In Vitro Acylation of Okadaic Acid in the Presence of Various Bivalves’ Extracts
The dinoflagellate Dinophysis spp. is responsible for diarrhetic shellfish poisoning (DSP). In the bivalves exposed to the toxic bloom of the dinoflagellate, dinophysistoxin 3 (DTX3), the 7-OH acylated form of either okadaic acid (OA) or DTX1, is produced. We demonstrated in vitro acylation of OA wi...
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ftmdpi:oai:mdpi.com:/1660-3397/11/2/300/ 2023-08-20T04:06:04+02:00 In Vitro Acylation of Okadaic Acid in the Presence of Various Bivalves’ Extracts Keiichi Konoki Tatsuya Onoda Ryuichi Watanabe Yuko Cho Shinnosuke Kaga Toshiyuki Suzuki Mari Yotsu-Yamashita agris 2013-01-29 application/pdf https://doi.org/10.3390/md11020300 EN eng Multidisciplinary Digital Publishing Institute https://dx.doi.org/10.3390/md11020300 https://creativecommons.org/licenses/by/3.0/ Marine Drugs; Volume 11; Issue 2; Pages: 300-315 okadaic acid dinophysistoxin Halichondria okadai okadaic acid binding protein diarrhetic shellfish poisoning acyl coenzyme A transferase detoxification Text 2013 ftmdpi https://doi.org/10.3390/md11020300 2023-07-31T20:31:26Z The dinoflagellate Dinophysis spp. is responsible for diarrhetic shellfish poisoning (DSP). In the bivalves exposed to the toxic bloom of the dinoflagellate, dinophysistoxin 3 (DTX3), the 7-OH acylated form of either okadaic acid (OA) or DTX1, is produced. We demonstrated in vitro acylation of OA with palmitoyl CoA in the presence of protein extract from the digestive gland, but not other tissues of the bivalve Mizuhopecten yessoensis. The yield of 7-O-palmitoyl OA reached its maximum within 2 h, was the highest at 37 °C followed by 28 °C, 16 °C and 4 °C and was the highest at pH 8 in comparison with the yields at pH 6 and pH 4. The transformation also proceeded when the protein extract was prepared from the bivalves Corbicula japonica and Crassostrea gigas. The OA binding protein OABP2 identified in the sponge Halichondria okadai was not detected in the bivalve M. yessoensis, the bivalve Mytilus galloprovincialis and the ascidian Halocynthia roretzi, though they are known to accumulate diarrhetic shellfish poisoning toxins. Since DTX3 does not bind to protein phosphatases 1 and 2A, the physiological target for OA and DTXs in mammalian cells, the acylation of DSP toxins would be related to a detoxification mechanism for the bivalve species. Text Crassostrea gigas MDPI Open Access Publishing Marine Drugs 11 12 300 315 |
institution |
Open Polar |
collection |
MDPI Open Access Publishing |
op_collection_id |
ftmdpi |
language |
English |
topic |
okadaic acid dinophysistoxin Halichondria okadai okadaic acid binding protein diarrhetic shellfish poisoning acyl coenzyme A transferase detoxification |
spellingShingle |
okadaic acid dinophysistoxin Halichondria okadai okadaic acid binding protein diarrhetic shellfish poisoning acyl coenzyme A transferase detoxification Keiichi Konoki Tatsuya Onoda Ryuichi Watanabe Yuko Cho Shinnosuke Kaga Toshiyuki Suzuki Mari Yotsu-Yamashita In Vitro Acylation of Okadaic Acid in the Presence of Various Bivalves’ Extracts |
topic_facet |
okadaic acid dinophysistoxin Halichondria okadai okadaic acid binding protein diarrhetic shellfish poisoning acyl coenzyme A transferase detoxification |
description |
The dinoflagellate Dinophysis spp. is responsible for diarrhetic shellfish poisoning (DSP). In the bivalves exposed to the toxic bloom of the dinoflagellate, dinophysistoxin 3 (DTX3), the 7-OH acylated form of either okadaic acid (OA) or DTX1, is produced. We demonstrated in vitro acylation of OA with palmitoyl CoA in the presence of protein extract from the digestive gland, but not other tissues of the bivalve Mizuhopecten yessoensis. The yield of 7-O-palmitoyl OA reached its maximum within 2 h, was the highest at 37 °C followed by 28 °C, 16 °C and 4 °C and was the highest at pH 8 in comparison with the yields at pH 6 and pH 4. The transformation also proceeded when the protein extract was prepared from the bivalves Corbicula japonica and Crassostrea gigas. The OA binding protein OABP2 identified in the sponge Halichondria okadai was not detected in the bivalve M. yessoensis, the bivalve Mytilus galloprovincialis and the ascidian Halocynthia roretzi, though they are known to accumulate diarrhetic shellfish poisoning toxins. Since DTX3 does not bind to protein phosphatases 1 and 2A, the physiological target for OA and DTXs in mammalian cells, the acylation of DSP toxins would be related to a detoxification mechanism for the bivalve species. |
format |
Text |
author |
Keiichi Konoki Tatsuya Onoda Ryuichi Watanabe Yuko Cho Shinnosuke Kaga Toshiyuki Suzuki Mari Yotsu-Yamashita |
author_facet |
Keiichi Konoki Tatsuya Onoda Ryuichi Watanabe Yuko Cho Shinnosuke Kaga Toshiyuki Suzuki Mari Yotsu-Yamashita |
author_sort |
Keiichi Konoki |
title |
In Vitro Acylation of Okadaic Acid in the Presence of Various Bivalves’ Extracts |
title_short |
In Vitro Acylation of Okadaic Acid in the Presence of Various Bivalves’ Extracts |
title_full |
In Vitro Acylation of Okadaic Acid in the Presence of Various Bivalves’ Extracts |
title_fullStr |
In Vitro Acylation of Okadaic Acid in the Presence of Various Bivalves’ Extracts |
title_full_unstemmed |
In Vitro Acylation of Okadaic Acid in the Presence of Various Bivalves’ Extracts |
title_sort |
in vitro acylation of okadaic acid in the presence of various bivalves’ extracts |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2013 |
url |
https://doi.org/10.3390/md11020300 |
op_coverage |
agris |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Marine Drugs; Volume 11; Issue 2; Pages: 300-315 |
op_relation |
https://dx.doi.org/10.3390/md11020300 |
op_rights |
https://creativecommons.org/licenses/by/3.0/ |
op_doi |
https://doi.org/10.3390/md11020300 |
container_title |
Marine Drugs |
container_volume |
11 |
container_issue |
12 |
container_start_page |
300 |
op_container_end_page |
315 |
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1774716987315847168 |