Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey
Several microorganisms were isolated from soil/sediment samples of Antarctic Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated the enzyme activity (oxidoreductases) for enantiosele...
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Molecular Diversity Preservation International
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ftmdpi:oai:mdpi.com:/1660-3397/9/5/889/ 2023-08-20T04:02:34+02:00 Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey Lidiane S. Araújo Edna Kagohara Thaís P. Garcia Vivian H. Pellizari Leandro H. Andrade agris 2011-05-24 application/pdf https://doi.org/10.3390/md9050889 EN eng Molecular Diversity Preservation International https://dx.doi.org/10.3390/md9050889 https://creativecommons.org/licenses/by/3.0/ Marine Drugs; Volume 9; Issue 5; Pages: 889-905 oxidoreductases chiral alcohols psychrophile psychrotroph oxidation deracemisation Text 2011 ftmdpi https://doi.org/10.3390/md9050889 2023-07-31T20:26:35Z Several microorganisms were isolated from soil/sediment samples of Antarctic Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated the enzyme activity (oxidoreductases) for enantioselective oxidation reactions, by using derivatives of (RS)-1-(phenyl)ethanol as substrates. Among the studied microorganisms, 15 psychrophile/psychrotroph strains contain oxidoreductases that catalyze the (S)-enantiomer oxidation from racemic alcohols to their corresponding ketones. Among the identified microorganisms, Flavobacterium sp. and Arthrobacter sp. showed excellent enzymatic activity. These new bacteria strains were selected for optimization study, in which the (RS)-1-(4-methyl-phenyl)ethanol oxidation was evaluated in several reaction conditions. From these studies, it was observed that Flavobacterium sp. has an excellent enzymatic activity at 10 °C and Arthrobacter sp. at 15 and 25 °C. We have also determined the growth curves of these bacteria, and both strains showed optimum growth at 25 °C, indicating that these bacteria are psychrotroph. Text Antarc* Antarctic Antarctic Peninsula MDPI Open Access Publishing Antarctic Antarctic Peninsula Marine Drugs 9 5 889 905 |
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MDPI Open Access Publishing |
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English |
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oxidoreductases chiral alcohols psychrophile psychrotroph oxidation deracemisation |
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oxidoreductases chiral alcohols psychrophile psychrotroph oxidation deracemisation Lidiane S. Araújo Edna Kagohara Thaís P. Garcia Vivian H. Pellizari Leandro H. Andrade Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
topic_facet |
oxidoreductases chiral alcohols psychrophile psychrotroph oxidation deracemisation |
description |
Several microorganisms were isolated from soil/sediment samples of Antarctic Peninsula. The enrichment technique using (RS)-1-(phenyl)ethanol as a carbon source allowed us to isolate 232 psychrophile/psychrotroph microorganisms. We also evaluated the enzyme activity (oxidoreductases) for enantioselective oxidation reactions, by using derivatives of (RS)-1-(phenyl)ethanol as substrates. Among the studied microorganisms, 15 psychrophile/psychrotroph strains contain oxidoreductases that catalyze the (S)-enantiomer oxidation from racemic alcohols to their corresponding ketones. Among the identified microorganisms, Flavobacterium sp. and Arthrobacter sp. showed excellent enzymatic activity. These new bacteria strains were selected for optimization study, in which the (RS)-1-(4-methyl-phenyl)ethanol oxidation was evaluated in several reaction conditions. From these studies, it was observed that Flavobacterium sp. has an excellent enzymatic activity at 10 °C and Arthrobacter sp. at 15 and 25 °C. We have also determined the growth curves of these bacteria, and both strains showed optimum growth at 25 °C, indicating that these bacteria are psychrotroph. |
format |
Text |
author |
Lidiane S. Araújo Edna Kagohara Thaís P. Garcia Vivian H. Pellizari Leandro H. Andrade |
author_facet |
Lidiane S. Araújo Edna Kagohara Thaís P. Garcia Vivian H. Pellizari Leandro H. Andrade |
author_sort |
Lidiane S. Araújo |
title |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_short |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_full |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_fullStr |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_full_unstemmed |
Screening of Microorganisms Producing Cold-Active Oxidoreductases to Be Applied in Enantioselective Alcohol Oxidation. An Antarctic Survey |
title_sort |
screening of microorganisms producing cold-active oxidoreductases to be applied in enantioselective alcohol oxidation. an antarctic survey |
publisher |
Molecular Diversity Preservation International |
publishDate |
2011 |
url |
https://doi.org/10.3390/md9050889 |
op_coverage |
agris |
geographic |
Antarctic Antarctic Peninsula |
geographic_facet |
Antarctic Antarctic Peninsula |
genre |
Antarc* Antarctic Antarctic Peninsula |
genre_facet |
Antarc* Antarctic Antarctic Peninsula |
op_source |
Marine Drugs; Volume 9; Issue 5; Pages: 889-905 |
op_relation |
https://dx.doi.org/10.3390/md9050889 |
op_rights |
https://creativecommons.org/licenses/by/3.0/ |
op_doi |
https://doi.org/10.3390/md9050889 |
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Marine Drugs |
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9 |
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5 |
container_start_page |
889 |
op_container_end_page |
905 |
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1774713084629221376 |