Structural Elucidation of Irish Organic Farmed Salmon (Salmo salar) Polar Lipids with Antithrombotic Activities
While several marine polar lipids (PL) have exhibited cardioprotective properties through their effects on the platelet-activating factor (PAF) pathways, salmon PL have not been tested so far. In this study, the antithrombotic activities of salmon PL were assessed in human platelets and the structur...
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ftmdpi:oai:mdpi.com:/1660-3397/16/6/176/ 2023-08-20T04:09:31+02:00 Structural Elucidation of Irish Organic Farmed Salmon (Salmo salar) Polar Lipids with Antithrombotic Activities Alexandros Tsoupras Ronan Lordan Martina Demuru Katie Shiels Sushanta Saha Constantina Nasopoulou Ioannis Zabetakis agris 2018-05-23 application/pdf https://doi.org/10.3390/md16060176 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md16060176 https://creativecommons.org/licenses/by/4.0/ Marine Drugs; Volume 16; Issue 6; Pages: 176 salmon polar lipids platelet aggregation platelet-activating factor (PAF) thrombin antithrombotic LC-MS GC-MS phosphatidylcholine phosphatidylethanolamine polyunsaturated fatty acids (PUFA) docosahexaenoic acid (DHA) Text 2018 ftmdpi https://doi.org/10.3390/md16060176 2023-07-31T21:32:28Z While several marine polar lipids (PL) have exhibited cardioprotective properties through their effects on the platelet-activating factor (PAF) pathways, salmon PL have not been tested so far. In this study, the antithrombotic activities of salmon PL were assessed in human platelets and the structural characterisation of bioactive salmon PL was performed by GC-MS and LC-MS analyses. PL from fillets of Irish organic farmed salmon (Salmo salar) were extracted and separated into several lipid subclasses by thin-layer chromatography (TLC), while their fatty acid profile was fully characterised by GC-MS. Salmon total lipids (TL), total neutral lipids (TNL), total polar lipids (TPL), and each PL subclass obtained by TLC were further assessed for their in vitro effects towards PAF-induced and thrombin-induced platelet aggregation in human platelets. Salmon PL exhibited antithrombotic effects on human platelet aggregation, mostly through their strong inhibitory effects against the PAF pathway with IC50 values comparable to other marine PL, but with lower effects towards the thrombin pathway. PL fractions corresponding to phosphatidylcholine and phosphatidylethanolamine derivatives exhibited the most potent anti-PAF effects, while LC-MS analysis putatively elucidated their structure/function relationship. Several diacyl-PC/PE and alkyl-acyl-PC/PE species containing mostly docosahexaenoic acid at their sn-2 glycerol-backbone may be responsible for the bioactivity. The data presented suggests that salmon contains PL with strong antithrombotic bioactivities. Text Salmo salar MDPI Open Access Publishing Marine Drugs 16 6 176 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
salmon polar lipids platelet aggregation platelet-activating factor (PAF) thrombin antithrombotic LC-MS GC-MS phosphatidylcholine phosphatidylethanolamine polyunsaturated fatty acids (PUFA) docosahexaenoic acid (DHA) |
spellingShingle |
salmon polar lipids platelet aggregation platelet-activating factor (PAF) thrombin antithrombotic LC-MS GC-MS phosphatidylcholine phosphatidylethanolamine polyunsaturated fatty acids (PUFA) docosahexaenoic acid (DHA) Alexandros Tsoupras Ronan Lordan Martina Demuru Katie Shiels Sushanta Saha Constantina Nasopoulou Ioannis Zabetakis Structural Elucidation of Irish Organic Farmed Salmon (Salmo salar) Polar Lipids with Antithrombotic Activities |
topic_facet |
salmon polar lipids platelet aggregation platelet-activating factor (PAF) thrombin antithrombotic LC-MS GC-MS phosphatidylcholine phosphatidylethanolamine polyunsaturated fatty acids (PUFA) docosahexaenoic acid (DHA) |
description |
While several marine polar lipids (PL) have exhibited cardioprotective properties through their effects on the platelet-activating factor (PAF) pathways, salmon PL have not been tested so far. In this study, the antithrombotic activities of salmon PL were assessed in human platelets and the structural characterisation of bioactive salmon PL was performed by GC-MS and LC-MS analyses. PL from fillets of Irish organic farmed salmon (Salmo salar) were extracted and separated into several lipid subclasses by thin-layer chromatography (TLC), while their fatty acid profile was fully characterised by GC-MS. Salmon total lipids (TL), total neutral lipids (TNL), total polar lipids (TPL), and each PL subclass obtained by TLC were further assessed for their in vitro effects towards PAF-induced and thrombin-induced platelet aggregation in human platelets. Salmon PL exhibited antithrombotic effects on human platelet aggregation, mostly through their strong inhibitory effects against the PAF pathway with IC50 values comparable to other marine PL, but with lower effects towards the thrombin pathway. PL fractions corresponding to phosphatidylcholine and phosphatidylethanolamine derivatives exhibited the most potent anti-PAF effects, while LC-MS analysis putatively elucidated their structure/function relationship. Several diacyl-PC/PE and alkyl-acyl-PC/PE species containing mostly docosahexaenoic acid at their sn-2 glycerol-backbone may be responsible for the bioactivity. The data presented suggests that salmon contains PL with strong antithrombotic bioactivities. |
format |
Text |
author |
Alexandros Tsoupras Ronan Lordan Martina Demuru Katie Shiels Sushanta Saha Constantina Nasopoulou Ioannis Zabetakis |
author_facet |
Alexandros Tsoupras Ronan Lordan Martina Demuru Katie Shiels Sushanta Saha Constantina Nasopoulou Ioannis Zabetakis |
author_sort |
Alexandros Tsoupras |
title |
Structural Elucidation of Irish Organic Farmed Salmon (Salmo salar) Polar Lipids with Antithrombotic Activities |
title_short |
Structural Elucidation of Irish Organic Farmed Salmon (Salmo salar) Polar Lipids with Antithrombotic Activities |
title_full |
Structural Elucidation of Irish Organic Farmed Salmon (Salmo salar) Polar Lipids with Antithrombotic Activities |
title_fullStr |
Structural Elucidation of Irish Organic Farmed Salmon (Salmo salar) Polar Lipids with Antithrombotic Activities |
title_full_unstemmed |
Structural Elucidation of Irish Organic Farmed Salmon (Salmo salar) Polar Lipids with Antithrombotic Activities |
title_sort |
structural elucidation of irish organic farmed salmon (salmo salar) polar lipids with antithrombotic activities |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2018 |
url |
https://doi.org/10.3390/md16060176 |
op_coverage |
agris |
genre |
Salmo salar |
genre_facet |
Salmo salar |
op_source |
Marine Drugs; Volume 16; Issue 6; Pages: 176 |
op_relation |
https://dx.doi.org/10.3390/md16060176 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/md16060176 |
container_title |
Marine Drugs |
container_volume |
16 |
container_issue |
6 |
container_start_page |
176 |
_version_ |
1774722520170102784 |