Identification of Potential Proteinaceous Ligands of GI.1 Norovirus in Pacific Oyster Tissues
Human norovirus (HuNoV) is the leading foodborne pathogen causing nonbacterial gastroenteritis worldwide. The oyster is an important vehicle for HuNoV transmission, especially the GI.1 HuNoV. In our previous study, oyster heat shock protein 70 (oHSP 70) was identified as the first proteinaceous liga...
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Multidisciplinary Digital Publishing Institute
2023
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Online Access: | https://doi.org/10.3390/v15030631 |
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ftmdpi:oai:mdpi.com:/1999-4915/15/3/631/ 2023-08-20T04:06:04+02:00 Identification of Potential Proteinaceous Ligands of GI.1 Norovirus in Pacific Oyster Tissues Chenang Lyu Jingwen Li Zhentao Shi Ran An Yanfei Wang Guangda Luo Dapeng Wang agris 2023-02-25 application/pdf https://doi.org/10.3390/v15030631 EN eng Multidisciplinary Digital Publishing Institute Human Virology and Viral Diseases https://dx.doi.org/10.3390/v15030631 https://creativecommons.org/licenses/by/4.0/ Viruses; Volume 15; Issue 3; Pages: 631 human norovirus Crassostrea gigas specific binding ligands tumor necrosis factor intraflagellar transport protein Text 2023 ftmdpi https://doi.org/10.3390/v15030631 2023-08-01T08:59:38Z Human norovirus (HuNoV) is the leading foodborne pathogen causing nonbacterial gastroenteritis worldwide. The oyster is an important vehicle for HuNoV transmission, especially the GI.1 HuNoV. In our previous study, oyster heat shock protein 70 (oHSP 70) was identified as the first proteinaceous ligand of GII.4 HuNoV in Pacific oysters besides the commonly accepted carbohydrate ligands, a histo-blood group antigens (HBGAs)-like substance. However the mismatch of the distribution pattern between discovered ligands and GI.1 HuNoV suggests that other ligands may exist. In our study, proteinaceous ligands for the specific binding of GI.1 HuNoV were mined from oyster tissues using a bacterial cell surface display system. Fifty-five candidate ligands were identified and selected through mass spectrometry identification and bioinformatics analysis. Among them, the oyster tumor necrosis factor (oTNF) and oyster intraflagellar transport protein (oIFT) showed strong binding abilities with the P protein of GI.1 HuNoV. In addition, the highest mRNA level of these two proteins was found in the digestive glands, which is consistent with GI.1 HuNoV distribution. Overall the findings suggested that oTNF and oIFT may play important roles in the bioaccumulation of GI.1 HuNoV. Text Crassostrea gigas Pacific oyster MDPI Open Access Publishing Pacific Viruses 15 3 631 |
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Open Polar |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
human norovirus Crassostrea gigas specific binding ligands tumor necrosis factor intraflagellar transport protein |
spellingShingle |
human norovirus Crassostrea gigas specific binding ligands tumor necrosis factor intraflagellar transport protein Chenang Lyu Jingwen Li Zhentao Shi Ran An Yanfei Wang Guangda Luo Dapeng Wang Identification of Potential Proteinaceous Ligands of GI.1 Norovirus in Pacific Oyster Tissues |
topic_facet |
human norovirus Crassostrea gigas specific binding ligands tumor necrosis factor intraflagellar transport protein |
description |
Human norovirus (HuNoV) is the leading foodborne pathogen causing nonbacterial gastroenteritis worldwide. The oyster is an important vehicle for HuNoV transmission, especially the GI.1 HuNoV. In our previous study, oyster heat shock protein 70 (oHSP 70) was identified as the first proteinaceous ligand of GII.4 HuNoV in Pacific oysters besides the commonly accepted carbohydrate ligands, a histo-blood group antigens (HBGAs)-like substance. However the mismatch of the distribution pattern between discovered ligands and GI.1 HuNoV suggests that other ligands may exist. In our study, proteinaceous ligands for the specific binding of GI.1 HuNoV were mined from oyster tissues using a bacterial cell surface display system. Fifty-five candidate ligands were identified and selected through mass spectrometry identification and bioinformatics analysis. Among them, the oyster tumor necrosis factor (oTNF) and oyster intraflagellar transport protein (oIFT) showed strong binding abilities with the P protein of GI.1 HuNoV. In addition, the highest mRNA level of these two proteins was found in the digestive glands, which is consistent with GI.1 HuNoV distribution. Overall the findings suggested that oTNF and oIFT may play important roles in the bioaccumulation of GI.1 HuNoV. |
format |
Text |
author |
Chenang Lyu Jingwen Li Zhentao Shi Ran An Yanfei Wang Guangda Luo Dapeng Wang |
author_facet |
Chenang Lyu Jingwen Li Zhentao Shi Ran An Yanfei Wang Guangda Luo Dapeng Wang |
author_sort |
Chenang Lyu |
title |
Identification of Potential Proteinaceous Ligands of GI.1 Norovirus in Pacific Oyster Tissues |
title_short |
Identification of Potential Proteinaceous Ligands of GI.1 Norovirus in Pacific Oyster Tissues |
title_full |
Identification of Potential Proteinaceous Ligands of GI.1 Norovirus in Pacific Oyster Tissues |
title_fullStr |
Identification of Potential Proteinaceous Ligands of GI.1 Norovirus in Pacific Oyster Tissues |
title_full_unstemmed |
Identification of Potential Proteinaceous Ligands of GI.1 Norovirus in Pacific Oyster Tissues |
title_sort |
identification of potential proteinaceous ligands of gi.1 norovirus in pacific oyster tissues |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/v15030631 |
op_coverage |
agris |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Viruses; Volume 15; Issue 3; Pages: 631 |
op_relation |
Human Virology and Viral Diseases https://dx.doi.org/10.3390/v15030631 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/v15030631 |
container_title |
Viruses |
container_volume |
15 |
container_issue |
3 |
container_start_page |
631 |
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1774716980116324352 |