Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides
The neuropeptides involved in the regulation of reproduction in the Pacific oyster (Crassostrea gigas) are quite diverse. To investigate this diversity, a transcriptomic survey of the visceral ganglia (VG) was carried out over an annual reproductive cycle. RNA-seq data from 26 samples corresponding...
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ftmdpi:oai:mdpi.com:/1660-3397/19/8/452/ 2023-08-20T04:06:02+02:00 Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides Emilie Réalis-Doyelle Julie Schwartz Cédric Cabau Lorane Le Franc Benoit Bernay Guillaume Rivière Christophe Klopp Pascal Favrel agris 2021-08-07 application/pdf https://doi.org/10.3390/md19080452 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md19080452 https://creativecommons.org/licenses/by/4.0/ Marine Drugs; Volume 19; Issue 8; Pages: 452 nervous system transcriptome neuropeptides reproduction Text 2021 ftmdpi https://doi.org/10.3390/md19080452 2023-08-01T02:23:08Z The neuropeptides involved in the regulation of reproduction in the Pacific oyster (Crassostrea gigas) are quite diverse. To investigate this diversity, a transcriptomic survey of the visceral ganglia (VG) was carried out over an annual reproductive cycle. RNA-seq data from 26 samples corresponding to VG at different stages of reproduction were de novo assembled to generate a specific reference transcriptome of the oyster nervous system and used to identify differentially expressed transcripts. Transcriptome mining led to the identification of novel neuropeptide precursors (NPPs) related to the bilaterian Eclosion Hormone (EH), crustacean female sex hormone/Interleukin 17, Nesfatin, neuroparsin/IGFBP, prokineticins, and urotensin I; to the protostome GNQQN, pleurin, prohormones 3 and 4, prothoracotropic hormones (PTTH), and QSamide/PXXXamide; to the lophotrochozoan CCWamide, CLCCY, HFAamide, and LXRX; and to the mollusk-specific NPPs CCCGS, clionin, FYFY, GNamide, GRWRN, GSWN, GWE, IWMPxxGYxx, LXRYamide, RTLFamide, SLRFamide, and WGAGamide. Among the complete repertoire of NPPs, no sex-biased expression was observed. However, 25 NPPs displayed reproduction stage-specific expression, supporting their involvement in the control of gametogenesis or associated metabolisms. Text Crassostrea gigas Pacific oyster MDPI Open Access Publishing Pacific Marine Drugs 19 8 452 |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
nervous system transcriptome neuropeptides reproduction |
spellingShingle |
nervous system transcriptome neuropeptides reproduction Emilie Réalis-Doyelle Julie Schwartz Cédric Cabau Lorane Le Franc Benoit Bernay Guillaume Rivière Christophe Klopp Pascal Favrel Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
topic_facet |
nervous system transcriptome neuropeptides reproduction |
description |
The neuropeptides involved in the regulation of reproduction in the Pacific oyster (Crassostrea gigas) are quite diverse. To investigate this diversity, a transcriptomic survey of the visceral ganglia (VG) was carried out over an annual reproductive cycle. RNA-seq data from 26 samples corresponding to VG at different stages of reproduction were de novo assembled to generate a specific reference transcriptome of the oyster nervous system and used to identify differentially expressed transcripts. Transcriptome mining led to the identification of novel neuropeptide precursors (NPPs) related to the bilaterian Eclosion Hormone (EH), crustacean female sex hormone/Interleukin 17, Nesfatin, neuroparsin/IGFBP, prokineticins, and urotensin I; to the protostome GNQQN, pleurin, prohormones 3 and 4, prothoracotropic hormones (PTTH), and QSamide/PXXXamide; to the lophotrochozoan CCWamide, CLCCY, HFAamide, and LXRX; and to the mollusk-specific NPPs CCCGS, clionin, FYFY, GNamide, GRWRN, GSWN, GWE, IWMPxxGYxx, LXRYamide, RTLFamide, SLRFamide, and WGAGamide. Among the complete repertoire of NPPs, no sex-biased expression was observed. However, 25 NPPs displayed reproduction stage-specific expression, supporting their involvement in the control of gametogenesis or associated metabolisms. |
format |
Text |
author |
Emilie Réalis-Doyelle Julie Schwartz Cédric Cabau Lorane Le Franc Benoit Bernay Guillaume Rivière Christophe Klopp Pascal Favrel |
author_facet |
Emilie Réalis-Doyelle Julie Schwartz Cédric Cabau Lorane Le Franc Benoit Bernay Guillaume Rivière Christophe Klopp Pascal Favrel |
author_sort |
Emilie Réalis-Doyelle |
title |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_short |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_full |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_fullStr |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_full_unstemmed |
Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides |
title_sort |
transcriptome profiling of the pacific oyster crassostrea gigas visceral ganglia over a reproduction cycle identifies novel regulatory peptides |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/md19080452 |
op_coverage |
agris |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Marine Drugs; Volume 19; Issue 8; Pages: 452 |
op_relation |
https://dx.doi.org/10.3390/md19080452 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/md19080452 |
container_title |
Marine Drugs |
container_volume |
19 |
container_issue |
8 |
container_start_page |
452 |
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