Are Known Cyanotoxins Involved in the Toxicity of Picoplanktonic and Filamentous North Atlantic Marine Cyanobacteria?
Eight marine cyanobacteria strains of the genera Cyanobium, Leptolyngbya, Oscillatoria, Phormidium, and Synechococcus were isolated from rocky beaches along the Atlantic Portuguese central coast and tested for ecotoxicity. Strains were identified by morphological characteristics and by the amplifica...
Published in: | Marine Drugs |
---|---|
Main Authors: | , , |
Format: | Text |
Language: | English |
Published: |
Molecular Diversity Preservation International
2010
|
Subjects: | |
Online Access: | https://doi.org/10.3390/md8061908 |
id |
ftmdpi:oai:mdpi.com:/1660-3397/8/6/1908/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/1660-3397/8/6/1908/ 2023-08-20T04:08:23+02:00 Are Known Cyanotoxins Involved in the Toxicity of Picoplanktonic and Filamentous North Atlantic Marine Cyanobacteria? Frazão Martins Vasconcelos agris 2010-06-21 application/pdf https://doi.org/10.3390/md8061908 EN eng Molecular Diversity Preservation International https://dx.doi.org/10.3390/md8061908 https://creativecommons.org/licenses/by/3.0/ Marine Drugs; Volume 8; Issue 6; Pages: 1908-1919 marine cyanobacteria cyanotoxins ecotoxicology Artemia salina toxin genes Text 2010 ftmdpi https://doi.org/10.3390/md8061908 2023-07-31T20:24:53Z Eight marine cyanobacteria strains of the genera Cyanobium, Leptolyngbya, Oscillatoria, Phormidium, and Synechococcus were isolated from rocky beaches along the Atlantic Portuguese central coast and tested for ecotoxicity. Strains were identified by morphological characteristics and by the amplification and sequentiation of the 16S rDNA. Bioactivity of dichloromethane, methanol and aqueous extracts was assessed by the Artemia salina bioassay. Peptide toxin production was screened by matrix assisted laser desorption/ionization time of flight mass spectrometry. Molecular analysis of the genes involved in the production of known cyanotoxins such as microcystins, nodularins and cylindrospermopsin was also performed. Strains were toxic to the brine shrimp A. salina nauplii with aqueous extracts being more toxic than the organic ones. Although mass spectrometry analysis did not reveal the production of microcystins or other known toxic peptides, a positive result for the presence of mcyE gene was found in one Leptolyngbya strain and one Oscillatoria strain. The extensive brine shrimp mortality points to the involvement of other unknown toxins, and the presence of a fragment of genes involved in the cyanotoxin production highlight the potential risk of cyanobacteria occurrence on the Atlantic coast. Text North Atlantic MDPI Open Access Publishing Marine Drugs 8 6 1908 1919 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
marine cyanobacteria cyanotoxins ecotoxicology Artemia salina toxin genes |
spellingShingle |
marine cyanobacteria cyanotoxins ecotoxicology Artemia salina toxin genes Frazão Martins Vasconcelos Are Known Cyanotoxins Involved in the Toxicity of Picoplanktonic and Filamentous North Atlantic Marine Cyanobacteria? |
topic_facet |
marine cyanobacteria cyanotoxins ecotoxicology Artemia salina toxin genes |
description |
Eight marine cyanobacteria strains of the genera Cyanobium, Leptolyngbya, Oscillatoria, Phormidium, and Synechococcus were isolated from rocky beaches along the Atlantic Portuguese central coast and tested for ecotoxicity. Strains were identified by morphological characteristics and by the amplification and sequentiation of the 16S rDNA. Bioactivity of dichloromethane, methanol and aqueous extracts was assessed by the Artemia salina bioassay. Peptide toxin production was screened by matrix assisted laser desorption/ionization time of flight mass spectrometry. Molecular analysis of the genes involved in the production of known cyanotoxins such as microcystins, nodularins and cylindrospermopsin was also performed. Strains were toxic to the brine shrimp A. salina nauplii with aqueous extracts being more toxic than the organic ones. Although mass spectrometry analysis did not reveal the production of microcystins or other known toxic peptides, a positive result for the presence of mcyE gene was found in one Leptolyngbya strain and one Oscillatoria strain. The extensive brine shrimp mortality points to the involvement of other unknown toxins, and the presence of a fragment of genes involved in the cyanotoxin production highlight the potential risk of cyanobacteria occurrence on the Atlantic coast. |
format |
Text |
author |
Frazão Martins Vasconcelos |
author_facet |
Frazão Martins Vasconcelos |
author_sort |
Frazão |
title |
Are Known Cyanotoxins Involved in the Toxicity of Picoplanktonic and Filamentous North Atlantic Marine Cyanobacteria? |
title_short |
Are Known Cyanotoxins Involved in the Toxicity of Picoplanktonic and Filamentous North Atlantic Marine Cyanobacteria? |
title_full |
Are Known Cyanotoxins Involved in the Toxicity of Picoplanktonic and Filamentous North Atlantic Marine Cyanobacteria? |
title_fullStr |
Are Known Cyanotoxins Involved in the Toxicity of Picoplanktonic and Filamentous North Atlantic Marine Cyanobacteria? |
title_full_unstemmed |
Are Known Cyanotoxins Involved in the Toxicity of Picoplanktonic and Filamentous North Atlantic Marine Cyanobacteria? |
title_sort |
are known cyanotoxins involved in the toxicity of picoplanktonic and filamentous north atlantic marine cyanobacteria? |
publisher |
Molecular Diversity Preservation International |
publishDate |
2010 |
url |
https://doi.org/10.3390/md8061908 |
op_coverage |
agris |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_source |
Marine Drugs; Volume 8; Issue 6; Pages: 1908-1919 |
op_relation |
https://dx.doi.org/10.3390/md8061908 |
op_rights |
https://creativecommons.org/licenses/by/3.0/ |
op_doi |
https://doi.org/10.3390/md8061908 |
container_title |
Marine Drugs |
container_volume |
8 |
container_issue |
6 |
container_start_page |
1908 |
op_container_end_page |
1919 |
_version_ |
1774720612993859584 |