Applying a Chemogeographic Strategy for Natural Product Discovery from the Marine Cyanobacterium Moorena bouillonii
The tropical marine cyanobacterium Moorena bouillonii occupies a large geographic range across the Indian and Western Tropical Pacific Oceans and is a prolific producer of structurally unique and biologically active natural products. An ensemble of computational approaches, including the creation of...
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ftmdpi:oai:mdpi.com:/1660-3397/18/10/515/ 2023-08-20T04:09:05+02:00 Applying a Chemogeographic Strategy for Natural Product Discovery from the Marine Cyanobacterium Moorena bouillonii Christopher A. Leber C. Benjamin Naman Lena Keller Jehad Almaliti Eduardo J. E. Caro-Diaz Evgenia Glukhov Valsamma Joseph T. P. Sajeevan Andres Joshua Reyes Jason S. Biggs Te Li Ye Yuan Shan He Xiaojun Yan William H. Gerwick agris 2020-10-14 application/pdf https://doi.org/10.3390/md18100515 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md18100515 https://creativecommons.org/licenses/by/4.0/ Marine Drugs; Volume 18; Issue 10; Pages: 515 Moorena bouillonii marine natural products chemogeography metabolomics Text 2020 ftmdpi https://doi.org/10.3390/md18100515 2023-08-01T00:16:18Z The tropical marine cyanobacterium Moorena bouillonii occupies a large geographic range across the Indian and Western Tropical Pacific Oceans and is a prolific producer of structurally unique and biologically active natural products. An ensemble of computational approaches, including the creation of the ORCA (Objective Relational Comparative Analysis) pipeline for flexible MS1 feature detection and multivariate analyses, were used to analyze various M. bouillonii samples. The observed chemogeographic patterns suggested the production of regionally specific natural products by M. bouillonii. Analyzing the drivers of these chemogeographic patterns allowed for the identification, targeted isolation, and structure elucidation of a regionally specific natural product, doscadenamide A (1). Analyses of MS2 fragmentation patterns further revealed this natural product to be part of an extensive family of herein annotated, proposed natural structural analogs (doscadenamides B–J, 2–10); the ensemble of structures reflect a combinatorial biosynthesis using nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) components. Compound 1 displayed synergistic in vitro cancer cell cytotoxicity when administered with lipopolysaccharide (LPS). These discoveries illustrate the utility in leveraging chemogeographic patterns for prioritizing natural product discovery efforts. Text Orca MDPI Open Access Publishing Indian Pacific Marine Drugs 18 10 515 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Moorena bouillonii marine natural products chemogeography metabolomics |
spellingShingle |
Moorena bouillonii marine natural products chemogeography metabolomics Christopher A. Leber C. Benjamin Naman Lena Keller Jehad Almaliti Eduardo J. E. Caro-Diaz Evgenia Glukhov Valsamma Joseph T. P. Sajeevan Andres Joshua Reyes Jason S. Biggs Te Li Ye Yuan Shan He Xiaojun Yan William H. Gerwick Applying a Chemogeographic Strategy for Natural Product Discovery from the Marine Cyanobacterium Moorena bouillonii |
topic_facet |
Moorena bouillonii marine natural products chemogeography metabolomics |
description |
The tropical marine cyanobacterium Moorena bouillonii occupies a large geographic range across the Indian and Western Tropical Pacific Oceans and is a prolific producer of structurally unique and biologically active natural products. An ensemble of computational approaches, including the creation of the ORCA (Objective Relational Comparative Analysis) pipeline for flexible MS1 feature detection and multivariate analyses, were used to analyze various M. bouillonii samples. The observed chemogeographic patterns suggested the production of regionally specific natural products by M. bouillonii. Analyzing the drivers of these chemogeographic patterns allowed for the identification, targeted isolation, and structure elucidation of a regionally specific natural product, doscadenamide A (1). Analyses of MS2 fragmentation patterns further revealed this natural product to be part of an extensive family of herein annotated, proposed natural structural analogs (doscadenamides B–J, 2–10); the ensemble of structures reflect a combinatorial biosynthesis using nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) components. Compound 1 displayed synergistic in vitro cancer cell cytotoxicity when administered with lipopolysaccharide (LPS). These discoveries illustrate the utility in leveraging chemogeographic patterns for prioritizing natural product discovery efforts. |
format |
Text |
author |
Christopher A. Leber C. Benjamin Naman Lena Keller Jehad Almaliti Eduardo J. E. Caro-Diaz Evgenia Glukhov Valsamma Joseph T. P. Sajeevan Andres Joshua Reyes Jason S. Biggs Te Li Ye Yuan Shan He Xiaojun Yan William H. Gerwick |
author_facet |
Christopher A. Leber C. Benjamin Naman Lena Keller Jehad Almaliti Eduardo J. E. Caro-Diaz Evgenia Glukhov Valsamma Joseph T. P. Sajeevan Andres Joshua Reyes Jason S. Biggs Te Li Ye Yuan Shan He Xiaojun Yan William H. Gerwick |
author_sort |
Christopher A. Leber |
title |
Applying a Chemogeographic Strategy for Natural Product Discovery from the Marine Cyanobacterium Moorena bouillonii |
title_short |
Applying a Chemogeographic Strategy for Natural Product Discovery from the Marine Cyanobacterium Moorena bouillonii |
title_full |
Applying a Chemogeographic Strategy for Natural Product Discovery from the Marine Cyanobacterium Moorena bouillonii |
title_fullStr |
Applying a Chemogeographic Strategy for Natural Product Discovery from the Marine Cyanobacterium Moorena bouillonii |
title_full_unstemmed |
Applying a Chemogeographic Strategy for Natural Product Discovery from the Marine Cyanobacterium Moorena bouillonii |
title_sort |
applying a chemogeographic strategy for natural product discovery from the marine cyanobacterium moorena bouillonii |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/md18100515 |
op_coverage |
agris |
geographic |
Indian Pacific |
geographic_facet |
Indian Pacific |
genre |
Orca |
genre_facet |
Orca |
op_source |
Marine Drugs; Volume 18; Issue 10; Pages: 515 |
op_relation |
https://dx.doi.org/10.3390/md18100515 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/md18100515 |
container_title |
Marine Drugs |
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18 |
container_issue |
10 |
container_start_page |
515 |
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