Heterotrophic Microbiota from the Oligotrophic Waters of Lake Vostok, Antarctica

• Published in: International Journal of Environmental Research and Public Health
• Main Authors: Ekaterina Y. Epova, Alexei B. Shevelev, Ramazan M. Akbayev, Yulia K. Biryukova, Marina V. Zylkova, Elena S. Bogdanova, Marina A. Guseva, Yaroslav Y. Tynio, Vladislav V. Egorov
• Format: Text
• Language: English
• Published: Multidisciplinary Digital Publishing Institute 2022
• Subjects: Antarctica; lake; fresh water; microbiota; 16S rDNA; chemoorganotrophs; Microbacterium testaceum; Microbacterium trichothecenolyticum; Brevundimonas diminuta; Sphingomonas oligophenolica; Sphingomonas; Sphingobium limneticum; Dendryphion; Cladosporium fusiforme; Lake Vostok; Antarc*
• Online Access: https://doi.org/10.3390/ijerph19074025

Lake Vostok is the deepest lake of Antarctica but has poor accessibility for study due to a thick glacial cover, however, water samples of this lake have become available for study just recently. Previously, only the microbiome of the ice cover samples was characterized. Here we report results of bacteriological seeding with subsequent identification of the heterotrophic microorganisms (bacteria and micellar fungi) present by 16S rDNA sequencing as well as results of a direct molecular study of the water microbiome. Surprisingly, the data obtained gave evidence of a predominant occurrence of common chemoorganotrophs that were rather psychrotolerant than psychrophilic. We isolated and described strains belonging to eight heterotrophic microbial species able to grow in a rich medium: six bacterial strains belonging to the species Microbacterium testaceum and Microbacterium trichothecenolyticum, Brevundimonas diminuta, Sphingomonas oligophenolica, Sphingomonas sp. and Sphingobium limneticum; and two fungal strains belonging to Dendryphion sp. and Cladosporium fusiforme. Direct study of 16S rDNA purified water samples confirmed the predominance of the Brevundimonas, Microbacterium, Bradyrhizobium, and Bacillus (Bacillus cereus) genera.