A Novel High-Throughput Screening Platform Identifies Itaconate Derivatives from Marine Penicillium antarcticum as Inhibitors of Mesenchymal Stem Cell Differentiation
Worldwide diffused diseases such as osteoarthritis, atherosclerosis or chronic kidney disease are associated with a tissue calcification process which may involve unexpected local stem cell differentiation. Current pharmacological treatments for such musculoskeletal conditions are weakly effective,...
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ftmdpi:oai:mdpi.com:/1660-3397/18/4/192/ 2023-08-20T04:01:44+02:00 A Novel High-Throughput Screening Platform Identifies Itaconate Derivatives from Marine Penicillium antarcticum as Inhibitors of Mesenchymal Stem Cell Differentiation Pietro Marchese Nipun Mahajan Enda O’Connell Howard Fearnhead Maria Tuohy Janusz Krawczyk Olivier P. Thomas Frank Barry Mary J. Murphy agris 2020-04-05 application/pdf https://doi.org/10.3390/md18040192 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md18040192 https://creativecommons.org/licenses/by/4.0/ Marine Drugs; Volume 18; Issue 4; Pages: 192 marine fungi high throughput screening stem cell regenerative medicine drug discovery Penicillium antarcticum Text 2020 ftmdpi https://doi.org/10.3390/md18040192 2023-07-31T23:20:07Z Worldwide diffused diseases such as osteoarthritis, atherosclerosis or chronic kidney disease are associated with a tissue calcification process which may involve unexpected local stem cell differentiation. Current pharmacological treatments for such musculoskeletal conditions are weakly effective, sometimes extremely expensive and often absent. The potential to develop new therapies is represented by the discovery of small molecules modulating resident progenitor cell differentiation to prevent aberrant tissue calcification. The marine environment is a rich reserve of compounds with pharmaceutical potential and many novel molecules are isolated from macro and microorganisms annually. The potential of small molecules synthetized by marine filamentous fungi to influence the osteogenic and chondrogenic differentiation of human mesenchymal stem/stromal cells (hMSCs) was investigated using a novel, high-throughput automated screening platform. Metabolites synthetized by the marine-derived fungus Penicillium antarcticum were evaluated on the platform. Itaconic acid derivatives were identified as inhibitors of calcium elaboration into the matrix of osteogenically differentiated hMSCs and also inhibited hMSC chondrogenic differentiation, highlighting their capacity to impair ectopic calcification. Bioactive small molecule discovery is critical to address ectopic tissue calcification and the use of biologically relevant assays to identify naturally occurring metabolites from marine sources represents a strategy that can contribute to this effort. Text Antarc* MDPI Open Access Publishing Marine Drugs 18 4 192 |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
marine fungi high throughput screening stem cell regenerative medicine drug discovery Penicillium antarcticum |
spellingShingle |
marine fungi high throughput screening stem cell regenerative medicine drug discovery Penicillium antarcticum Pietro Marchese Nipun Mahajan Enda O’Connell Howard Fearnhead Maria Tuohy Janusz Krawczyk Olivier P. Thomas Frank Barry Mary J. Murphy A Novel High-Throughput Screening Platform Identifies Itaconate Derivatives from Marine Penicillium antarcticum as Inhibitors of Mesenchymal Stem Cell Differentiation |
topic_facet |
marine fungi high throughput screening stem cell regenerative medicine drug discovery Penicillium antarcticum |
description |
Worldwide diffused diseases such as osteoarthritis, atherosclerosis or chronic kidney disease are associated with a tissue calcification process which may involve unexpected local stem cell differentiation. Current pharmacological treatments for such musculoskeletal conditions are weakly effective, sometimes extremely expensive and often absent. The potential to develop new therapies is represented by the discovery of small molecules modulating resident progenitor cell differentiation to prevent aberrant tissue calcification. The marine environment is a rich reserve of compounds with pharmaceutical potential and many novel molecules are isolated from macro and microorganisms annually. The potential of small molecules synthetized by marine filamentous fungi to influence the osteogenic and chondrogenic differentiation of human mesenchymal stem/stromal cells (hMSCs) was investigated using a novel, high-throughput automated screening platform. Metabolites synthetized by the marine-derived fungus Penicillium antarcticum were evaluated on the platform. Itaconic acid derivatives were identified as inhibitors of calcium elaboration into the matrix of osteogenically differentiated hMSCs and also inhibited hMSC chondrogenic differentiation, highlighting their capacity to impair ectopic calcification. Bioactive small molecule discovery is critical to address ectopic tissue calcification and the use of biologically relevant assays to identify naturally occurring metabolites from marine sources represents a strategy that can contribute to this effort. |
format |
Text |
author |
Pietro Marchese Nipun Mahajan Enda O’Connell Howard Fearnhead Maria Tuohy Janusz Krawczyk Olivier P. Thomas Frank Barry Mary J. Murphy |
author_facet |
Pietro Marchese Nipun Mahajan Enda O’Connell Howard Fearnhead Maria Tuohy Janusz Krawczyk Olivier P. Thomas Frank Barry Mary J. Murphy |
author_sort |
Pietro Marchese |
title |
A Novel High-Throughput Screening Platform Identifies Itaconate Derivatives from Marine Penicillium antarcticum as Inhibitors of Mesenchymal Stem Cell Differentiation |
title_short |
A Novel High-Throughput Screening Platform Identifies Itaconate Derivatives from Marine Penicillium antarcticum as Inhibitors of Mesenchymal Stem Cell Differentiation |
title_full |
A Novel High-Throughput Screening Platform Identifies Itaconate Derivatives from Marine Penicillium antarcticum as Inhibitors of Mesenchymal Stem Cell Differentiation |
title_fullStr |
A Novel High-Throughput Screening Platform Identifies Itaconate Derivatives from Marine Penicillium antarcticum as Inhibitors of Mesenchymal Stem Cell Differentiation |
title_full_unstemmed |
A Novel High-Throughput Screening Platform Identifies Itaconate Derivatives from Marine Penicillium antarcticum as Inhibitors of Mesenchymal Stem Cell Differentiation |
title_sort |
novel high-throughput screening platform identifies itaconate derivatives from marine penicillium antarcticum as inhibitors of mesenchymal stem cell differentiation |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/md18040192 |
op_coverage |
agris |
genre |
Antarc* |
genre_facet |
Antarc* |
op_source |
Marine Drugs; Volume 18; Issue 4; Pages: 192 |
op_relation |
https://dx.doi.org/10.3390/md18040192 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/md18040192 |
container_title |
Marine Drugs |
container_volume |
18 |
container_issue |
4 |
container_start_page |
192 |
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1774724959321456640 |