Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion
The increment of non-communicable chronic diseases is a constant concern worldwide, with type-2 diabetes mellitus being one of the most common illnesses. A mechanism to avoid diabetes-related hyperglycemia is to reduce food digestion/absorption by using anti-enzymatic (functional) ingredients. This...
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ftmdpi:oai:mdpi.com:/1660-3397/18/7/353/ 2023-08-20T04:02:11+02:00 Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion Luz Verónica Pacheco Javier Parada José Ricardo Pérez-Correa María Salomé Mariotti-Celis Fernanda Erpel Angara Zambrano Mauricio Palacios agris 2020-07-08 application/pdf https://doi.org/10.3390/md18070353 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md18070353 https://creativecommons.org/licenses/by/4.0/ Marine Drugs; Volume 18; Issue 7; Pages: 353 seaweed polyphenols hypoglycemic effect starch digestion enzyme inhibition cochayuyo Text 2020 ftmdpi https://doi.org/10.3390/md18070353 2023-07-31T23:44:40Z The increment of non-communicable chronic diseases is a constant concern worldwide, with type-2 diabetes mellitus being one of the most common illnesses. A mechanism to avoid diabetes-related hyperglycemia is to reduce food digestion/absorption by using anti-enzymatic (functional) ingredients. This research explored the potential of six common Chilean seaweeds to obtain anti-hyperglycemic polyphenol extracts, based on their capacity to inhibit key enzymes related with starch digestion. Ethanol/water hot pressurized liquid extraction (HPLE), which is an environmentally friendly method, was studied and compared to conventional extraction with acetone. Total polyphenols (TP), antioxidant activity, cytotoxicity and inhibition capacity on α-glucosidase and α-amylase were analyzed. Results showed that the Durvillaea antarctica (cochayuyo) acetone extract had the highest TP content (6.7 ± 0.7 mg gallic acid equivalents (GAE)/g dry seaweed), while its HPLE ethanol/water extract showed the highest antioxidant activity (680.1 ± 11.6 μmol E Trolox/g dry seaweed). No extract affected cell viability significantly. Only cochayuyo produced extracts having relevant anti-enzymatic capacity on both studied enzymes, showing a much stronger inhibition to α-glucosidase (even almost 100% at 1000 µg/mL) than to α-amylase. In conclusion, from the Chilean seaweeds considered in this study, cochayuyo is the most suitable for developing functional ingredients to moderate postprandial glycemic response (starchy foods), since it showed a clear enzymatic inhibition capacity and selectivity. Text Antarc* Antarctica MDPI Open Access Publishing Marine Drugs 18 7 353 |
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MDPI Open Access Publishing |
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English |
topic |
seaweed polyphenols hypoglycemic effect starch digestion enzyme inhibition cochayuyo |
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seaweed polyphenols hypoglycemic effect starch digestion enzyme inhibition cochayuyo Luz Verónica Pacheco Javier Parada José Ricardo Pérez-Correa María Salomé Mariotti-Celis Fernanda Erpel Angara Zambrano Mauricio Palacios Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion |
topic_facet |
seaweed polyphenols hypoglycemic effect starch digestion enzyme inhibition cochayuyo |
description |
The increment of non-communicable chronic diseases is a constant concern worldwide, with type-2 diabetes mellitus being one of the most common illnesses. A mechanism to avoid diabetes-related hyperglycemia is to reduce food digestion/absorption by using anti-enzymatic (functional) ingredients. This research explored the potential of six common Chilean seaweeds to obtain anti-hyperglycemic polyphenol extracts, based on their capacity to inhibit key enzymes related with starch digestion. Ethanol/water hot pressurized liquid extraction (HPLE), which is an environmentally friendly method, was studied and compared to conventional extraction with acetone. Total polyphenols (TP), antioxidant activity, cytotoxicity and inhibition capacity on α-glucosidase and α-amylase were analyzed. Results showed that the Durvillaea antarctica (cochayuyo) acetone extract had the highest TP content (6.7 ± 0.7 mg gallic acid equivalents (GAE)/g dry seaweed), while its HPLE ethanol/water extract showed the highest antioxidant activity (680.1 ± 11.6 μmol E Trolox/g dry seaweed). No extract affected cell viability significantly. Only cochayuyo produced extracts having relevant anti-enzymatic capacity on both studied enzymes, showing a much stronger inhibition to α-glucosidase (even almost 100% at 1000 µg/mL) than to α-amylase. In conclusion, from the Chilean seaweeds considered in this study, cochayuyo is the most suitable for developing functional ingredients to moderate postprandial glycemic response (starchy foods), since it showed a clear enzymatic inhibition capacity and selectivity. |
format |
Text |
author |
Luz Verónica Pacheco Javier Parada José Ricardo Pérez-Correa María Salomé Mariotti-Celis Fernanda Erpel Angara Zambrano Mauricio Palacios |
author_facet |
Luz Verónica Pacheco Javier Parada José Ricardo Pérez-Correa María Salomé Mariotti-Celis Fernanda Erpel Angara Zambrano Mauricio Palacios |
author_sort |
Luz Verónica Pacheco |
title |
Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion |
title_short |
Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion |
title_full |
Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion |
title_fullStr |
Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion |
title_full_unstemmed |
Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion |
title_sort |
bioactive polyphenols from southern chile seaweed as inhibitors of enzymes for starch digestion |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/md18070353 |
op_coverage |
agris |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Marine Drugs; Volume 18; Issue 7; Pages: 353 |
op_relation |
https://dx.doi.org/10.3390/md18070353 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/md18070353 |
container_title |
Marine Drugs |
container_volume |
18 |
container_issue |
7 |
container_start_page |
353 |
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1774712563253116928 |