Two-Step Enzymolysis of Antarctic Krill for Simultaneous Preparation of Value-Added Oil and Enzymolysate
Antarctic krill is a crucial marine resource containing plenty of high-valued nutrients. However, krill oil as a single product has been developed by the current solvent extraction with high cost. From the perspective of comprehensive utilization of Antarctic krill, this study proposed a novel two-s...
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Multidisciplinary Digital Publishing Institute
2023
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ftmdpi:oai:mdpi.com:/1660-3397/21/1/47/ 2023-08-20T04:00:58+02:00 Two-Step Enzymolysis of Antarctic Krill for Simultaneous Preparation of Value-Added Oil and Enzymolysate Xin-Nan Teng Shu-Chang Wang Liaqat Zeb Yue-Sheng Dong Zhi-Long Xiu agris 2023-01-11 application/pdf https://doi.org/10.3390/md21010047 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md21010047 https://creativecommons.org/licenses/by/4.0/ Marine Drugs; Volume 21; Issue 1; Pages: 47 Antarctic krill oil enzymolysis protease chitinase extraction Text 2023 ftmdpi https://doi.org/10.3390/md21010047 2023-08-01T08:14:40Z Antarctic krill is a crucial marine resource containing plenty of high-valued nutrients. However, krill oil as a single product has been developed by the current solvent extraction with high cost. From the perspective of comprehensive utilization of Antarctic krill, this study proposed a novel two-step enzymolysis-assisted extraction in attempt to produce value-added oil and enzymolysate simultaneously. After two-step chitinase/protease hydrolysis, the lipid yield increased from 2.09% to 4.18%, reaching 112% of Soxhlet extraction. The method greatly improved the yields of main components while reducing the impurity content without further refining. After optimization, the oil contained 246.05 mg/g of phospholipid, 80.96 mg/g of free eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and 0.82 mg/g of astaxanthin. The by-product enzymolysate was abundant in water-soluble proteins (34.35 mg/g), oligopeptides (13.92 mg/g), amino acids (34.24 mg/g), and carbohydrates (5.79 mg/g), which was a good source of functional nutrients. In addition, both oil and enzymolysate showed high antioxidant capacity. This novel method could simultaneously provide oil and enzymolysate amounting for 58.61% of dried krill. Text Antarc* Antarctic Antarctic Krill MDPI Open Access Publishing Antarctic Marine Drugs 21 1 47 |
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MDPI Open Access Publishing |
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English |
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Antarctic krill oil enzymolysis protease chitinase extraction |
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Antarctic krill oil enzymolysis protease chitinase extraction Xin-Nan Teng Shu-Chang Wang Liaqat Zeb Yue-Sheng Dong Zhi-Long Xiu Two-Step Enzymolysis of Antarctic Krill for Simultaneous Preparation of Value-Added Oil and Enzymolysate |
topic_facet |
Antarctic krill oil enzymolysis protease chitinase extraction |
description |
Antarctic krill is a crucial marine resource containing plenty of high-valued nutrients. However, krill oil as a single product has been developed by the current solvent extraction with high cost. From the perspective of comprehensive utilization of Antarctic krill, this study proposed a novel two-step enzymolysis-assisted extraction in attempt to produce value-added oil and enzymolysate simultaneously. After two-step chitinase/protease hydrolysis, the lipid yield increased from 2.09% to 4.18%, reaching 112% of Soxhlet extraction. The method greatly improved the yields of main components while reducing the impurity content without further refining. After optimization, the oil contained 246.05 mg/g of phospholipid, 80.96 mg/g of free eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), and 0.82 mg/g of astaxanthin. The by-product enzymolysate was abundant in water-soluble proteins (34.35 mg/g), oligopeptides (13.92 mg/g), amino acids (34.24 mg/g), and carbohydrates (5.79 mg/g), which was a good source of functional nutrients. In addition, both oil and enzymolysate showed high antioxidant capacity. This novel method could simultaneously provide oil and enzymolysate amounting for 58.61% of dried krill. |
format |
Text |
author |
Xin-Nan Teng Shu-Chang Wang Liaqat Zeb Yue-Sheng Dong Zhi-Long Xiu |
author_facet |
Xin-Nan Teng Shu-Chang Wang Liaqat Zeb Yue-Sheng Dong Zhi-Long Xiu |
author_sort |
Xin-Nan Teng |
title |
Two-Step Enzymolysis of Antarctic Krill for Simultaneous Preparation of Value-Added Oil and Enzymolysate |
title_short |
Two-Step Enzymolysis of Antarctic Krill for Simultaneous Preparation of Value-Added Oil and Enzymolysate |
title_full |
Two-Step Enzymolysis of Antarctic Krill for Simultaneous Preparation of Value-Added Oil and Enzymolysate |
title_fullStr |
Two-Step Enzymolysis of Antarctic Krill for Simultaneous Preparation of Value-Added Oil and Enzymolysate |
title_full_unstemmed |
Two-Step Enzymolysis of Antarctic Krill for Simultaneous Preparation of Value-Added Oil and Enzymolysate |
title_sort |
two-step enzymolysis of antarctic krill for simultaneous preparation of value-added oil and enzymolysate |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023 |
url |
https://doi.org/10.3390/md21010047 |
op_coverage |
agris |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctic Krill |
genre_facet |
Antarc* Antarctic Antarctic Krill |
op_source |
Marine Drugs; Volume 21; Issue 1; Pages: 47 |
op_relation |
https://dx.doi.org/10.3390/md21010047 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/md21010047 |
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Marine Drugs |
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21 |
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1 |
container_start_page |
47 |
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1774721702933037056 |