Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland
Twenty-eight sponge specimens were collected at a shallow water hydrothermal vent site north of Iceland. Extracts were prepared and tested in vitro for cytotoxic activity, and eight of them were shown to be cytotoxic. A mass spectrometry (MS)-based metabolomics approach was used to determine the che...
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ftmdpi:oai:mdpi.com:/1660-3397/15/2/52/ 2023-08-20T04:07:24+02:00 Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland Eydis Einarsdottir Manuela Magnusdottir Giuseppe Astarita Matthias Köck Helga Ögmundsdottir Margret Thorsteinsdottir Hans Rapp Sesselja Omarsdottir Giuseppe Paglia agris 2017-02-22 application/pdf https://doi.org/10.3390/md15020052 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md15020052 https://creativecommons.org/licenses/by/4.0/ Marine Drugs; Volume 15; Issue 2; Pages: 52 marine natural products sponges metabolomics ion mobility Haliclona rosea Text 2017 ftmdpi https://doi.org/10.3390/md15020052 2023-07-31T21:03:23Z Twenty-eight sponge specimens were collected at a shallow water hydrothermal vent site north of Iceland. Extracts were prepared and tested in vitro for cytotoxic activity, and eight of them were shown to be cytotoxic. A mass spectrometry (MS)-based metabolomics approach was used to determine the chemical composition of the extracts. This analysis highlighted clear differences in the metabolomes of three sponge specimens, and all of them were identified as Haliclona (Rhizoniera) rosea (Bowerbank, 1866). Therefore, these specimens were selected for further investigation. Haliclona rosea metabolomes contained a class of potential key compounds, the 3-alkyl pyridine alkaloids (3-APA) responsible for the cytotoxic activity of the fractions. Several 3-APA compounds were tentatively identified including haliclamines, cyclostellettamines, viscosalines and viscosamines. Among these compounds, cyclostellettamine P was tentatively identified for the first time by using ion mobility MS in time-aligned parallel (TAP) fragmentation mode. In this work, we show the potential of applying metabolomics strategies and in particular the utility of coupling ion mobility with MS for the molecular characterization of sponge specimens. Text Iceland MDPI Open Access Publishing Marine Drugs 15 2 52 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
marine natural products sponges metabolomics ion mobility Haliclona rosea |
spellingShingle |
marine natural products sponges metabolomics ion mobility Haliclona rosea Eydis Einarsdottir Manuela Magnusdottir Giuseppe Astarita Matthias Köck Helga Ögmundsdottir Margret Thorsteinsdottir Hans Rapp Sesselja Omarsdottir Giuseppe Paglia Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
topic_facet |
marine natural products sponges metabolomics ion mobility Haliclona rosea |
description |
Twenty-eight sponge specimens were collected at a shallow water hydrothermal vent site north of Iceland. Extracts were prepared and tested in vitro for cytotoxic activity, and eight of them were shown to be cytotoxic. A mass spectrometry (MS)-based metabolomics approach was used to determine the chemical composition of the extracts. This analysis highlighted clear differences in the metabolomes of three sponge specimens, and all of them were identified as Haliclona (Rhizoniera) rosea (Bowerbank, 1866). Therefore, these specimens were selected for further investigation. Haliclona rosea metabolomes contained a class of potential key compounds, the 3-alkyl pyridine alkaloids (3-APA) responsible for the cytotoxic activity of the fractions. Several 3-APA compounds were tentatively identified including haliclamines, cyclostellettamines, viscosalines and viscosamines. Among these compounds, cyclostellettamine P was tentatively identified for the first time by using ion mobility MS in time-aligned parallel (TAP) fragmentation mode. In this work, we show the potential of applying metabolomics strategies and in particular the utility of coupling ion mobility with MS for the molecular characterization of sponge specimens. |
format |
Text |
author |
Eydis Einarsdottir Manuela Magnusdottir Giuseppe Astarita Matthias Köck Helga Ögmundsdottir Margret Thorsteinsdottir Hans Rapp Sesselja Omarsdottir Giuseppe Paglia |
author_facet |
Eydis Einarsdottir Manuela Magnusdottir Giuseppe Astarita Matthias Köck Helga Ögmundsdottir Margret Thorsteinsdottir Hans Rapp Sesselja Omarsdottir Giuseppe Paglia |
author_sort |
Eydis Einarsdottir |
title |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_short |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_full |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_fullStr |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_full_unstemmed |
Metabolic Profiling as a Screening Tool for Cytotoxic Compounds: Identification of 3-Alkyl Pyridine Alkaloids from Sponges Collected at a Shallow Water Hydrothermal Vent Site North of Iceland |
title_sort |
metabolic profiling as a screening tool for cytotoxic compounds: identification of 3-alkyl pyridine alkaloids from sponges collected at a shallow water hydrothermal vent site north of iceland |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2017 |
url |
https://doi.org/10.3390/md15020052 |
op_coverage |
agris |
genre |
Iceland |
genre_facet |
Iceland |
op_source |
Marine Drugs; Volume 15; Issue 2; Pages: 52 |
op_relation |
https://dx.doi.org/10.3390/md15020052 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.3390/md15020052 |
container_title |
Marine Drugs |
container_volume |
15 |
container_issue |
2 |
container_start_page |
52 |
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1774718994706595840 |