Phylogenetic Diversity and Biological Activity of Actinobacteria Isolated from the Chukchi Shelf Marine Sediments in the Arctic Ocean
Marine environments are a rich source of Actinobacteria and have the potential to produce a wide variety of biologically active secondary metabolites. In this study, we used four selective isolation media to culture Actinobacteria from the sediments collected from the Chukchi Shelf in the Arctic Oce...
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ftmdpi:oai:mdpi.com:/1660-3397/12/3/1281/ 2023-08-20T04:04:05+02:00 Phylogenetic Diversity and Biological Activity of Actinobacteria Isolated from the Chukchi Shelf Marine Sediments in the Arctic Ocean Meng Yuan Yong Yu Hui-Rong Li Ning Dong Xiao-Hua Zhang agris 2014-03-06 application/pdf https://doi.org/10.3390/md12031281 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md12031281 https://creativecommons.org/licenses/by/3.0/ Marine Drugs; Volume 12; Issue 3; Pages: 1281-1297 marine Actinobacteria phylogenetic diversity biological activity Arctic Ocean Text 2014 ftmdpi https://doi.org/10.3390/md12031281 2023-07-31T20:36:15Z Marine environments are a rich source of Actinobacteria and have the potential to produce a wide variety of biologically active secondary metabolites. In this study, we used four selective isolation media to culture Actinobacteria from the sediments collected from the Chukchi Shelf in the Arctic Ocean. A total of 73 actinobacterial strains were isolated. Based on repetitive DNA fingerprinting analysis, we selected 30 representatives for partial characterization according to their phylogenetic diversity, antimicrobial activities and secondary-metabolite biosynthesis genes. Results from the 16S rRNA gene sequence analysis indicated that the 30 strains could be sorted into 18 phylotypes belonging to 14 different genera: Agrococcus, Arsenicicoccus, Arthrobacter, Brevibacterium, Citricoccus, Janibacter, Kocuria, Microbacterium, Microlunatus, Nocardioides, Nocardiopsis, Saccharopolyspora, Salinibacterium and Streptomyces. To our knowledge, this paper is the first report on the isolation of Microlunatus genus members from marine habitats. Of the 30 isolates, 11 strains exhibited antibacterial and/or antifungal activity, seven of which have activities against Bacillus subtilis and Candida albicans. All 30 strains have at least two biosynthetic genes, one-third of which possess more than four biosynthetic genes. This study demonstrates the significant diversity of Actinobacteria in the Chukchi Shelf sediment and their potential for producing biologically active compounds and novel material for genetic manipulation or combinatorial biosynthesis. Text Arctic Arctic Ocean Chukchi MDPI Open Access Publishing Arctic Arctic Ocean Chukchi Shelf ENVELOPE(-169.167,-169.167,70.550,70.550) Marine Drugs 12 3 1281 1297 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
marine Actinobacteria phylogenetic diversity biological activity Arctic Ocean |
spellingShingle |
marine Actinobacteria phylogenetic diversity biological activity Arctic Ocean Meng Yuan Yong Yu Hui-Rong Li Ning Dong Xiao-Hua Zhang Phylogenetic Diversity and Biological Activity of Actinobacteria Isolated from the Chukchi Shelf Marine Sediments in the Arctic Ocean |
topic_facet |
marine Actinobacteria phylogenetic diversity biological activity Arctic Ocean |
description |
Marine environments are a rich source of Actinobacteria and have the potential to produce a wide variety of biologically active secondary metabolites. In this study, we used four selective isolation media to culture Actinobacteria from the sediments collected from the Chukchi Shelf in the Arctic Ocean. A total of 73 actinobacterial strains were isolated. Based on repetitive DNA fingerprinting analysis, we selected 30 representatives for partial characterization according to their phylogenetic diversity, antimicrobial activities and secondary-metabolite biosynthesis genes. Results from the 16S rRNA gene sequence analysis indicated that the 30 strains could be sorted into 18 phylotypes belonging to 14 different genera: Agrococcus, Arsenicicoccus, Arthrobacter, Brevibacterium, Citricoccus, Janibacter, Kocuria, Microbacterium, Microlunatus, Nocardioides, Nocardiopsis, Saccharopolyspora, Salinibacterium and Streptomyces. To our knowledge, this paper is the first report on the isolation of Microlunatus genus members from marine habitats. Of the 30 isolates, 11 strains exhibited antibacterial and/or antifungal activity, seven of which have activities against Bacillus subtilis and Candida albicans. All 30 strains have at least two biosynthetic genes, one-third of which possess more than four biosynthetic genes. This study demonstrates the significant diversity of Actinobacteria in the Chukchi Shelf sediment and their potential for producing biologically active compounds and novel material for genetic manipulation or combinatorial biosynthesis. |
format |
Text |
author |
Meng Yuan Yong Yu Hui-Rong Li Ning Dong Xiao-Hua Zhang |
author_facet |
Meng Yuan Yong Yu Hui-Rong Li Ning Dong Xiao-Hua Zhang |
author_sort |
Meng Yuan |
title |
Phylogenetic Diversity and Biological Activity of Actinobacteria Isolated from the Chukchi Shelf Marine Sediments in the Arctic Ocean |
title_short |
Phylogenetic Diversity and Biological Activity of Actinobacteria Isolated from the Chukchi Shelf Marine Sediments in the Arctic Ocean |
title_full |
Phylogenetic Diversity and Biological Activity of Actinobacteria Isolated from the Chukchi Shelf Marine Sediments in the Arctic Ocean |
title_fullStr |
Phylogenetic Diversity and Biological Activity of Actinobacteria Isolated from the Chukchi Shelf Marine Sediments in the Arctic Ocean |
title_full_unstemmed |
Phylogenetic Diversity and Biological Activity of Actinobacteria Isolated from the Chukchi Shelf Marine Sediments in the Arctic Ocean |
title_sort |
phylogenetic diversity and biological activity of actinobacteria isolated from the chukchi shelf marine sediments in the arctic ocean |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2014 |
url |
https://doi.org/10.3390/md12031281 |
op_coverage |
agris |
long_lat |
ENVELOPE(-169.167,-169.167,70.550,70.550) |
geographic |
Arctic Arctic Ocean Chukchi Shelf |
geographic_facet |
Arctic Arctic Ocean Chukchi Shelf |
genre |
Arctic Arctic Ocean Chukchi |
genre_facet |
Arctic Arctic Ocean Chukchi |
op_source |
Marine Drugs; Volume 12; Issue 3; Pages: 1281-1297 |
op_relation |
https://dx.doi.org/10.3390/md12031281 |
op_rights |
https://creativecommons.org/licenses/by/3.0/ |
op_doi |
https://doi.org/10.3390/md12031281 |
container_title |
Marine Drugs |
container_volume |
12 |
container_issue |
3 |
container_start_page |
1281 |
op_container_end_page |
1297 |
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1774714499916365824 |