Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain
In Europe, two species of hantaviruses, Puumala orthohantavirus (PUUV) and Dobrava orthohantavirus (DOBV), cause hemorrhagic fever with renal syndrome in humans. The rodent reservoirs for these viruses are common throughout Ukraine, and hence, the goal of this study was to identify the species and s...
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ftmdpi:oai:mdpi.com:/1999-4915/13/8/1640/ 2023-08-20T04:07:59+02:00 Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain Evan P. Williams Mariah K. Taylor Iryna Demchyshyna Igor Nebogatkin Olena Nesterova Iryna Khuda Lyudmyla Chernenko Oleksandra A. Hluzd Vira V. Kutseva Gregory E. Glass Nataliia Yanko Colleen B. Jonsson agris 2021-08-18 application/pdf https://doi.org/10.3390/v13081640 EN eng Multidisciplinary Digital Publishing Institute Animal Viruses https://dx.doi.org/10.3390/v13081640 https://creativecommons.org/licenses/by/4.0/ Viruses; Volume 13; Issue 8; Pages: 1640 Puumala orthohantavirus Ukraine next generation sequencing field survey Text 2021 ftmdpi https://doi.org/10.3390/v13081640 2023-08-01T02:28:17Z In Europe, two species of hantaviruses, Puumala orthohantavirus (PUUV) and Dobrava orthohantavirus (DOBV), cause hemorrhagic fever with renal syndrome in humans. The rodent reservoirs for these viruses are common throughout Ukraine, and hence, the goal of this study was to identify the species and strains of hantaviruses circulating in this region. We conducted surveillance of small rodent populations in a rural region in northwestern Ukraine approximately 30 km from Poland. From the 424 small mammals captured, we identified nine species, of which the most abundant were Myodes glareolus, the bank vole (45%); Apodemus flavicollis, the yellow-necked mouse (29%); and Apodemus agrarius, the striped field mouse (14.6%) Using an indirect immunofluorescence assay, 15.7%, 20.5%, and 33.9% of the sera from M. glareolus, A. glareolus, and A. flavicollis were positive for hantaviral antibodies, respectively. Additionally, we detected antibodies to the hantaviral antigen in one Microtus arvalis, one Mus musculus, and one Sorex minutus. We screened the lung tissue for hantaviral RNA using next-generation sequencing and identified PUUV sequences in 25 small mammals, including 23 M. glareolus, 1 M. musculus, and 1 A. flavicollis, but we were unable to detect DOBV sequences in any of our A. agrarius specimens. The percent identity matrix and Bayesian phylogenetic analyses of the S-segment of PUUV from 14 M. glareolus lungs suggest the highest similarity (92–95% nucleotide or 99–100% amino acid) with the Latvian lineage. This new genetic information will contribute to future molecular surveillance of human cases in Ukraine. Text Microtus arvalis MDPI Open Access Publishing Viruses 13 8 1640 |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
Puumala orthohantavirus Ukraine next generation sequencing field survey |
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Puumala orthohantavirus Ukraine next generation sequencing field survey Evan P. Williams Mariah K. Taylor Iryna Demchyshyna Igor Nebogatkin Olena Nesterova Iryna Khuda Lyudmyla Chernenko Oleksandra A. Hluzd Vira V. Kutseva Gregory E. Glass Nataliia Yanko Colleen B. Jonsson Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain |
topic_facet |
Puumala orthohantavirus Ukraine next generation sequencing field survey |
description |
In Europe, two species of hantaviruses, Puumala orthohantavirus (PUUV) and Dobrava orthohantavirus (DOBV), cause hemorrhagic fever with renal syndrome in humans. The rodent reservoirs for these viruses are common throughout Ukraine, and hence, the goal of this study was to identify the species and strains of hantaviruses circulating in this region. We conducted surveillance of small rodent populations in a rural region in northwestern Ukraine approximately 30 km from Poland. From the 424 small mammals captured, we identified nine species, of which the most abundant were Myodes glareolus, the bank vole (45%); Apodemus flavicollis, the yellow-necked mouse (29%); and Apodemus agrarius, the striped field mouse (14.6%) Using an indirect immunofluorescence assay, 15.7%, 20.5%, and 33.9% of the sera from M. glareolus, A. glareolus, and A. flavicollis were positive for hantaviral antibodies, respectively. Additionally, we detected antibodies to the hantaviral antigen in one Microtus arvalis, one Mus musculus, and one Sorex minutus. We screened the lung tissue for hantaviral RNA using next-generation sequencing and identified PUUV sequences in 25 small mammals, including 23 M. glareolus, 1 M. musculus, and 1 A. flavicollis, but we were unable to detect DOBV sequences in any of our A. agrarius specimens. The percent identity matrix and Bayesian phylogenetic analyses of the S-segment of PUUV from 14 M. glareolus lungs suggest the highest similarity (92–95% nucleotide or 99–100% amino acid) with the Latvian lineage. This new genetic information will contribute to future molecular surveillance of human cases in Ukraine. |
format |
Text |
author |
Evan P. Williams Mariah K. Taylor Iryna Demchyshyna Igor Nebogatkin Olena Nesterova Iryna Khuda Lyudmyla Chernenko Oleksandra A. Hluzd Vira V. Kutseva Gregory E. Glass Nataliia Yanko Colleen B. Jonsson |
author_facet |
Evan P. Williams Mariah K. Taylor Iryna Demchyshyna Igor Nebogatkin Olena Nesterova Iryna Khuda Lyudmyla Chernenko Oleksandra A. Hluzd Vira V. Kutseva Gregory E. Glass Nataliia Yanko Colleen B. Jonsson |
author_sort |
Evan P. Williams |
title |
Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain |
title_short |
Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain |
title_full |
Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain |
title_fullStr |
Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain |
title_full_unstemmed |
Prevalence of Hantaviruses Harbored by Murid Rodents in Northwestern Ukraine and Discovery of a Novel Puumala Virus Strain |
title_sort |
prevalence of hantaviruses harbored by murid rodents in northwestern ukraine and discovery of a novel puumala virus strain |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/v13081640 |
op_coverage |
agris |
genre |
Microtus arvalis |
genre_facet |
Microtus arvalis |
op_source |
Viruses; Volume 13; Issue 8; Pages: 1640 |
op_relation |
Animal Viruses https://dx.doi.org/10.3390/v13081640 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/v13081640 |
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Viruses |
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13 |
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8 |
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1640 |
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