A Polyprotein-Expressing Salmonid Alphavirus Replicon Induces Modest Protection in Atlantic Salmon (Salmo Salar) Against Infectious Pancreatic Necrosis
Vaccination is an important strategy for the control and prevention of infectious pancreatic necrosis (IPN) in farmed Atlantic salmon (Salmo salar) in the post-smolt stage in sea-water. In this study, a heterologous gene expression system, based on a replicon construct of salmonid alphavirus (SAV),...
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ftmdpi:oai:mdpi.com:/1999-4915/7/1/252/ 2023-08-20T04:05:14+02:00 A Polyprotein-Expressing Salmonid Alphavirus Replicon Induces Modest Protection in Atlantic Salmon (Salmo Salar) Against Infectious Pancreatic Necrosis Azila Abdullah Christel Olsen Kjartan Hodneland Espen Rimstad agris 2015-01-19 application/pdf https://doi.org/10.3390/v7010252 EN eng Multidisciplinary Digital Publishing Institute Viral Immunology, Vaccines, and Antivirals https://dx.doi.org/10.3390/v7010252 https://creativecommons.org/licenses/by/4.0/ Viruses; Volume 7; Issue 1; Pages: 252-267 infectious pancreatic necrosis Atlantic salmon vaccination alphavirus replicon Text 2015 ftmdpi https://doi.org/10.3390/v7010252 2023-07-31T20:41:24Z Vaccination is an important strategy for the control and prevention of infectious pancreatic necrosis (IPN) in farmed Atlantic salmon (Salmo salar) in the post-smolt stage in sea-water. In this study, a heterologous gene expression system, based on a replicon construct of salmonid alphavirus (SAV), was used for in vitro and in vivo expression of IPN virus proteins. The large open reading frame of segment A, encoding the polyprotein NH2-pVP2-VP4-VP3-COOH, as well as pVP2, were cloned and expressed by the SAV replicon in Chinook salmon embryo cells (CHSE-214) and epithelioma papulosum cyprini (EPC) cells. The replicon constructs pSAV/polyprotein (pSAV/PP) and pSAV/pVP2 were used to immunize Atlantic salmon (Salmo salar) by a single intramuscular injection and tested in a subsequent IPN virus (IPNV) challenge trial. A low to moderate protection against IPN was observed in fish immunized with the replicon vaccine that encoded the pSAV/PP, while the pSAV/pVP2 construct was not found to induce protection. Text Atlantic salmon Salmo salar MDPI Open Access Publishing Sav’ ENVELOPE(156.400,156.400,68.817,68.817) Viruses 7 1 252 267 |
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MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
infectious pancreatic necrosis Atlantic salmon vaccination alphavirus replicon |
spellingShingle |
infectious pancreatic necrosis Atlantic salmon vaccination alphavirus replicon Azila Abdullah Christel Olsen Kjartan Hodneland Espen Rimstad A Polyprotein-Expressing Salmonid Alphavirus Replicon Induces Modest Protection in Atlantic Salmon (Salmo Salar) Against Infectious Pancreatic Necrosis |
topic_facet |
infectious pancreatic necrosis Atlantic salmon vaccination alphavirus replicon |
description |
Vaccination is an important strategy for the control and prevention of infectious pancreatic necrosis (IPN) in farmed Atlantic salmon (Salmo salar) in the post-smolt stage in sea-water. In this study, a heterologous gene expression system, based on a replicon construct of salmonid alphavirus (SAV), was used for in vitro and in vivo expression of IPN virus proteins. The large open reading frame of segment A, encoding the polyprotein NH2-pVP2-VP4-VP3-COOH, as well as pVP2, were cloned and expressed by the SAV replicon in Chinook salmon embryo cells (CHSE-214) and epithelioma papulosum cyprini (EPC) cells. The replicon constructs pSAV/polyprotein (pSAV/PP) and pSAV/pVP2 were used to immunize Atlantic salmon (Salmo salar) by a single intramuscular injection and tested in a subsequent IPN virus (IPNV) challenge trial. A low to moderate protection against IPN was observed in fish immunized with the replicon vaccine that encoded the pSAV/PP, while the pSAV/pVP2 construct was not found to induce protection. |
format |
Text |
author |
Azila Abdullah Christel Olsen Kjartan Hodneland Espen Rimstad |
author_facet |
Azila Abdullah Christel Olsen Kjartan Hodneland Espen Rimstad |
author_sort |
Azila Abdullah |
title |
A Polyprotein-Expressing Salmonid Alphavirus Replicon Induces Modest Protection in Atlantic Salmon (Salmo Salar) Against Infectious Pancreatic Necrosis |
title_short |
A Polyprotein-Expressing Salmonid Alphavirus Replicon Induces Modest Protection in Atlantic Salmon (Salmo Salar) Against Infectious Pancreatic Necrosis |
title_full |
A Polyprotein-Expressing Salmonid Alphavirus Replicon Induces Modest Protection in Atlantic Salmon (Salmo Salar) Against Infectious Pancreatic Necrosis |
title_fullStr |
A Polyprotein-Expressing Salmonid Alphavirus Replicon Induces Modest Protection in Atlantic Salmon (Salmo Salar) Against Infectious Pancreatic Necrosis |
title_full_unstemmed |
A Polyprotein-Expressing Salmonid Alphavirus Replicon Induces Modest Protection in Atlantic Salmon (Salmo Salar) Against Infectious Pancreatic Necrosis |
title_sort |
polyprotein-expressing salmonid alphavirus replicon induces modest protection in atlantic salmon (salmo salar) against infectious pancreatic necrosis |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2015 |
url |
https://doi.org/10.3390/v7010252 |
op_coverage |
agris |
long_lat |
ENVELOPE(156.400,156.400,68.817,68.817) |
geographic |
Sav’ |
geographic_facet |
Sav’ |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Viruses; Volume 7; Issue 1; Pages: 252-267 |
op_relation |
Viral Immunology, Vaccines, and Antivirals https://dx.doi.org/10.3390/v7010252 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/v7010252 |
container_title |
Viruses |
container_volume |
7 |
container_issue |
1 |
container_start_page |
252 |
op_container_end_page |
267 |
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1774715715059712000 |