Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group
The marine environment is a rich source of biodiversity, including microorganisms that have proven to be prolific producers of bioactive secondary metabolites. Arctic seas are less explored than warmer, more accessible areas, providing a promising starting point to search for novel bioactive compoun...
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Online Access: | https://doi.org/10.3390/md16050163 |
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ftmdpi:oai:mdpi.com:/1660-3397/16/5/163/ 2023-08-20T04:03:52+02:00 Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group Venke Kristoffersen Teppo Rämä Johan Isaksson Jeanette Andersen William Gerwick Espen Hansen agris 2018-05-14 application/pdf https://doi.org/10.3390/md16050163 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md16050163 https://creativecommons.org/licenses/by/4.0/ Marine Drugs; Volume 16; Issue 5; Pages: 163 arctic bacteria bioactive OSMAC (one strain many compounds) molecular networking rhamnolipids Text 2018 ftmdpi https://doi.org/10.3390/md16050163 2023-07-31T21:31:15Z The marine environment is a rich source of biodiversity, including microorganisms that have proven to be prolific producers of bioactive secondary metabolites. Arctic seas are less explored than warmer, more accessible areas, providing a promising starting point to search for novel bioactive compounds. In the present work, an Arctic marine Pseudomonas sp. belonging to the Pseudomonas (P.) fluorescence group was cultivated in four different media in an attempt to activate biosynthetic pathways leading to the production of antibacterial and anticancer compounds. Culture extracts were pre-fractionated and screened for antibacterial and anticancer activities. One fraction from three of the four growth conditions showed inhibitory activity towards bacteria and cancer cells. The active fractions were dereplicated using molecular networking based on MS/MS fragmentation data, indicating the presence of a cluster of related rhamnolipids. Six compounds were isolated using HPLC and mass-guided fractionation, and by interpreting data from NMR and high-resolution MS/MS analysis; the structures of the compounds were determined to be five mono-rhamnolipids and the lipid moiety of one of the rhamnolipids. Molecular networking proved to be a valuable tool for dereplication of these related compounds, and for the first time, five mono-rhamnolipids from a bacterium within the P. fluorescence group were characterized, including one new mono-rhamnolipid. Text Arctic MDPI Open Access Publishing Arctic Marine Drugs 16 5 163 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
arctic bacteria bioactive OSMAC (one strain many compounds) molecular networking rhamnolipids |
spellingShingle |
arctic bacteria bioactive OSMAC (one strain many compounds) molecular networking rhamnolipids Venke Kristoffersen Teppo Rämä Johan Isaksson Jeanette Andersen William Gerwick Espen Hansen Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group |
topic_facet |
arctic bacteria bioactive OSMAC (one strain many compounds) molecular networking rhamnolipids |
description |
The marine environment is a rich source of biodiversity, including microorganisms that have proven to be prolific producers of bioactive secondary metabolites. Arctic seas are less explored than warmer, more accessible areas, providing a promising starting point to search for novel bioactive compounds. In the present work, an Arctic marine Pseudomonas sp. belonging to the Pseudomonas (P.) fluorescence group was cultivated in four different media in an attempt to activate biosynthetic pathways leading to the production of antibacterial and anticancer compounds. Culture extracts were pre-fractionated and screened for antibacterial and anticancer activities. One fraction from three of the four growth conditions showed inhibitory activity towards bacteria and cancer cells. The active fractions were dereplicated using molecular networking based on MS/MS fragmentation data, indicating the presence of a cluster of related rhamnolipids. Six compounds were isolated using HPLC and mass-guided fractionation, and by interpreting data from NMR and high-resolution MS/MS analysis; the structures of the compounds were determined to be five mono-rhamnolipids and the lipid moiety of one of the rhamnolipids. Molecular networking proved to be a valuable tool for dereplication of these related compounds, and for the first time, five mono-rhamnolipids from a bacterium within the P. fluorescence group were characterized, including one new mono-rhamnolipid. |
format |
Text |
author |
Venke Kristoffersen Teppo Rämä Johan Isaksson Jeanette Andersen William Gerwick Espen Hansen |
author_facet |
Venke Kristoffersen Teppo Rämä Johan Isaksson Jeanette Andersen William Gerwick Espen Hansen |
author_sort |
Venke Kristoffersen |
title |
Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group |
title_short |
Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group |
title_full |
Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group |
title_fullStr |
Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group |
title_full_unstemmed |
Characterization of Rhamnolipids Produced by an Arctic Marine Bacterium from the Pseudomonas fluorescence Group |
title_sort |
characterization of rhamnolipids produced by an arctic marine bacterium from the pseudomonas fluorescence group |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2018 |
url |
https://doi.org/10.3390/md16050163 |
op_coverage |
agris |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Marine Drugs; Volume 16; Issue 5; Pages: 163 |
op_relation |
https://dx.doi.org/10.3390/md16050163 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/md16050163 |
container_title |
Marine Drugs |
container_volume |
16 |
container_issue |
5 |
container_start_page |
163 |
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1774714297037881344 |