Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity
To date, the microbiome, as well as the virome of the Croatian populations of bats, was unknown. Here, we present the results of the first viral metagenomic analysis of guano, feces and saliva (oral swabs) of seven bat species (Myotis myotis, Miniopterus schreibersii, Rhinolophus ferrumequinum, Epte...
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ftmdpi:oai:mdpi.com:/1999-4915/12/8/891/ 2023-08-20T04:08:00+02:00 Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity Ivana Šimić Tomaž Mark Zorec Ivana Lojkić Nina Krešić Mario Poljak Florence Cliquet Evelyne Picard-Meyer Marine Wasniewski Vida Zrnčić Anđela Ćukušić Tomislav Bedeković agris 2020-08-14 application/pdf https://doi.org/10.3390/v12080891 EN eng Multidisciplinary Digital Publishing Institute Animal Viruses https://dx.doi.org/10.3390/v12080891 https://creativecommons.org/licenses/by/4.0/ Viruses; Volume 12; Issue 8; Pages: 891 bats viral metagenomics Croatia virus diversity Text 2020 ftmdpi https://doi.org/10.3390/v12080891 2023-07-31T23:55:57Z To date, the microbiome, as well as the virome of the Croatian populations of bats, was unknown. Here, we present the results of the first viral metagenomic analysis of guano, feces and saliva (oral swabs) of seven bat species (Myotis myotis, Miniopterus schreibersii, Rhinolophus ferrumequinum, Eptesicus serotinus, Myotis blythii, Myotis nattereri and Myotis emarginatus) conducted in Mediterranean and continental Croatia. Viral nucleic acids were extracted from sample pools, and analyzed using Illumina sequencing. The presence of 63 different viral families representing all seven Baltimore groups were confirmed, most commonly insect viruses likely reflecting the diet of insectivorous bats. Virome compositions of our samples were largely impacted by the sample type: invertebrate-infecting viruses were most frequently found in feces, bacterial viruses in guano, whereas vertebrate-infecting viruses were most common in swabs. Most vertebrate-infecting virus sequences were assigned to retroviruses, parvoviruses, iridoviruses, and poxviruses. We further report the complete genome sequence of a novel adeno-associated virus, densovirus and a near complete length genome sequence of a novel iflavirus. Additionally, one of the most interesting findings in this study was the difference in viromes between two contrasting habitats, the continental and Mediterranean Croatia. Text Myotis nattereri MDPI Open Access Publishing Guano ENVELOPE(141.604,141.604,-66.775,-66.775) Viruses 12 8 891 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
bats viral metagenomics Croatia virus diversity |
spellingShingle |
bats viral metagenomics Croatia virus diversity Ivana Šimić Tomaž Mark Zorec Ivana Lojkić Nina Krešić Mario Poljak Florence Cliquet Evelyne Picard-Meyer Marine Wasniewski Vida Zrnčić Anđela Ćukušić Tomislav Bedeković Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity |
topic_facet |
bats viral metagenomics Croatia virus diversity |
description |
To date, the microbiome, as well as the virome of the Croatian populations of bats, was unknown. Here, we present the results of the first viral metagenomic analysis of guano, feces and saliva (oral swabs) of seven bat species (Myotis myotis, Miniopterus schreibersii, Rhinolophus ferrumequinum, Eptesicus serotinus, Myotis blythii, Myotis nattereri and Myotis emarginatus) conducted in Mediterranean and continental Croatia. Viral nucleic acids were extracted from sample pools, and analyzed using Illumina sequencing. The presence of 63 different viral families representing all seven Baltimore groups were confirmed, most commonly insect viruses likely reflecting the diet of insectivorous bats. Virome compositions of our samples were largely impacted by the sample type: invertebrate-infecting viruses were most frequently found in feces, bacterial viruses in guano, whereas vertebrate-infecting viruses were most common in swabs. Most vertebrate-infecting virus sequences were assigned to retroviruses, parvoviruses, iridoviruses, and poxviruses. We further report the complete genome sequence of a novel adeno-associated virus, densovirus and a near complete length genome sequence of a novel iflavirus. Additionally, one of the most interesting findings in this study was the difference in viromes between two contrasting habitats, the continental and Mediterranean Croatia. |
format |
Text |
author |
Ivana Šimić Tomaž Mark Zorec Ivana Lojkić Nina Krešić Mario Poljak Florence Cliquet Evelyne Picard-Meyer Marine Wasniewski Vida Zrnčić Anđela Ćukušić Tomislav Bedeković |
author_facet |
Ivana Šimić Tomaž Mark Zorec Ivana Lojkić Nina Krešić Mario Poljak Florence Cliquet Evelyne Picard-Meyer Marine Wasniewski Vida Zrnčić Anđela Ćukušić Tomislav Bedeković |
author_sort |
Ivana Šimić |
title |
Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity |
title_short |
Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity |
title_full |
Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity |
title_fullStr |
Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity |
title_full_unstemmed |
Viral Metagenomic Profiling of Croatian Bat Population Reveals Sample and Habitat Dependent Diversity |
title_sort |
viral metagenomic profiling of croatian bat population reveals sample and habitat dependent diversity |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/v12080891 |
op_coverage |
agris |
long_lat |
ENVELOPE(141.604,141.604,-66.775,-66.775) |
geographic |
Guano |
geographic_facet |
Guano |
genre |
Myotis nattereri |
genre_facet |
Myotis nattereri |
op_source |
Viruses; Volume 12; Issue 8; Pages: 891 |
op_relation |
Animal Viruses https://dx.doi.org/10.3390/v12080891 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/v12080891 |
container_title |
Viruses |
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12 |
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8 |
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891 |
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1774720022504013824 |