Synthesis of Bioactive Silver Nanoparticles by a Pseudomonas Strain Associated with the Antarctic Psychrophilic Protozoon Euplotes focardii
The synthesis of silver nanoparticles (AgNPs) by microorganisms recently gained a greater interest due to its potential to produce them in various sizes and morphologies. In this study, for AgNP biosynthesis, we used a new Pseudomonas strain isolated from a consortium associated with the Antarctic m...
Published in: | Marine Drugs |
---|---|
Main Authors: | , , , , , , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2020
|
Subjects: | |
Online Access: | https://doi.org/10.3390/md18010038 |
id |
ftmdpi:oai:mdpi.com:/1660-3397/18/1/38/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/1660-3397/18/1/38/ 2023-08-20T04:00:45+02:00 Synthesis of Bioactive Silver Nanoparticles by a Pseudomonas Strain Associated with the Antarctic Psychrophilic Protozoon Euplotes focardii Maria Sindhura John Joseph Amruthraj Nagoth Kesava Priyan Ramasamy Alessio Mancini Gabriele Giuli Antonino Natalello Patrizia Ballarini Cristina Miceli Sandra Pucciarelli agris 2020-01-03 application/pdf https://doi.org/10.3390/md18010038 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md18010038 https://creativecommons.org/licenses/by/4.0/ Marine Drugs; Volume 18; Issue 1; Pages: 38 green synthesis biomaterials silver nitrate antibiotics nanotechnology Text 2020 ftmdpi https://doi.org/10.3390/md18010038 2023-07-31T22:57:50Z The synthesis of silver nanoparticles (AgNPs) by microorganisms recently gained a greater interest due to its potential to produce them in various sizes and morphologies. In this study, for AgNP biosynthesis, we used a new Pseudomonas strain isolated from a consortium associated with the Antarctic marine ciliate Euplotes focardii. After incubation of Pseudomonas cultures with 1 mM of AgNO3 at 22 °C, we obtained AgNPs within 24 h. Scanning electron (SEM) and transmission electron microscopy (TEM) revealed spherical polydispersed AgNPs in the size range of 20–70 nm. The average size was approximately 50 nm. Energy dispersive X-ray spectroscopy (EDS) showed the presence of a high intensity absorption peak at 3 keV, a distinctive property of nanocrystalline silver products. Fourier transform infrared (FTIR) spectroscopy found the presence of a high amount of AgNP-stabilizing proteins and other secondary metabolites. X-ray diffraction (XRD) revealed a face-centred cubic (fcc) diffraction spectrum with a crystalline nature. A comparative study between the chemically synthesized and Pseudomonas AgNPs revealed a higher antibacterial activity of the latter against common nosocomial pathogen microorganisms, including Escherichia coli, Staphylococcus aureus and Candida albicans. This study reports an efficient, rapid synthesis of stable AgNPs by a new Pseudomonas strain with high antimicrobial activity. Text Antarc* Antarctic MDPI Open Access Publishing Antarctic The Antarctic Marine Drugs 18 1 38 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
green synthesis biomaterials silver nitrate antibiotics nanotechnology |
spellingShingle |
green synthesis biomaterials silver nitrate antibiotics nanotechnology Maria Sindhura John Joseph Amruthraj Nagoth Kesava Priyan Ramasamy Alessio Mancini Gabriele Giuli Antonino Natalello Patrizia Ballarini Cristina Miceli Sandra Pucciarelli Synthesis of Bioactive Silver Nanoparticles by a Pseudomonas Strain Associated with the Antarctic Psychrophilic Protozoon Euplotes focardii |
topic_facet |
green synthesis biomaterials silver nitrate antibiotics nanotechnology |
description |
The synthesis of silver nanoparticles (AgNPs) by microorganisms recently gained a greater interest due to its potential to produce them in various sizes and morphologies. In this study, for AgNP biosynthesis, we used a new Pseudomonas strain isolated from a consortium associated with the Antarctic marine ciliate Euplotes focardii. After incubation of Pseudomonas cultures with 1 mM of AgNO3 at 22 °C, we obtained AgNPs within 24 h. Scanning electron (SEM) and transmission electron microscopy (TEM) revealed spherical polydispersed AgNPs in the size range of 20–70 nm. The average size was approximately 50 nm. Energy dispersive X-ray spectroscopy (EDS) showed the presence of a high intensity absorption peak at 3 keV, a distinctive property of nanocrystalline silver products. Fourier transform infrared (FTIR) spectroscopy found the presence of a high amount of AgNP-stabilizing proteins and other secondary metabolites. X-ray diffraction (XRD) revealed a face-centred cubic (fcc) diffraction spectrum with a crystalline nature. A comparative study between the chemically synthesized and Pseudomonas AgNPs revealed a higher antibacterial activity of the latter against common nosocomial pathogen microorganisms, including Escherichia coli, Staphylococcus aureus and Candida albicans. This study reports an efficient, rapid synthesis of stable AgNPs by a new Pseudomonas strain with high antimicrobial activity. |
format |
Text |
author |
Maria Sindhura John Joseph Amruthraj Nagoth Kesava Priyan Ramasamy Alessio Mancini Gabriele Giuli Antonino Natalello Patrizia Ballarini Cristina Miceli Sandra Pucciarelli |
author_facet |
Maria Sindhura John Joseph Amruthraj Nagoth Kesava Priyan Ramasamy Alessio Mancini Gabriele Giuli Antonino Natalello Patrizia Ballarini Cristina Miceli Sandra Pucciarelli |
author_sort |
Maria Sindhura John |
title |
Synthesis of Bioactive Silver Nanoparticles by a Pseudomonas Strain Associated with the Antarctic Psychrophilic Protozoon Euplotes focardii |
title_short |
Synthesis of Bioactive Silver Nanoparticles by a Pseudomonas Strain Associated with the Antarctic Psychrophilic Protozoon Euplotes focardii |
title_full |
Synthesis of Bioactive Silver Nanoparticles by a Pseudomonas Strain Associated with the Antarctic Psychrophilic Protozoon Euplotes focardii |
title_fullStr |
Synthesis of Bioactive Silver Nanoparticles by a Pseudomonas Strain Associated with the Antarctic Psychrophilic Protozoon Euplotes focardii |
title_full_unstemmed |
Synthesis of Bioactive Silver Nanoparticles by a Pseudomonas Strain Associated with the Antarctic Psychrophilic Protozoon Euplotes focardii |
title_sort |
synthesis of bioactive silver nanoparticles by a pseudomonas strain associated with the antarctic psychrophilic protozoon euplotes focardii |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2020 |
url |
https://doi.org/10.3390/md18010038 |
op_coverage |
agris |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Marine Drugs; Volume 18; Issue 1; Pages: 38 |
op_relation |
https://dx.doi.org/10.3390/md18010038 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/md18010038 |
container_title |
Marine Drugs |
container_volume |
18 |
container_issue |
1 |
container_start_page |
38 |
_version_ |
1774720107389386752 |