Detection of Coronaviruses in Bats of Various Species in Italy
Bats are natural reservoirs for many mammalian coronaviruses, which have received renewed interest after the discovery of the severe acute respiratory syndrome (SARS) and the Middle East respiratory syndrome (MERS) CoV in humans. This study describes the identification and molecular characterization...
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ftmdpi:oai:mdpi.com:/1999-4915/5/11/2679/ 2023-08-20T04:08:52+02:00 Detection of Coronaviruses in Bats of Various Species in Italy Davide Lelli Alice Papetti Cristiano Sabelli Enrica Rosti Ana Moreno Maria Boniotti agris 2013-10-31 application/pdf https://doi.org/10.3390/v5112679 EN eng Multidisciplinary Digital Publishing Institute Animal Viruses https://dx.doi.org/10.3390/v5112679 https://creativecommons.org/licenses/by/3.0/ Viruses; Volume 5; Issue 11; Pages: 2679-2689 coronavirus bats Italy molecular characterization phylogenetic analysis Text 2013 ftmdpi https://doi.org/10.3390/v5112679 2023-07-31T20:34:33Z Bats are natural reservoirs for many mammalian coronaviruses, which have received renewed interest after the discovery of the severe acute respiratory syndrome (SARS) and the Middle East respiratory syndrome (MERS) CoV in humans. This study describes the identification and molecular characterization of alphacoronaviruses and betacoronaviruses in bats in Italy, from 2010 to 2012. Sixty-nine faecal samples and 126 carcasses were tested using pan-coronavirus RT-PCR. Coronavirus RNAs were detected in seven faecal samples and nine carcasses. A phylogenetic analysis of RNA-dependent RNA polymerase sequence fragments aided in identifying two alphacoronaviruses from Kuhl’s pipistrelle (Pipistrellus kuhlii), three clade 2b betacoronaviruses from lesser horseshoe bats (Rhinolophus hipposideros), and 10 clade 2c betacoronaviruses from Kuhl’s pipistrelle, common noctule (Nyctalus noctula), and Savi’s pipistrelle (Hypsugo savii). This study fills a substantive gap in the knowledge on bat-CoV ecology in Italy, and extends the current knowledge on clade 2c betacoronaviruses with new sequences obtained from bats that have not been previously described as hosts of these viruses. Text Nyctalus noctula MDPI Open Access Publishing Viruses 5 11 2679 2689 |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
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coronavirus bats Italy molecular characterization phylogenetic analysis |
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coronavirus bats Italy molecular characterization phylogenetic analysis Davide Lelli Alice Papetti Cristiano Sabelli Enrica Rosti Ana Moreno Maria Boniotti Detection of Coronaviruses in Bats of Various Species in Italy |
topic_facet |
coronavirus bats Italy molecular characterization phylogenetic analysis |
description |
Bats are natural reservoirs for many mammalian coronaviruses, which have received renewed interest after the discovery of the severe acute respiratory syndrome (SARS) and the Middle East respiratory syndrome (MERS) CoV in humans. This study describes the identification and molecular characterization of alphacoronaviruses and betacoronaviruses in bats in Italy, from 2010 to 2012. Sixty-nine faecal samples and 126 carcasses were tested using pan-coronavirus RT-PCR. Coronavirus RNAs were detected in seven faecal samples and nine carcasses. A phylogenetic analysis of RNA-dependent RNA polymerase sequence fragments aided in identifying two alphacoronaviruses from Kuhl’s pipistrelle (Pipistrellus kuhlii), three clade 2b betacoronaviruses from lesser horseshoe bats (Rhinolophus hipposideros), and 10 clade 2c betacoronaviruses from Kuhl’s pipistrelle, common noctule (Nyctalus noctula), and Savi’s pipistrelle (Hypsugo savii). This study fills a substantive gap in the knowledge on bat-CoV ecology in Italy, and extends the current knowledge on clade 2c betacoronaviruses with new sequences obtained from bats that have not been previously described as hosts of these viruses. |
format |
Text |
author |
Davide Lelli Alice Papetti Cristiano Sabelli Enrica Rosti Ana Moreno Maria Boniotti |
author_facet |
Davide Lelli Alice Papetti Cristiano Sabelli Enrica Rosti Ana Moreno Maria Boniotti |
author_sort |
Davide Lelli |
title |
Detection of Coronaviruses in Bats of Various Species in Italy |
title_short |
Detection of Coronaviruses in Bats of Various Species in Italy |
title_full |
Detection of Coronaviruses in Bats of Various Species in Italy |
title_fullStr |
Detection of Coronaviruses in Bats of Various Species in Italy |
title_full_unstemmed |
Detection of Coronaviruses in Bats of Various Species in Italy |
title_sort |
detection of coronaviruses in bats of various species in italy |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2013 |
url |
https://doi.org/10.3390/v5112679 |
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agris |
genre |
Nyctalus noctula |
genre_facet |
Nyctalus noctula |
op_source |
Viruses; Volume 5; Issue 11; Pages: 2679-2689 |
op_relation |
Animal Viruses https://dx.doi.org/10.3390/v5112679 |
op_rights |
https://creativecommons.org/licenses/by/3.0/ |
op_doi |
https://doi.org/10.3390/v5112679 |
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Viruses |
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5 |
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11 |
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2679 |
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2689 |
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1774721414495993856 |