High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses
Victoria and Yamagata lineages of influenza B viruses are globally circulating in seasonal epidemics. Madin–Darby canine kidney (MDCK) cells are permissive for viral isolation and vaccine manufacture. Nevertheless, the interplay between influenza B viruses and host microRNAs has not been investigate...
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ftmdpi:oai:mdpi.com:/1999-4915/11/11/986/ 2023-08-20T04:05:48+02:00 High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses Suthat Saengchoowong Kritsada Khongnomnan Witthaya Poomipak Kesmanee Praianantathavorn Yong Poovorawan Qibo Zhang Sunchai Payungporn agris 2019-10-25 application/pdf https://doi.org/10.3390/v11110986 EN eng Multidisciplinary Digital Publishing Institute Animal Viruses https://dx.doi.org/10.3390/v11110986 https://creativecommons.org/licenses/by/4.0/ Viruses; Volume 11; Issue 11; Pages: 986 microRNAs influenza B viruses Yamagata Victoria MDCK next-generation sequencing Canis lupus familiaris Text 2019 ftmdpi https://doi.org/10.3390/v11110986 2023-07-31T22:44:03Z Victoria and Yamagata lineages of influenza B viruses are globally circulating in seasonal epidemics. Madin–Darby canine kidney (MDCK) cells are permissive for viral isolation and vaccine manufacture. Nevertheless, the interplay between influenza B viruses and host microRNAs has not been investigated in this cell line. Therefore, the present study aims at high-throughput analysis of canine microRNA profile upon infection of influenza B viruses. Briefly, MDCK cells were infected with Victoria or Yamagata lineage at MOI of 0.01. After being harvested at 6, 12 and 24 h post infection, microRNAs were subjected to high-throughput sequencing based on MiSeq platform (Illumina). The results demonstrated that five microRNAs including cfa-miR-197, cfa-miR-215, cfa-miR361, cfa-miR-1841, and cfa-miR-1842 were overexpressed in both Victoria and Yamagata lineage infections. Interestingly, computational prediction showed that karyopherin alpha 6 (KPNA6) was targeted by cfa-miR-197 and cfa-miR-215. Moreover, the binding sites of both microRNAs were assessed by 3′-UTR reporter assay. The results showed that only cfa-miR-197 could bind to the target sites of KPNA6, leading to suppressing luciferase activity. Additionally, silencing of KPNA6 was confirmed by overexpression of cfa-miR-197. This study provides canine microRNA responses to seasonal influenza B viruses, suggesting that virus-mediated microRNAs might play crucial roles in host gene regulation. Text Canis lupus MDPI Open Access Publishing Darby ENVELOPE(162.217,162.217,-77.667,-77.667) Viruses 11 11 986 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
microRNAs influenza B viruses Yamagata Victoria MDCK next-generation sequencing Canis lupus familiaris |
spellingShingle |
microRNAs influenza B viruses Yamagata Victoria MDCK next-generation sequencing Canis lupus familiaris Suthat Saengchoowong Kritsada Khongnomnan Witthaya Poomipak Kesmanee Praianantathavorn Yong Poovorawan Qibo Zhang Sunchai Payungporn High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses |
topic_facet |
microRNAs influenza B viruses Yamagata Victoria MDCK next-generation sequencing Canis lupus familiaris |
description |
Victoria and Yamagata lineages of influenza B viruses are globally circulating in seasonal epidemics. Madin–Darby canine kidney (MDCK) cells are permissive for viral isolation and vaccine manufacture. Nevertheless, the interplay between influenza B viruses and host microRNAs has not been investigated in this cell line. Therefore, the present study aims at high-throughput analysis of canine microRNA profile upon infection of influenza B viruses. Briefly, MDCK cells were infected with Victoria or Yamagata lineage at MOI of 0.01. After being harvested at 6, 12 and 24 h post infection, microRNAs were subjected to high-throughput sequencing based on MiSeq platform (Illumina). The results demonstrated that five microRNAs including cfa-miR-197, cfa-miR-215, cfa-miR361, cfa-miR-1841, and cfa-miR-1842 were overexpressed in both Victoria and Yamagata lineage infections. Interestingly, computational prediction showed that karyopherin alpha 6 (KPNA6) was targeted by cfa-miR-197 and cfa-miR-215. Moreover, the binding sites of both microRNAs were assessed by 3′-UTR reporter assay. The results showed that only cfa-miR-197 could bind to the target sites of KPNA6, leading to suppressing luciferase activity. Additionally, silencing of KPNA6 was confirmed by overexpression of cfa-miR-197. This study provides canine microRNA responses to seasonal influenza B viruses, suggesting that virus-mediated microRNAs might play crucial roles in host gene regulation. |
format |
Text |
author |
Suthat Saengchoowong Kritsada Khongnomnan Witthaya Poomipak Kesmanee Praianantathavorn Yong Poovorawan Qibo Zhang Sunchai Payungporn |
author_facet |
Suthat Saengchoowong Kritsada Khongnomnan Witthaya Poomipak Kesmanee Praianantathavorn Yong Poovorawan Qibo Zhang Sunchai Payungporn |
author_sort |
Suthat Saengchoowong |
title |
High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses |
title_short |
High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses |
title_full |
High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses |
title_fullStr |
High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses |
title_full_unstemmed |
High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses |
title_sort |
high-throughput microrna profiles of permissive madin-darby canine kidney cell line infected with influenza b viruses |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2019 |
url |
https://doi.org/10.3390/v11110986 |
op_coverage |
agris |
long_lat |
ENVELOPE(162.217,162.217,-77.667,-77.667) |
geographic |
Darby |
geographic_facet |
Darby |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Viruses; Volume 11; Issue 11; Pages: 986 |
op_relation |
Animal Viruses https://dx.doi.org/10.3390/v11110986 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/v11110986 |
container_title |
Viruses |
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11 |
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11 |
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986 |
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1774716547208577024 |