Hidden Diversity in an Antarctic Algal Forest: Metabolomic Profiling Linked to Patterns of Genetic Diversification in the Antarctic Red Alga Plocamium sp.
The common Antarctic red alga Plocamium sp. is rich in halogenated monoterpenes with known anticancer and antimicrobial properties and extracts of Plocamium sp. have strong ecological activity in deterring feeding by sympatric herbivores. Plocamium sp. collected near Anvers Island, Antarctica showed...
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ftmdpi:oai:mdpi.com:/1660-3397/19/11/607/ 2023-08-20T04:00:23+02:00 Hidden Diversity in an Antarctic Algal Forest: Metabolomic Profiling Linked to Patterns of Genetic Diversification in the Antarctic Red Alga Plocamium sp. Andrew J. Shilling Sabrina Heiser Charles D. Amsler James B. McClintock Bill J. Baker agris 2021-10-27 application/pdf https://doi.org/10.3390/md19110607 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md19110607 https://creativecommons.org/licenses/by/4.0/ Marine Drugs; Volume 19; Issue 11; Pages: 607 halogenated monoterpenes metabolite diversity haplotype diversity Plocamium Antarctica Text 2021 ftmdpi https://doi.org/10.3390/md19110607 2023-08-01T03:04:26Z The common Antarctic red alga Plocamium sp. is rich in halogenated monoterpenes with known anticancer and antimicrobial properties and extracts of Plocamium sp. have strong ecological activity in deterring feeding by sympatric herbivores. Plocamium sp. collected near Anvers Island, Antarctica showed a high degree of secondary metabolite diversity between separate individuals. GC/MS results revealed 15 different combinations of metabolites (chemogroups) across individuals, which were apparent at 50% or greater Bray–Curtis similarity and also clearly distinguishable by eye when comparing chromatographic profiles of the secondary metabolomes. Sequencing of the mitochondrial cox1 gene revealed six distinct haplotypes, of which the most common two had been previously reported (now referred to as Haplotypes 1 and 2). With the exception of one individual, three of the chemogroups were only produced by individuals in Haplotype 1. All the other 12 chemogroups were produced by individuals in Haplotype 2, with five of these chemogroups also present in one of the four new, less common haplotypes that only differed from Haplotype 2 by one base pair. The functional relevance of this metabolomic and genetic diversity is unknown, but they could have important ecological and evolutionary ramifications, thus potentially providing a foundation for differential selection. Text Antarc* Antarctic Antarctica Anvers Island MDPI Open Access Publishing Antarctic Anvers ENVELOPE(-63.500,-63.500,-64.600,-64.600) Anvers Island ENVELOPE(-63.500,-63.500,-64.600,-64.600) Bray ENVELOPE(-114.067,-114.067,-74.833,-74.833) The Antarctic Marine Drugs 19 11 607 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
halogenated monoterpenes metabolite diversity haplotype diversity Plocamium Antarctica |
spellingShingle |
halogenated monoterpenes metabolite diversity haplotype diversity Plocamium Antarctica Andrew J. Shilling Sabrina Heiser Charles D. Amsler James B. McClintock Bill J. Baker Hidden Diversity in an Antarctic Algal Forest: Metabolomic Profiling Linked to Patterns of Genetic Diversification in the Antarctic Red Alga Plocamium sp. |
topic_facet |
halogenated monoterpenes metabolite diversity haplotype diversity Plocamium Antarctica |
description |
The common Antarctic red alga Plocamium sp. is rich in halogenated monoterpenes with known anticancer and antimicrobial properties and extracts of Plocamium sp. have strong ecological activity in deterring feeding by sympatric herbivores. Plocamium sp. collected near Anvers Island, Antarctica showed a high degree of secondary metabolite diversity between separate individuals. GC/MS results revealed 15 different combinations of metabolites (chemogroups) across individuals, which were apparent at 50% or greater Bray–Curtis similarity and also clearly distinguishable by eye when comparing chromatographic profiles of the secondary metabolomes. Sequencing of the mitochondrial cox1 gene revealed six distinct haplotypes, of which the most common two had been previously reported (now referred to as Haplotypes 1 and 2). With the exception of one individual, three of the chemogroups were only produced by individuals in Haplotype 1. All the other 12 chemogroups were produced by individuals in Haplotype 2, with five of these chemogroups also present in one of the four new, less common haplotypes that only differed from Haplotype 2 by one base pair. The functional relevance of this metabolomic and genetic diversity is unknown, but they could have important ecological and evolutionary ramifications, thus potentially providing a foundation for differential selection. |
format |
Text |
author |
Andrew J. Shilling Sabrina Heiser Charles D. Amsler James B. McClintock Bill J. Baker |
author_facet |
Andrew J. Shilling Sabrina Heiser Charles D. Amsler James B. McClintock Bill J. Baker |
author_sort |
Andrew J. Shilling |
title |
Hidden Diversity in an Antarctic Algal Forest: Metabolomic Profiling Linked to Patterns of Genetic Diversification in the Antarctic Red Alga Plocamium sp. |
title_short |
Hidden Diversity in an Antarctic Algal Forest: Metabolomic Profiling Linked to Patterns of Genetic Diversification in the Antarctic Red Alga Plocamium sp. |
title_full |
Hidden Diversity in an Antarctic Algal Forest: Metabolomic Profiling Linked to Patterns of Genetic Diversification in the Antarctic Red Alga Plocamium sp. |
title_fullStr |
Hidden Diversity in an Antarctic Algal Forest: Metabolomic Profiling Linked to Patterns of Genetic Diversification in the Antarctic Red Alga Plocamium sp. |
title_full_unstemmed |
Hidden Diversity in an Antarctic Algal Forest: Metabolomic Profiling Linked to Patterns of Genetic Diversification in the Antarctic Red Alga Plocamium sp. |
title_sort |
hidden diversity in an antarctic algal forest: metabolomic profiling linked to patterns of genetic diversification in the antarctic red alga plocamium sp. |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2021 |
url |
https://doi.org/10.3390/md19110607 |
op_coverage |
agris |
long_lat |
ENVELOPE(-63.500,-63.500,-64.600,-64.600) ENVELOPE(-63.500,-63.500,-64.600,-64.600) ENVELOPE(-114.067,-114.067,-74.833,-74.833) |
geographic |
Antarctic Anvers Anvers Island Bray The Antarctic |
geographic_facet |
Antarctic Anvers Anvers Island Bray The Antarctic |
genre |
Antarc* Antarctic Antarctica Anvers Island |
genre_facet |
Antarc* Antarctic Antarctica Anvers Island |
op_source |
Marine Drugs; Volume 19; Issue 11; Pages: 607 |
op_relation |
https://dx.doi.org/10.3390/md19110607 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/md19110607 |
container_title |
Marine Drugs |
container_volume |
19 |
container_issue |
11 |
container_start_page |
607 |
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1774717919909904384 |