatum 62a strain was considered the most promising producer of thrombolytic agent activities suitable for possible use in thrombotherapy or diagnostics of hemostasis pathologies. It demonstrated a high radial growth rate and was characterized not only by a sufficiently high value of enzymatic index in media with fibrin and fibrinogen but also by the highest specificity for fibrillar proteins among all strains. The preparation obtained from it demonstrated pronounced thrombolytic effectiveness and substrate specificity. |
format |
Text |
author |
N. S. Fokichev L. Yu. Kokaeva E. A. Popova A. V. Kurakov A. A. Osmolovskiy |
author_facet |
N. S. Fokichev L. Yu. Kokaeva E. A. Popova A. V. Kurakov A. A. Osmolovskiy |
author_sort |
N. S. Fokichev |
title |
Thrombolytic Potential of Micromycetes from the Genus Tolypocladium, Obtained from White Sea Soils: Screening of Producers and Exoproteinases Properties |
title_short |
Thrombolytic Potential of Micromycetes from the Genus Tolypocladium, Obtained from White Sea Soils: Screening of Producers and Exoproteinases Properties |
title_full |
Thrombolytic Potential of Micromycetes from the Genus Tolypocladium, Obtained from White Sea Soils: Screening of Producers and Exoproteinases Properties |
title_fullStr |
Thrombolytic Potential of Micromycetes from the Genus Tolypocladium, Obtained from White Sea Soils: Screening of Producers and Exoproteinases Properties |
title_full_unstemmed |
Thrombolytic Potential of Micromycetes from the Genus Tolypocladium, Obtained from White Sea Soils: Screening of Producers and Exoproteinases Properties |
title_sort |
thrombolytic potential of micromycetes from the genus tolypocladium, obtained from white sea soils: screening of producers and exoproteinases properties |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/microbiolres13040063 |
geographic |
White Sea |
geographic_facet |
White Sea |
genre |
White Sea |
genre_facet |
White Sea |
op_source |
Microbiology Research; Volume 13; Issue 4; Pages: 898-908 |
op_relation |
https://dx.doi.org/10.3390/microbiolres13040063 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/microbiolres13040063 |
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Microbiology Research |
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13 |
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4 |
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898 |
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908 |
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1774724416023822336 |