Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents
In the last decade, metal nanoparticles (NPs) have gained significant interest in the field of biotechnology due to their unique physiochemical properties and potential uses in a wide range of applications. Metal NP synthesis using microorganisms has emerged as an eco-friendly, clean, and viable str...
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ftmdpi:oai:mdpi.com:/1660-3397/19/5/263/ 2023-08-20T04:00:23+02:00 Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents Maria Sindhura John Joseph Amruthraj Nagoth Marco Zannotti Rita Giovannetti Alessio Mancini Kesava Priyan Ramasamy Cristina Miceli Sandra Pucciarelli agris 2021-05-08 application/pdf https://doi.org/10.3390/md19050263 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md19050263 https://creativecommons.org/licenses/by/4.0/ Marine Drugs; Volume 19; Issue 5; Pages: 263 green synthesis biomaterials metal antibiotics nanotechnology Text 2021 ftmdpi https://doi.org/10.3390/md19050263 2023-08-01T01:40:20Z In the last decade, metal nanoparticles (NPs) have gained significant interest in the field of biotechnology due to their unique physiochemical properties and potential uses in a wide range of applications. Metal NP synthesis using microorganisms has emerged as an eco-friendly, clean, and viable strategy alternative to chemical and physical approaches. Herein, an original and efficient route for the microbial synthesis of copper NPs using bacterial strains newly isolated from an Antarctic consortium is described. UV-visible spectra of the NPs showed a maximum absorbance in the range of 380–385 nm. Transmission electron microscopy analysis showed that these NPs are all monodispersed, spherical in nature, and well segregated without any agglomeration and with an average size of 30 nm. X-ray powder diffraction showed a polycrystalline nature and face centered cubic lattice and revealed characteristic diffraction peaks indicating the formation of CuONPs. Fourier-transform infrared spectra confirmed the presence of capping proteins on the NP surface that act as stabilizers. All CuONPs manifested antimicrobial activity against various types of Gram-negative; Gram-positive bacteria; and fungi pathogen microorganisms including Escherichia coli, Staphylococcus aureus, and Candida albicans. The cost-effective and eco-friendly biosynthesis of these CuONPs make them particularly attractive in several application from nanotechnology to biomedical science. Text Antarc* Antarctic MDPI Open Access Publishing Antarctic Marine Drugs 19 5 263 |
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MDPI Open Access Publishing |
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ftmdpi |
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English |
topic |
green synthesis biomaterials metal antibiotics nanotechnology |
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green synthesis biomaterials metal antibiotics nanotechnology Maria Sindhura John Joseph Amruthraj Nagoth Marco Zannotti Rita Giovannetti Alessio Mancini Kesava Priyan Ramasamy Cristina Miceli Sandra Pucciarelli Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents |
topic_facet |
green synthesis biomaterials metal antibiotics nanotechnology |
description |
In the last decade, metal nanoparticles (NPs) have gained significant interest in the field of biotechnology due to their unique physiochemical properties and potential uses in a wide range of applications. Metal NP synthesis using microorganisms has emerged as an eco-friendly, clean, and viable strategy alternative to chemical and physical approaches. Herein, an original and efficient route for the microbial synthesis of copper NPs using bacterial strains newly isolated from an Antarctic consortium is described. UV-visible spectra of the NPs showed a maximum absorbance in the range of 380–385 nm. Transmission electron microscopy analysis showed that these NPs are all monodispersed, spherical in nature, and well segregated without any agglomeration and with an average size of 30 nm. X-ray powder diffraction showed a polycrystalline nature and face centered cubic lattice and revealed characteristic diffraction peaks indicating the formation of CuONPs. Fourier-transform infrared spectra confirmed the presence of capping proteins on the NP surface that act as stabilizers. All CuONPs manifested antimicrobial activity against various types of Gram-negative; Gram-positive bacteria; and fungi pathogen microorganisms including Escherichia coli, Staphylococcus aureus, and Candida albicans. The cost-effective and eco-friendly biosynthesis of these CuONPs make them particularly attractive in several application from nanotechnology to biomedical science. |
format |
Text |
author |
Maria Sindhura John Joseph Amruthraj Nagoth Marco Zannotti Rita Giovannetti Alessio Mancini Kesava Priyan Ramasamy Cristina Miceli Sandra Pucciarelli |
author_facet |
Maria Sindhura John Joseph Amruthraj Nagoth Marco Zannotti Rita Giovannetti Alessio Mancini Kesava Priyan Ramasamy Cristina Miceli Sandra Pucciarelli |
author_sort |
Maria Sindhura John |
title |
Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents |
title_short |
Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents |
title_full |
Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents |
title_fullStr |
Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents |
title_full_unstemmed |
Biogenic Synthesis of Copper Nanoparticles Using Bacterial Strains Isolated from an Antarctic Consortium Associated to a Psychrophilic Marine Ciliate: Characterization and Potential Application as Antimicrobial Agents |
title_sort |
biogenic synthesis of copper nanoparticles using bacterial strains isolated from an antarctic consortium associated to a psychrophilic marine ciliate: characterization and potential application as antimicrobial agents |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/md19050263 |
op_coverage |
agris |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Marine Drugs; Volume 19; Issue 5; Pages: 263 |
op_relation |
https://dx.doi.org/10.3390/md19050263 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/md19050263 |
container_title |
Marine Drugs |
container_volume |
19 |
container_issue |
5 |
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263 |
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1774717859240345600 |