Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements
Menaquinone (MK) has an important role in human metabolism as an essential vitamin (VK2), which is mainly produced through the fermentation of microorganisms. MK8(H2) was identified to be the main menaquinone from Rhodococcus sp. B7740, a bacterium isolated from the arctic ocean. In this work, MK8(H...
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ftmdpi:oai:mdpi.com:/1660-3397/16/10/391/ 2023-08-20T04:04:12+02:00 Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements Yashu Chen Qin Mu Kai Hu Mo Chen Jifang Yang Jigang Chen Bijun Xie Zhida Sun agris 2018-10-18 application/pdf https://doi.org/10.3390/md16100391 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md16100391 https://creativecommons.org/licenses/by/4.0/ Marine Drugs; Volume 16; Issue 10; Pages: 391 isoprenoid quinone MK 8 (H 2 ) Rhodococcus sp. B7740 from Arctic ocean UPLC-HRMS NMR antioxidant and antiglycation Text 2018 ftmdpi https://doi.org/10.3390/md16100391 2023-07-31T21:47:16Z Menaquinone (MK) has an important role in human metabolism as an essential vitamin (VK2), which is mainly produced through the fermentation of microorganisms. MK8(H2) was identified to be the main menaquinone from Rhodococcus sp. B7740, a bacterium isolated from the arctic ocean. In this work, MK8(H2) (purity: 99.75%) was collected through a convenient and economic extraction process followed by high-speed countercurrent chromatography (HSCCC) purification. Additionally, high-resolution mass spectrometry (HRMS) was performed for further identification and the hydrogenation position of MK8(H2) (terminal unit) was determined using nuclear magnetic resonance (NMR) for the first time. MK8(H2) showed a superior antioxidant effect and antiglycation capacity compared with ubiquinone Q10 and MK4. High-performance liquid chromatography–mass spectrometer (HPLC-MS/MS) and molecular docking showed the fine interaction between MK8(H2) with methylglyoxal (MGO) and bull serum albumin (BSA), respectively. These properties make MK8(H2) a promising natural active ingredient with future food and medicine applications. Text Arctic Arctic Ocean MDPI Open Access Publishing Arctic Arctic Ocean Marine Drugs 16 10 391 |
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Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
isoprenoid quinone MK 8 (H 2 ) Rhodococcus sp. B7740 from Arctic ocean UPLC-HRMS NMR antioxidant and antiglycation |
spellingShingle |
isoprenoid quinone MK 8 (H 2 ) Rhodococcus sp. B7740 from Arctic ocean UPLC-HRMS NMR antioxidant and antiglycation Yashu Chen Qin Mu Kai Hu Mo Chen Jifang Yang Jigang Chen Bijun Xie Zhida Sun Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements |
topic_facet |
isoprenoid quinone MK 8 (H 2 ) Rhodococcus sp. B7740 from Arctic ocean UPLC-HRMS NMR antioxidant and antiglycation |
description |
Menaquinone (MK) has an important role in human metabolism as an essential vitamin (VK2), which is mainly produced through the fermentation of microorganisms. MK8(H2) was identified to be the main menaquinone from Rhodococcus sp. B7740, a bacterium isolated from the arctic ocean. In this work, MK8(H2) (purity: 99.75%) was collected through a convenient and economic extraction process followed by high-speed countercurrent chromatography (HSCCC) purification. Additionally, high-resolution mass spectrometry (HRMS) was performed for further identification and the hydrogenation position of MK8(H2) (terminal unit) was determined using nuclear magnetic resonance (NMR) for the first time. MK8(H2) showed a superior antioxidant effect and antiglycation capacity compared with ubiquinone Q10 and MK4. High-performance liquid chromatography–mass spectrometer (HPLC-MS/MS) and molecular docking showed the fine interaction between MK8(H2) with methylglyoxal (MGO) and bull serum albumin (BSA), respectively. These properties make MK8(H2) a promising natural active ingredient with future food and medicine applications. |
format |
Text |
author |
Yashu Chen Qin Mu Kai Hu Mo Chen Jifang Yang Jigang Chen Bijun Xie Zhida Sun |
author_facet |
Yashu Chen Qin Mu Kai Hu Mo Chen Jifang Yang Jigang Chen Bijun Xie Zhida Sun |
author_sort |
Yashu Chen |
title |
Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements |
title_short |
Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements |
title_full |
Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements |
title_fullStr |
Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements |
title_full_unstemmed |
Characterization of MK8(H2) from Rhodococcus sp. B7740 and Its Potential Antiglycation Capacity Measurements |
title_sort |
characterization of mk8(h2) from rhodococcus sp. b7740 and its potential antiglycation capacity measurements |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2018 |
url |
https://doi.org/10.3390/md16100391 |
op_coverage |
agris |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean |
genre_facet |
Arctic Arctic Ocean |
op_source |
Marine Drugs; Volume 16; Issue 10; Pages: 391 |
op_relation |
https://dx.doi.org/10.3390/md16100391 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/md16100391 |
container_title |
Marine Drugs |
container_volume |
16 |
container_issue |
10 |
container_start_page |
391 |
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