Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia)
We report on a novel arctic strain BM1 of a carotenogenic chlorophyte from a coastal habitat with harsh environmental conditions (wide variations in solar irradiance, temperature, salinity and nutrient availability) identified as Haematococcus pluvialis Flotow. Increased (25‰) salinity exerted no ad...
Published in: | Marine Drugs |
---|---|
Main Authors: | , , , , , |
Format: | Text |
Language: | English |
Published: |
Multidisciplinary Digital Publishing Institute
2014
|
Subjects: | |
Online Access: | https://doi.org/10.3390/md12084504 |
id |
ftmdpi:oai:mdpi.com:/1660-3397/12/8/4504/ |
---|---|
record_format |
openpolar |
spelling |
ftmdpi:oai:mdpi.com:/1660-3397/12/8/4504/ 2023-08-20T04:04:39+02:00 Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia) Konstantin Chekanov Elena Lobakova Irina Selyakh Larisa Semenova Roman Sidorov Alexei Solovchenko agris 2014-08-15 application/pdf https://doi.org/10.3390/md12084504 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md12084504 https://creativecommons.org/licenses/by/3.0/ Marine Drugs; Volume 12; Issue 8; Pages: 4504-4520 astaxanthin carotenogenesis fatty acids green microalgae Text 2014 ftmdpi https://doi.org/10.3390/md12084504 2023-07-31T20:38:48Z We report on a novel arctic strain BM1 of a carotenogenic chlorophyte from a coastal habitat with harsh environmental conditions (wide variations in solar irradiance, temperature, salinity and nutrient availability) identified as Haematococcus pluvialis Flotow. Increased (25‰) salinity exerted no adverse effect on the growth of the green BM1 cells. Under stressful conditions (high light, nitrogen and phosphorus deprivation), green vegetative cells of H. pluvialis BM1 grown in BG11 medium formed non-motile palmelloid cells and, eventually, hematocysts capable of a massive accumulation of the keto-carotenoid astaxanthin with a high nutraceutical and therapeutic potential. Routinely, astaxanthin was accumulated at the level of 4% of the cell dry weight (DW), reaching, under prolonged stress, 5.5% DW. Astaxanthin was predominantly accumulated in the form of mono- and diesters of fatty acids from C16 and C18 families. The palmelloids and hematocysts were characterized by the formation of red-colored cytoplasmic lipid droplets, increasingly large in size and number. The lipid droplets tended to merge and occupied almost the entire volume of the cell at the advanced stages of stress-induced carotenogenesis. The potential application of the new strain for the production of astaxanthin is discussed in comparison with the H. pluvialis strains currently employed in microalgal biotechnology. Text Arctic White Sea MDPI Open Access Publishing Arctic White Sea Marine Drugs 12 8 4504 4520 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
astaxanthin carotenogenesis fatty acids green microalgae |
spellingShingle |
astaxanthin carotenogenesis fatty acids green microalgae Konstantin Chekanov Elena Lobakova Irina Selyakh Larisa Semenova Roman Sidorov Alexei Solovchenko Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia) |
topic_facet |
astaxanthin carotenogenesis fatty acids green microalgae |
description |
We report on a novel arctic strain BM1 of a carotenogenic chlorophyte from a coastal habitat with harsh environmental conditions (wide variations in solar irradiance, temperature, salinity and nutrient availability) identified as Haematococcus pluvialis Flotow. Increased (25‰) salinity exerted no adverse effect on the growth of the green BM1 cells. Under stressful conditions (high light, nitrogen and phosphorus deprivation), green vegetative cells of H. pluvialis BM1 grown in BG11 medium formed non-motile palmelloid cells and, eventually, hematocysts capable of a massive accumulation of the keto-carotenoid astaxanthin with a high nutraceutical and therapeutic potential. Routinely, astaxanthin was accumulated at the level of 4% of the cell dry weight (DW), reaching, under prolonged stress, 5.5% DW. Astaxanthin was predominantly accumulated in the form of mono- and diesters of fatty acids from C16 and C18 families. The palmelloids and hematocysts were characterized by the formation of red-colored cytoplasmic lipid droplets, increasingly large in size and number. The lipid droplets tended to merge and occupied almost the entire volume of the cell at the advanced stages of stress-induced carotenogenesis. The potential application of the new strain for the production of astaxanthin is discussed in comparison with the H. pluvialis strains currently employed in microalgal biotechnology. |
format |
Text |
author |
Konstantin Chekanov Elena Lobakova Irina Selyakh Larisa Semenova Roman Sidorov Alexei Solovchenko |
author_facet |
Konstantin Chekanov Elena Lobakova Irina Selyakh Larisa Semenova Roman Sidorov Alexei Solovchenko |
author_sort |
Konstantin Chekanov |
title |
Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia) |
title_short |
Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia) |
title_full |
Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia) |
title_fullStr |
Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia) |
title_full_unstemmed |
Accumulation of Astaxanthin by a New Haematococcus pluvialis Strain BM1 from the White Sea Coastal Rocks (Russia) |
title_sort |
accumulation of astaxanthin by a new haematococcus pluvialis strain bm1 from the white sea coastal rocks (russia) |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2014 |
url |
https://doi.org/10.3390/md12084504 |
op_coverage |
agris |
geographic |
Arctic White Sea |
geographic_facet |
Arctic White Sea |
genre |
Arctic White Sea |
genre_facet |
Arctic White Sea |
op_source |
Marine Drugs; Volume 12; Issue 8; Pages: 4504-4520 |
op_relation |
https://dx.doi.org/10.3390/md12084504 |
op_rights |
https://creativecommons.org/licenses/by/3.0/ |
op_doi |
https://doi.org/10.3390/md12084504 |
container_title |
Marine Drugs |
container_volume |
12 |
container_issue |
8 |
container_start_page |
4504 |
op_container_end_page |
4520 |
_version_ |
1774715024985554944 |