Cytotoxic and Apoptosis-Inducing Activity of Triterpene Glycosides from Holothuria scabra and Cucumaria frondosa against HepG2 Cells
The cytotoxic effects of thirteen triterpene glycosides from Holothuria scabra Jaeger and Cucumaria frondosa Gunnerus (Holothuroidea) against four human cell lines were detected and their cytotoxicity-structure relationships were established. The apoptosis-inducing activity of a more potent glycosid...
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ftmdpi:oai:mdpi.com:/1660-3397/12/8/4274/ 2023-08-20T04:06:06+02:00 Cytotoxic and Apoptosis-Inducing Activity of Triterpene Glycosides from Holothuria scabra and Cucumaria frondosa against HepG2 Cells Juanjuan Wang Hua Han Xiangfeng Chen Yanghua Yi Hongxiang Sun agris 2014-07-24 application/pdf https://doi.org/10.3390/md12084274 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md12084274 https://creativecommons.org/licenses/by/3.0/ Marine Drugs; Volume 12; Issue 8; Pages: 4274-4290 Holothuria scabra Cucumaria frondosa triterpene glycosides echinoside A cytotoxicity structure-activity relationship apoptosis mechanism Text 2014 ftmdpi https://doi.org/10.3390/md12084274 2023-07-31T20:38:28Z The cytotoxic effects of thirteen triterpene glycosides from Holothuria scabra Jaeger and Cucumaria frondosa Gunnerus (Holothuroidea) against four human cell lines were detected and their cytotoxicity-structure relationships were established. The apoptosis-inducing activity of a more potent glycoside echinoside A (1) in HepG2 cells was further investigated by determining its effect on the morphology, mitochondrial transmembrane potential (Δψm) and mRNA expression levels of the apoptosis-related genes. The results showed that the number of glycosyl residues in sugar chains and the side chain of aglycone could affect their cytotoxicity towards tumor cells and selective cytotoxicity. 1 significantly inhibited cell viability and induced apoptosis in HepG2 cells. 1 also markedly decreased the Δψm and Bcl-2/Bax mRNA express ratio, and up-regulated the mRNA expression levels of Caspase-3, Caspase-8 and Caspase-9 in HepG2 cells. Therefore, 1 induced apoptosis in HepG2 cells through both intrinsic and extrinsic pathway. These findings could potentially promote the usage of these glycosides as leading compounds for developing new antitumor drugs. Text Cucumaria frondosa MDPI Open Access Publishing Marine Drugs 12 8 4274 4290 |
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Open Polar |
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MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
Holothuria scabra Cucumaria frondosa triterpene glycosides echinoside A cytotoxicity structure-activity relationship apoptosis mechanism |
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Holothuria scabra Cucumaria frondosa triterpene glycosides echinoside A cytotoxicity structure-activity relationship apoptosis mechanism Juanjuan Wang Hua Han Xiangfeng Chen Yanghua Yi Hongxiang Sun Cytotoxic and Apoptosis-Inducing Activity of Triterpene Glycosides from Holothuria scabra and Cucumaria frondosa against HepG2 Cells |
topic_facet |
Holothuria scabra Cucumaria frondosa triterpene glycosides echinoside A cytotoxicity structure-activity relationship apoptosis mechanism |
description |
The cytotoxic effects of thirteen triterpene glycosides from Holothuria scabra Jaeger and Cucumaria frondosa Gunnerus (Holothuroidea) against four human cell lines were detected and their cytotoxicity-structure relationships were established. The apoptosis-inducing activity of a more potent glycoside echinoside A (1) in HepG2 cells was further investigated by determining its effect on the morphology, mitochondrial transmembrane potential (Δψm) and mRNA expression levels of the apoptosis-related genes. The results showed that the number of glycosyl residues in sugar chains and the side chain of aglycone could affect their cytotoxicity towards tumor cells and selective cytotoxicity. 1 significantly inhibited cell viability and induced apoptosis in HepG2 cells. 1 also markedly decreased the Δψm and Bcl-2/Bax mRNA express ratio, and up-regulated the mRNA expression levels of Caspase-3, Caspase-8 and Caspase-9 in HepG2 cells. Therefore, 1 induced apoptosis in HepG2 cells through both intrinsic and extrinsic pathway. These findings could potentially promote the usage of these glycosides as leading compounds for developing new antitumor drugs. |
format |
Text |
author |
Juanjuan Wang Hua Han Xiangfeng Chen Yanghua Yi Hongxiang Sun |
author_facet |
Juanjuan Wang Hua Han Xiangfeng Chen Yanghua Yi Hongxiang Sun |
author_sort |
Juanjuan Wang |
title |
Cytotoxic and Apoptosis-Inducing Activity of Triterpene Glycosides from Holothuria scabra and Cucumaria frondosa against HepG2 Cells |
title_short |
Cytotoxic and Apoptosis-Inducing Activity of Triterpene Glycosides from Holothuria scabra and Cucumaria frondosa against HepG2 Cells |
title_full |
Cytotoxic and Apoptosis-Inducing Activity of Triterpene Glycosides from Holothuria scabra and Cucumaria frondosa against HepG2 Cells |
title_fullStr |
Cytotoxic and Apoptosis-Inducing Activity of Triterpene Glycosides from Holothuria scabra and Cucumaria frondosa against HepG2 Cells |
title_full_unstemmed |
Cytotoxic and Apoptosis-Inducing Activity of Triterpene Glycosides from Holothuria scabra and Cucumaria frondosa against HepG2 Cells |
title_sort |
cytotoxic and apoptosis-inducing activity of triterpene glycosides from holothuria scabra and cucumaria frondosa against hepg2 cells |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2014 |
url |
https://doi.org/10.3390/md12084274 |
op_coverage |
agris |
genre |
Cucumaria frondosa |
genre_facet |
Cucumaria frondosa |
op_source |
Marine Drugs; Volume 12; Issue 8; Pages: 4274-4290 |
op_relation |
https://dx.doi.org/10.3390/md12084274 |
op_rights |
https://creativecommons.org/licenses/by/3.0/ |
op_doi |
https://doi.org/10.3390/md12084274 |
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Marine Drugs |
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12 |
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8 |
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4274 |
op_container_end_page |
4290 |
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1774717018123010048 |