Arctic Sea Ice Microalga Chlamydomonas latifrons KNF0041: Identification and Statistical Optimization of Medium for Enhanced Biomass and Omega-3/Omega-6
Polar microorganisms produce biologically active compounds that enable them to survive in harsh environments. These compounds have potential biomedical applications. The green microalga Chlamydomonas latifrons KNF0041, isolated from Arctic sea ice, has been found to produce polyunsaturated fatty aci...
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ftmdpi:oai:mdpi.com:/1660-3397/21/8/454/ 2023-10-01T03:53:51+02:00 Arctic Sea Ice Microalga Chlamydomonas latifrons KNF0041: Identification and Statistical Optimization of Medium for Enhanced Biomass and Omega-3/Omega-6 Hyunsik Chae Sanghee Kim Han-Gu Choi Ji Hee Kim Se Jong Han Eun Jae Kim agris 2023-08-17 application/pdf https://doi.org/10.3390/md21080454 eng eng Multidisciplinary Digital Publishing Institute Marine Biotechnology Related to Drug Discovery or Production https://dx.doi.org/10.3390/md21080454 https://creativecommons.org/licenses/by/4.0/ Marine Drugs Volume 21 Issue 8 Pages: 454 Chlamydomonas latifrons identification medium optimization biomass enhancement Text 2023 ftmdpi https://doi.org/10.3390/md21080454 2023-09-03T23:54:47Z Polar microorganisms produce biologically active compounds that enable them to survive in harsh environments. These compounds have potential biomedical applications. The green microalga Chlamydomonas latifrons KNF0041, isolated from Arctic sea ice, has been found to produce polyunsaturated fatty acids (PUFAs), including omega-3 and omega-6, which have antioxidant properties. To improve the biomass production of strain KNF0041, statistical methods such as the Plackett–Burman design, Box–Behnken design, and response surface methodology (RSM) were utilized for medium optimization. The optimized medium was designed with increased potassium phosphate content and reduced acetic acid (AcOH) content. The use of the optimized medium resulted in an increase in the cell number as biomass of strain KNF0041 by 34.18% and the omega-3 and omega-6 fatty acid (FA) content by 10.04% and 58.29%, respectively, compared to that in normal TAP medium, which is known as the growth medium for Chlamydomonas culture. In this study, Chlamydomonaslatifrons was discovered for the first time in the polar region and identified using morphology and molecular phylogenetic analyses, the secondary structures of the internal transcribed spacers, and optimized culture conditions. The results of this study provide an efficient method for the application of polar microalgae for the production of bioactive compounds. Text Arctic Sea ice MDPI Open Access Publishing Arctic Marine Drugs 21 8 454 |
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Open Polar |
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MDPI Open Access Publishing |
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English |
topic |
Chlamydomonas latifrons identification medium optimization biomass enhancement |
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Chlamydomonas latifrons identification medium optimization biomass enhancement Hyunsik Chae Sanghee Kim Han-Gu Choi Ji Hee Kim Se Jong Han Eun Jae Kim Arctic Sea Ice Microalga Chlamydomonas latifrons KNF0041: Identification and Statistical Optimization of Medium for Enhanced Biomass and Omega-3/Omega-6 |
topic_facet |
Chlamydomonas latifrons identification medium optimization biomass enhancement |
description |
Polar microorganisms produce biologically active compounds that enable them to survive in harsh environments. These compounds have potential biomedical applications. The green microalga Chlamydomonas latifrons KNF0041, isolated from Arctic sea ice, has been found to produce polyunsaturated fatty acids (PUFAs), including omega-3 and omega-6, which have antioxidant properties. To improve the biomass production of strain KNF0041, statistical methods such as the Plackett–Burman design, Box–Behnken design, and response surface methodology (RSM) were utilized for medium optimization. The optimized medium was designed with increased potassium phosphate content and reduced acetic acid (AcOH) content. The use of the optimized medium resulted in an increase in the cell number as biomass of strain KNF0041 by 34.18% and the omega-3 and omega-6 fatty acid (FA) content by 10.04% and 58.29%, respectively, compared to that in normal TAP medium, which is known as the growth medium for Chlamydomonas culture. In this study, Chlamydomonaslatifrons was discovered for the first time in the polar region and identified using morphology and molecular phylogenetic analyses, the secondary structures of the internal transcribed spacers, and optimized culture conditions. The results of this study provide an efficient method for the application of polar microalgae for the production of bioactive compounds. |
format |
Text |
author |
Hyunsik Chae Sanghee Kim Han-Gu Choi Ji Hee Kim Se Jong Han Eun Jae Kim |
author_facet |
Hyunsik Chae Sanghee Kim Han-Gu Choi Ji Hee Kim Se Jong Han Eun Jae Kim |
author_sort |
Hyunsik Chae |
title |
Arctic Sea Ice Microalga Chlamydomonas latifrons KNF0041: Identification and Statistical Optimization of Medium for Enhanced Biomass and Omega-3/Omega-6 |
title_short |
Arctic Sea Ice Microalga Chlamydomonas latifrons KNF0041: Identification and Statistical Optimization of Medium for Enhanced Biomass and Omega-3/Omega-6 |
title_full |
Arctic Sea Ice Microalga Chlamydomonas latifrons KNF0041: Identification and Statistical Optimization of Medium for Enhanced Biomass and Omega-3/Omega-6 |
title_fullStr |
Arctic Sea Ice Microalga Chlamydomonas latifrons KNF0041: Identification and Statistical Optimization of Medium for Enhanced Biomass and Omega-3/Omega-6 |
title_full_unstemmed |
Arctic Sea Ice Microalga Chlamydomonas latifrons KNF0041: Identification and Statistical Optimization of Medium for Enhanced Biomass and Omega-3/Omega-6 |
title_sort |
arctic sea ice microalga chlamydomonas latifrons knf0041: identification and statistical optimization of medium for enhanced biomass and omega-3/omega-6 |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/md21080454 |
op_coverage |
agris |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Sea ice |
genre_facet |
Arctic Sea ice |
op_source |
Marine Drugs Volume 21 Issue 8 Pages: 454 |
op_relation |
Marine Biotechnology Related to Drug Discovery or Production https://dx.doi.org/10.3390/md21080454 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/md21080454 |
container_title |
Marine Drugs |
container_volume |
21 |
container_issue |
8 |
container_start_page |
454 |
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1778520870329778176 |