Temperature Differentially Affects Gene Expression in Antarctic Thraustochytrid Oblongichytrium sp. RT2316-13
Oblongichytrium RT2316-13 synthesizes lipids rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The content of these fatty acids in the total lipids depended on growth temperature. Sequencing technology was used in this work to examine the thraustochytrid’s response to a decrease in...
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ftmdpi:oai:mdpi.com:/1660-3397/18/11/563/ 2023-08-20T04:02:33+02:00 Temperature Differentially Affects Gene Expression in Antarctic Thraustochytrid Oblongichytrium sp. RT2316-13 Paris Paredes Giovanni Larama Liset Flores Allison Leyton Carmen Gloria Ili Juan A. Asenjo Yusuf Chisti Carolina Shene agris 2020-11-18 application/pdf https://doi.org/10.3390/md18110563 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md18110563 https://creativecommons.org/licenses/by/4.0/ Marine Drugs; Volume 18; Issue 11; Pages: 563 gene expression analysis lipid metabolism Oblongichytrium sp. polyunsaturated fatty acid synthesis thraustochytrids Text 2020 ftmdpi https://doi.org/10.3390/md18110563 2023-08-01T00:29:00Z Oblongichytrium RT2316-13 synthesizes lipids rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The content of these fatty acids in the total lipids depended on growth temperature. Sequencing technology was used in this work to examine the thraustochytrid’s response to a decrease in growth temperature from 15 °C to 5 °C. Around 4% (2944) of the genes were differentially expressed (DE) and only a few of the DE genes (533 upregulated; 206 downregulated) had significant matches to those in the SwissProt database. Most of the annotated DE genes were related to cell membrane composition (fatty acids, sterols, phosphatidylinositol), the membrane enzymes linked to cell energetics, and membrane structure (cytoskeletal proteins and enzymes). In RT2316-13, the synthesis of long-chain polyunsaturated fatty acids occurred through ω3- and ω6-pathways. Enzymes of the alternative pathways (Δ8-desaturase and Δ9-elongase) were also expressed. The upregulation of the genes coding for a Δ5-desaturase and a Δ5-elongase involved in the synthesis of EPA and DHA, explained the enrichment of total lipid with these two long-chain fatty acids at the low temperature. This molecular response has the potential to be used for producing microbial lipids with a fatty acids profile similar to that of fish oils. Text Antarc* Antarctic MDPI Open Access Publishing Antarctic Marine Drugs 18 11 563 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
gene expression analysis lipid metabolism Oblongichytrium sp. polyunsaturated fatty acid synthesis thraustochytrids |
spellingShingle |
gene expression analysis lipid metabolism Oblongichytrium sp. polyunsaturated fatty acid synthesis thraustochytrids Paris Paredes Giovanni Larama Liset Flores Allison Leyton Carmen Gloria Ili Juan A. Asenjo Yusuf Chisti Carolina Shene Temperature Differentially Affects Gene Expression in Antarctic Thraustochytrid Oblongichytrium sp. RT2316-13 |
topic_facet |
gene expression analysis lipid metabolism Oblongichytrium sp. polyunsaturated fatty acid synthesis thraustochytrids |
description |
Oblongichytrium RT2316-13 synthesizes lipids rich in eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). The content of these fatty acids in the total lipids depended on growth temperature. Sequencing technology was used in this work to examine the thraustochytrid’s response to a decrease in growth temperature from 15 °C to 5 °C. Around 4% (2944) of the genes were differentially expressed (DE) and only a few of the DE genes (533 upregulated; 206 downregulated) had significant matches to those in the SwissProt database. Most of the annotated DE genes were related to cell membrane composition (fatty acids, sterols, phosphatidylinositol), the membrane enzymes linked to cell energetics, and membrane structure (cytoskeletal proteins and enzymes). In RT2316-13, the synthesis of long-chain polyunsaturated fatty acids occurred through ω3- and ω6-pathways. Enzymes of the alternative pathways (Δ8-desaturase and Δ9-elongase) were also expressed. The upregulation of the genes coding for a Δ5-desaturase and a Δ5-elongase involved in the synthesis of EPA and DHA, explained the enrichment of total lipid with these two long-chain fatty acids at the low temperature. This molecular response has the potential to be used for producing microbial lipids with a fatty acids profile similar to that of fish oils. |
format |
Text |
author |
Paris Paredes Giovanni Larama Liset Flores Allison Leyton Carmen Gloria Ili Juan A. Asenjo Yusuf Chisti Carolina Shene |
author_facet |
Paris Paredes Giovanni Larama Liset Flores Allison Leyton Carmen Gloria Ili Juan A. Asenjo Yusuf Chisti Carolina Shene |
author_sort |
Paris Paredes |
title |
Temperature Differentially Affects Gene Expression in Antarctic Thraustochytrid Oblongichytrium sp. RT2316-13 |
title_short |
Temperature Differentially Affects Gene Expression in Antarctic Thraustochytrid Oblongichytrium sp. RT2316-13 |
title_full |
Temperature Differentially Affects Gene Expression in Antarctic Thraustochytrid Oblongichytrium sp. RT2316-13 |
title_fullStr |
Temperature Differentially Affects Gene Expression in Antarctic Thraustochytrid Oblongichytrium sp. RT2316-13 |
title_full_unstemmed |
Temperature Differentially Affects Gene Expression in Antarctic Thraustochytrid Oblongichytrium sp. RT2316-13 |
title_sort |
temperature differentially affects gene expression in antarctic thraustochytrid oblongichytrium sp. rt2316-13 |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/md18110563 |
op_coverage |
agris |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Marine Drugs; Volume 18; Issue 11; Pages: 563 |
op_relation |
https://dx.doi.org/10.3390/md18110563 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/md18110563 |
container_title |
Marine Drugs |
container_volume |
18 |
container_issue |
11 |
container_start_page |
563 |
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1774713063487832064 |