Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago, Antarctica
Polar marine ecosystems hold the potential for bioactive compound biodiscovery, based on their untapped macro- and microorganism diversity. Characterization of polar benthic marine invertebrate-associated microbiomes is limited to few studies. This study was motivated by our interest in better under...
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ftmdpi:oai:mdpi.com:/1660-3397/18/6/298/ 2023-08-20T04:01:50+02:00 Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago, Antarctica Alison E. Murray Nicole E. Avalon Lucas Bishop Karen W. Davenport Erwan Delage Armand E.K. Dichosa Damien Eveillard Mary L. Higham Sofia Kokkaliari Chien-Chi Lo Christian S. Riesenfeld Ryan M. Young Patrick S.G. Chain Bill J. Baker agris 2020-06-02 application/pdf https://doi.org/10.3390/md18060298 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md18060298 https://creativecommons.org/licenses/by/4.0/ Marine Drugs; Volume 18; Issue 6; Pages: 298 Antarctica ascidian microbiome microbial diversity palmerolide A co-occurrence Text 2020 ftmdpi https://doi.org/10.3390/md18060298 2023-07-31T23:35:13Z Polar marine ecosystems hold the potential for bioactive compound biodiscovery, based on their untapped macro- and microorganism diversity. Characterization of polar benthic marine invertebrate-associated microbiomes is limited to few studies. This study was motivated by our interest in better understanding the microbiome structure and composition of the ascidian, Synoicum adareanum, in which palmerolide A (PalA), a bioactive macrolide with specificity against melanoma, was isolated. PalA bears structural resemblance to a hybrid nonribosomal peptide-polyketide that has similarities to microbially-produced macrolides. We conducted a spatial survey to assess both PalA levels and microbiome composition in S. adareanum in a region of the Antarctic Peninsula near Anvers Island (64°46′ S, 64°03′ W). PalA was ubiquitous and abundant across a collection of 21 ascidians (3 subsamples each) sampled from seven sites across the Anvers Island Archipelago. The microbiome composition (V3–V4 16S rRNA gene sequence variants) of these 63 samples revealed a core suite of 21 bacterial amplicon sequence variants (ASVs)—20 of which were distinct from regional bacterioplankton. ASV co-occurrence analysis across all 63 samples yielded subgroups of taxa that may be interacting biologically (interacting subsystems) and, although the levels of PalA detected were not found to correlate with specific sequence variants, the core members appeared to occur in a preferred optimum and tolerance range of PalA levels. These results, together with an analysis of the biosynthetic potential of related microbiome taxa, describe a conserved, high-latitude core microbiome with unique composition and substantial promise for natural product biosynthesis that likely influences the ecology of the holobiont. Text Antarc* Antarctic Antarctic Peninsula Antarctica Anvers Island MDPI Open Access Publishing Antarctic Antarctic Peninsula Anvers ENVELOPE(-63.500,-63.500,-64.600,-64.600) Anvers Island ENVELOPE(-63.500,-63.500,-64.600,-64.600) Pala ENVELOPE(40.637,40.637,64.896,64.896) The Antarctic Marine Drugs 18 6 298 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Antarctica ascidian microbiome microbial diversity palmerolide A co-occurrence |
spellingShingle |
Antarctica ascidian microbiome microbial diversity palmerolide A co-occurrence Alison E. Murray Nicole E. Avalon Lucas Bishop Karen W. Davenport Erwan Delage Armand E.K. Dichosa Damien Eveillard Mary L. Higham Sofia Kokkaliari Chien-Chi Lo Christian S. Riesenfeld Ryan M. Young Patrick S.G. Chain Bill J. Baker Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago, Antarctica |
topic_facet |
Antarctica ascidian microbiome microbial diversity palmerolide A co-occurrence |
description |
Polar marine ecosystems hold the potential for bioactive compound biodiscovery, based on their untapped macro- and microorganism diversity. Characterization of polar benthic marine invertebrate-associated microbiomes is limited to few studies. This study was motivated by our interest in better understanding the microbiome structure and composition of the ascidian, Synoicum adareanum, in which palmerolide A (PalA), a bioactive macrolide with specificity against melanoma, was isolated. PalA bears structural resemblance to a hybrid nonribosomal peptide-polyketide that has similarities to microbially-produced macrolides. We conducted a spatial survey to assess both PalA levels and microbiome composition in S. adareanum in a region of the Antarctic Peninsula near Anvers Island (64°46′ S, 64°03′ W). PalA was ubiquitous and abundant across a collection of 21 ascidians (3 subsamples each) sampled from seven sites across the Anvers Island Archipelago. The microbiome composition (V3–V4 16S rRNA gene sequence variants) of these 63 samples revealed a core suite of 21 bacterial amplicon sequence variants (ASVs)—20 of which were distinct from regional bacterioplankton. ASV co-occurrence analysis across all 63 samples yielded subgroups of taxa that may be interacting biologically (interacting subsystems) and, although the levels of PalA detected were not found to correlate with specific sequence variants, the core members appeared to occur in a preferred optimum and tolerance range of PalA levels. These results, together with an analysis of the biosynthetic potential of related microbiome taxa, describe a conserved, high-latitude core microbiome with unique composition and substantial promise for natural product biosynthesis that likely influences the ecology of the holobiont. |
format |
Text |
author |
Alison E. Murray Nicole E. Avalon Lucas Bishop Karen W. Davenport Erwan Delage Armand E.K. Dichosa Damien Eveillard Mary L. Higham Sofia Kokkaliari Chien-Chi Lo Christian S. Riesenfeld Ryan M. Young Patrick S.G. Chain Bill J. Baker |
author_facet |
Alison E. Murray Nicole E. Avalon Lucas Bishop Karen W. Davenport Erwan Delage Armand E.K. Dichosa Damien Eveillard Mary L. Higham Sofia Kokkaliari Chien-Chi Lo Christian S. Riesenfeld Ryan M. Young Patrick S.G. Chain Bill J. Baker |
author_sort |
Alison E. Murray |
title |
Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago, Antarctica |
title_short |
Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago, Antarctica |
title_full |
Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago, Antarctica |
title_fullStr |
Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago, Antarctica |
title_full_unstemmed |
Uncovering the Core Microbiome and Distribution of Palmerolide in Synoicum adareanum Across the Anvers Island Archipelago, Antarctica |
title_sort |
uncovering the core microbiome and distribution of palmerolide in synoicum adareanum across the anvers island archipelago, antarctica |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/md18060298 |
op_coverage |
agris |
long_lat |
ENVELOPE(-63.500,-63.500,-64.600,-64.600) ENVELOPE(-63.500,-63.500,-64.600,-64.600) ENVELOPE(40.637,40.637,64.896,64.896) |
geographic |
Antarctic Antarctic Peninsula Anvers Anvers Island Pala The Antarctic |
geographic_facet |
Antarctic Antarctic Peninsula Anvers Anvers Island Pala The Antarctic |
genre |
Antarc* Antarctic Antarctic Peninsula Antarctica Anvers Island |
genre_facet |
Antarc* Antarctic Antarctic Peninsula Antarctica Anvers Island |
op_source |
Marine Drugs; Volume 18; Issue 6; Pages: 298 |
op_relation |
https://dx.doi.org/10.3390/md18060298 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/md18060298 |
container_title |
Marine Drugs |
container_volume |
18 |
container_issue |
6 |
container_start_page |
298 |
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1774725042605654016 |