Valorization of Fish Waste: Isolation and Characterization of Acid- and Pepsin-Soluble Collagen from the Scales of Mediterranean Fish and Fabrication of Collagen-Based Nanofibrous Scaffolds
In search of alternative and sustainable sources of collagenous materials for biomedical applications, the scales of five Mediterranean fish species—fished in high tonnage in the Mediterranean region since they represent popular choices for the local diet—as well as those of the Atlantic salmon for...
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ftmdpi:oai:mdpi.com:/1660-3397/20/11/664/ 2023-08-20T04:05:20+02:00 Valorization of Fish Waste: Isolation and Characterization of Acid- and Pepsin-Soluble Collagen from the Scales of Mediterranean Fish and Fabrication of Collagen-Based Nanofibrous Scaffolds Leto-Aikaterini Tziveleka Stefanos Kikionis Labros Karkatzoulis Kostas Bethanis Vassilios Roussis Efstathia Ioannou agris 2022-10-25 application/pdf https://doi.org/10.3390/md20110664 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md20110664 https://creativecommons.org/licenses/by/4.0/ Marine Drugs; Volume 20; Issue 11; Pages: 664 fish scales marine collagen acid-soluble collagen pepsin-soluble collagen nanofibers Text 2022 ftmdpi https://doi.org/10.3390/md20110664 2023-08-01T07:02:08Z In search of alternative and sustainable sources of collagenous materials for biomedical applications, the scales of five Mediterranean fish species—fished in high tonnage in the Mediterranean region since they represent popular choices for the local diet—as well as those of the Atlantic salmon for comparison purposes, were comparatively studied for their acid- and pepsin-soluble collagen content. Fish scales that currently represent a discarded biomass of no value could be efficiently exploited for the production of a high added-value biomaterial. The isolated collagenous materials, which showed the typical electrophoretic patterns of type I collagen, were morphologically and physicochemically characterized. Using scanning electron microscopy the fibrous morphology of the isolated collagens was confirmed, while the hydroxyproline content, in conjunction with infrared spectroscopy and X-ray diffraction studies verified the characteristic for collagen amino acid profile and its secondary structure. The acid- and pepsin-soluble collagens isolated from the fish scales were blended with the bioactive sulfated marine polysaccharide ulvan and polyethylene oxide and electrospun to afford nanofibrous scaffolds that could find applications in the biomedical sector. Text Atlantic salmon MDPI Open Access Publishing Ulvan ENVELOPE(9.033,9.033,63.667,63.667) Marine Drugs 20 11 664 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
fish scales marine collagen acid-soluble collagen pepsin-soluble collagen nanofibers |
spellingShingle |
fish scales marine collagen acid-soluble collagen pepsin-soluble collagen nanofibers Leto-Aikaterini Tziveleka Stefanos Kikionis Labros Karkatzoulis Kostas Bethanis Vassilios Roussis Efstathia Ioannou Valorization of Fish Waste: Isolation and Characterization of Acid- and Pepsin-Soluble Collagen from the Scales of Mediterranean Fish and Fabrication of Collagen-Based Nanofibrous Scaffolds |
topic_facet |
fish scales marine collagen acid-soluble collagen pepsin-soluble collagen nanofibers |
description |
In search of alternative and sustainable sources of collagenous materials for biomedical applications, the scales of five Mediterranean fish species—fished in high tonnage in the Mediterranean region since they represent popular choices for the local diet—as well as those of the Atlantic salmon for comparison purposes, were comparatively studied for their acid- and pepsin-soluble collagen content. Fish scales that currently represent a discarded biomass of no value could be efficiently exploited for the production of a high added-value biomaterial. The isolated collagenous materials, which showed the typical electrophoretic patterns of type I collagen, were morphologically and physicochemically characterized. Using scanning electron microscopy the fibrous morphology of the isolated collagens was confirmed, while the hydroxyproline content, in conjunction with infrared spectroscopy and X-ray diffraction studies verified the characteristic for collagen amino acid profile and its secondary structure. The acid- and pepsin-soluble collagens isolated from the fish scales were blended with the bioactive sulfated marine polysaccharide ulvan and polyethylene oxide and electrospun to afford nanofibrous scaffolds that could find applications in the biomedical sector. |
format |
Text |
author |
Leto-Aikaterini Tziveleka Stefanos Kikionis Labros Karkatzoulis Kostas Bethanis Vassilios Roussis Efstathia Ioannou |
author_facet |
Leto-Aikaterini Tziveleka Stefanos Kikionis Labros Karkatzoulis Kostas Bethanis Vassilios Roussis Efstathia Ioannou |
author_sort |
Leto-Aikaterini Tziveleka |
title |
Valorization of Fish Waste: Isolation and Characterization of Acid- and Pepsin-Soluble Collagen from the Scales of Mediterranean Fish and Fabrication of Collagen-Based Nanofibrous Scaffolds |
title_short |
Valorization of Fish Waste: Isolation and Characterization of Acid- and Pepsin-Soluble Collagen from the Scales of Mediterranean Fish and Fabrication of Collagen-Based Nanofibrous Scaffolds |
title_full |
Valorization of Fish Waste: Isolation and Characterization of Acid- and Pepsin-Soluble Collagen from the Scales of Mediterranean Fish and Fabrication of Collagen-Based Nanofibrous Scaffolds |
title_fullStr |
Valorization of Fish Waste: Isolation and Characterization of Acid- and Pepsin-Soluble Collagen from the Scales of Mediterranean Fish and Fabrication of Collagen-Based Nanofibrous Scaffolds |
title_full_unstemmed |
Valorization of Fish Waste: Isolation and Characterization of Acid- and Pepsin-Soluble Collagen from the Scales of Mediterranean Fish and Fabrication of Collagen-Based Nanofibrous Scaffolds |
title_sort |
valorization of fish waste: isolation and characterization of acid- and pepsin-soluble collagen from the scales of mediterranean fish and fabrication of collagen-based nanofibrous scaffolds |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022 |
url |
https://doi.org/10.3390/md20110664 |
op_coverage |
agris |
long_lat |
ENVELOPE(9.033,9.033,63.667,63.667) |
geographic |
Ulvan |
geographic_facet |
Ulvan |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Marine Drugs; Volume 20; Issue 11; Pages: 664 |
op_relation |
https://dx.doi.org/10.3390/md20110664 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/md20110664 |
container_title |
Marine Drugs |
container_volume |
20 |
container_issue |
11 |
container_start_page |
664 |
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1774715828971765760 |