Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels (Anguilla rostrata) in China
Anguillid herpesvirus 1 (AngHV-1) is a pathogen that causes hemorrhagic disease in various farmed and wild freshwater eel species, resulting in significant economic losses. Although AngHV-1 has been detected in the American eel (Anguilla rostrata), its pathogenicity has not been well characterized....
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ftmdpi:oai:mdpi.com:/1999-4915/14/12/2722/ 2023-08-20T04:06:20+02:00 Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels (Anguilla rostrata) in China Rui Guo Zheng Zhang Tianliang He Miaomiao Li Yuchen Zhuo Xiaoqiang Yang Haiping Fan Xinhua Chen agris 2022-12-07 application/pdf https://doi.org/10.3390/v14122722 EN eng Multidisciplinary Digital Publishing Institute Viral Immunology, Vaccines, and Antivirals https://dx.doi.org/10.3390/v14122722 https://creativecommons.org/licenses/by/4.0/ Viruses; Volume 14; Issue 12; Pages: 2722 Anguillid herpesvirus 1 AngHV-1-FC American eel ( Anguilla rostrate ) pathogenicity genomic analysis Text 2022 ftmdpi https://doi.org/10.3390/v14122722 2023-08-01T07:40:57Z Anguillid herpesvirus 1 (AngHV-1) is a pathogen that causes hemorrhagic disease in various farmed and wild freshwater eel species, resulting in significant economic losses. Although AngHV-1 has been detected in the American eel (Anguilla rostrata), its pathogenicity has not been well characterized. In this study, an AngHV-1 isolate, tentatively named AngHV-1-FC, was isolated from diseased American eels with similar symptoms as those observed in AngHV-1-infected European eels and Japanese eels. AngHV-1-FC induced severe cytopathic effects in the European eel spleen cell line (EES), and numerous concentric circular virions were observed in the infected EES cells by transmission electron microscopy. Moreover, AngHV-1-FC caused the same symptoms as the naturally diseased European eels and Japanese eels through experimental infection, resulting in a 100% morbidity rate and 13.3% mortality rate. The whole genome sequence analyses showed that the average nucleotide identity value between AngHV-1-FC and other AngHV-1 isolates ranged from 99.28% to 99.55%. However, phylogenetic analysis revealed that there was a genetic divergence between AngHV-1-FC and other AngHV-1 isolates, suggesting that AngHV-1-FC was a new isolate of AngHV-1. Thus, our results indicated that AngHV-1-FC can infect farmed American eels, with a high pathogenicity, providing new knowledge in regard to the prevalence and prevention of AngHV-1. Text European eel MDPI Open Access Publishing Viruses 14 12 2722 |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
Anguillid herpesvirus 1 AngHV-1-FC American eel ( Anguilla rostrate ) pathogenicity genomic analysis |
spellingShingle |
Anguillid herpesvirus 1 AngHV-1-FC American eel ( Anguilla rostrate ) pathogenicity genomic analysis Rui Guo Zheng Zhang Tianliang He Miaomiao Li Yuchen Zhuo Xiaoqiang Yang Haiping Fan Xinhua Chen Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels (Anguilla rostrata) in China |
topic_facet |
Anguillid herpesvirus 1 AngHV-1-FC American eel ( Anguilla rostrate ) pathogenicity genomic analysis |
description |
Anguillid herpesvirus 1 (AngHV-1) is a pathogen that causes hemorrhagic disease in various farmed and wild freshwater eel species, resulting in significant economic losses. Although AngHV-1 has been detected in the American eel (Anguilla rostrata), its pathogenicity has not been well characterized. In this study, an AngHV-1 isolate, tentatively named AngHV-1-FC, was isolated from diseased American eels with similar symptoms as those observed in AngHV-1-infected European eels and Japanese eels. AngHV-1-FC induced severe cytopathic effects in the European eel spleen cell line (EES), and numerous concentric circular virions were observed in the infected EES cells by transmission electron microscopy. Moreover, AngHV-1-FC caused the same symptoms as the naturally diseased European eels and Japanese eels through experimental infection, resulting in a 100% morbidity rate and 13.3% mortality rate. The whole genome sequence analyses showed that the average nucleotide identity value between AngHV-1-FC and other AngHV-1 isolates ranged from 99.28% to 99.55%. However, phylogenetic analysis revealed that there was a genetic divergence between AngHV-1-FC and other AngHV-1 isolates, suggesting that AngHV-1-FC was a new isolate of AngHV-1. Thus, our results indicated that AngHV-1-FC can infect farmed American eels, with a high pathogenicity, providing new knowledge in regard to the prevalence and prevention of AngHV-1. |
format |
Text |
author |
Rui Guo Zheng Zhang Tianliang He Miaomiao Li Yuchen Zhuo Xiaoqiang Yang Haiping Fan Xinhua Chen |
author_facet |
Rui Guo Zheng Zhang Tianliang He Miaomiao Li Yuchen Zhuo Xiaoqiang Yang Haiping Fan Xinhua Chen |
author_sort |
Rui Guo |
title |
Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels (Anguilla rostrata) in China |
title_short |
Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels (Anguilla rostrata) in China |
title_full |
Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels (Anguilla rostrata) in China |
title_fullStr |
Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels (Anguilla rostrata) in China |
title_full_unstemmed |
Isolation and Identification of a New Isolate of Anguillid Herpesvirus 1 from Farmed American Eels (Anguilla rostrata) in China |
title_sort |
isolation and identification of a new isolate of anguillid herpesvirus 1 from farmed american eels (anguilla rostrata) in china |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/v14122722 |
op_coverage |
agris |
genre |
European eel |
genre_facet |
European eel |
op_source |
Viruses; Volume 14; Issue 12; Pages: 2722 |
op_relation |
Viral Immunology, Vaccines, and Antivirals https://dx.doi.org/10.3390/v14122722 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/v14122722 |
container_title |
Viruses |
container_volume |
14 |
container_issue |
12 |
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2722 |
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1774717336988680192 |