Isolation and Characterization of Anti-Adenoviral Secondary Metabolites from Marine Actinobacteria
Adenovirus infections in immunocompromised patients are associated with high mortality rates. Currently, there are no effective anti-adenoviral therapies available. It is well known that actinobacteria can produce secondary metabolites that are attractive in drug discovery due to their structural di...
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ftmdpi:oai:mdpi.com:/1660-3397/12/2/799/ 2023-08-20T04:10:18+02:00 Isolation and Characterization of Anti-Adenoviral Secondary Metabolites from Marine Actinobacteria Mårten Strand Marcus Carlsson Hanna Uvell Koushikul Islam Karin Edlund Inger Cullman Björn Altermark Ya-Fang Mei Mikael Elofsson Nils-Peder Willassen Göran Wadell Fredrik Almqvist agris 2014-01-28 application/pdf https://doi.org/10.3390/md12020799 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md12020799 https://creativecommons.org/licenses/by/3.0/ Marine Drugs; Volume 12; Issue 2; Pages: 799-821 adenovirus antiviral natural products secondary metabolites marine actinobacteria extract screening butenolides Text 2014 ftmdpi https://doi.org/10.3390/md12020799 2023-07-31T20:35:43Z Adenovirus infections in immunocompromised patients are associated with high mortality rates. Currently, there are no effective anti-adenoviral therapies available. It is well known that actinobacteria can produce secondary metabolites that are attractive in drug discovery due to their structural diversity and their evolved interaction with biomolecules. Here, we have established an extract library derived from actinobacteria isolated from Vestfjorden, Norway, and performed a screening campaign to discover anti-adenoviral compounds. One extract with anti-adenoviral activity was found to contain a diastereomeric 1:1 mixture of the butenolide secondary alcohols 1a and 1b. By further cultivation and analysis, we could isolate 1a and 1b in different diastereomeric ratio. In addition, three more anti-adenoviral butenolides 2, 3 and 4 with differences in their side-chains were isolated. In this study, the anti-adenoviral activity of these compounds was characterized and substantial differences in the cytotoxic potential between the butenolide analogs were observed. The most potent butenolide analog 3 displayed an EC50 value of 91 μM and no prominent cytotoxicity at 2 mM. Furthermore, we propose a biosynthetic pathway for these compounds based on their relative time of appearance and structure. Text Vestfjorden MDPI Open Access Publishing Norway Marine Drugs 12 2 799 821 |
institution |
Open Polar |
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MDPI Open Access Publishing |
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ftmdpi |
language |
English |
topic |
adenovirus antiviral natural products secondary metabolites marine actinobacteria extract screening butenolides |
spellingShingle |
adenovirus antiviral natural products secondary metabolites marine actinobacteria extract screening butenolides Mårten Strand Marcus Carlsson Hanna Uvell Koushikul Islam Karin Edlund Inger Cullman Björn Altermark Ya-Fang Mei Mikael Elofsson Nils-Peder Willassen Göran Wadell Fredrik Almqvist Isolation and Characterization of Anti-Adenoviral Secondary Metabolites from Marine Actinobacteria |
topic_facet |
adenovirus antiviral natural products secondary metabolites marine actinobacteria extract screening butenolides |
description |
Adenovirus infections in immunocompromised patients are associated with high mortality rates. Currently, there are no effective anti-adenoviral therapies available. It is well known that actinobacteria can produce secondary metabolites that are attractive in drug discovery due to their structural diversity and their evolved interaction with biomolecules. Here, we have established an extract library derived from actinobacteria isolated from Vestfjorden, Norway, and performed a screening campaign to discover anti-adenoviral compounds. One extract with anti-adenoviral activity was found to contain a diastereomeric 1:1 mixture of the butenolide secondary alcohols 1a and 1b. By further cultivation and analysis, we could isolate 1a and 1b in different diastereomeric ratio. In addition, three more anti-adenoviral butenolides 2, 3 and 4 with differences in their side-chains were isolated. In this study, the anti-adenoviral activity of these compounds was characterized and substantial differences in the cytotoxic potential between the butenolide analogs were observed. The most potent butenolide analog 3 displayed an EC50 value of 91 μM and no prominent cytotoxicity at 2 mM. Furthermore, we propose a biosynthetic pathway for these compounds based on their relative time of appearance and structure. |
format |
Text |
author |
Mårten Strand Marcus Carlsson Hanna Uvell Koushikul Islam Karin Edlund Inger Cullman Björn Altermark Ya-Fang Mei Mikael Elofsson Nils-Peder Willassen Göran Wadell Fredrik Almqvist |
author_facet |
Mårten Strand Marcus Carlsson Hanna Uvell Koushikul Islam Karin Edlund Inger Cullman Björn Altermark Ya-Fang Mei Mikael Elofsson Nils-Peder Willassen Göran Wadell Fredrik Almqvist |
author_sort |
Mårten Strand |
title |
Isolation and Characterization of Anti-Adenoviral Secondary Metabolites from Marine Actinobacteria |
title_short |
Isolation and Characterization of Anti-Adenoviral Secondary Metabolites from Marine Actinobacteria |
title_full |
Isolation and Characterization of Anti-Adenoviral Secondary Metabolites from Marine Actinobacteria |
title_fullStr |
Isolation and Characterization of Anti-Adenoviral Secondary Metabolites from Marine Actinobacteria |
title_full_unstemmed |
Isolation and Characterization of Anti-Adenoviral Secondary Metabolites from Marine Actinobacteria |
title_sort |
isolation and characterization of anti-adenoviral secondary metabolites from marine actinobacteria |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2014 |
url |
https://doi.org/10.3390/md12020799 |
op_coverage |
agris |
geographic |
Norway |
geographic_facet |
Norway |
genre |
Vestfjorden |
genre_facet |
Vestfjorden |
op_source |
Marine Drugs; Volume 12; Issue 2; Pages: 799-821 |
op_relation |
https://dx.doi.org/10.3390/md12020799 |
op_rights |
https://creativecommons.org/licenses/by/3.0/ |
op_doi |
https://doi.org/10.3390/md12020799 |
container_title |
Marine Drugs |
container_volume |
12 |
container_issue |
2 |
container_start_page |
799 |
op_container_end_page |
821 |
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