Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2)
Hemocytes mediate a series of immune reactions essential for bivalve survival in the environment, however, the impact of harmful algal species and their associated phycotoxins upon bivalve immune system is under debate. To better understand the possible toxic effects of these toxins, Crassostrea gig...
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Molecular Diversity Preservation International
2012
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ftmdpi:oai:mdpi.com:/1660-3397/10/3/583/ 2023-08-20T04:06:01+02:00 Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) Danielle F. Mello Eliza S. De Oliveira Renato C. Vieira Erik Simoes Rafael Trevisan Alcir Luiz Dafre Margherita Anna Barracco agris 2012-03-05 application/pdf https://doi.org/10.3390/md10030583 EN eng Molecular Diversity Preservation International https://dx.doi.org/10.3390/md10030583 https://creativecommons.org/licenses/by/3.0/ Marine Drugs; Volume 10; Issue 3; Pages: 583-597 brevetoxin hemocytes bivalves gene expression immune antioxidant and detoxification systems Text 2012 ftmdpi https://doi.org/10.3390/md10030583 2023-07-31T20:28:22Z Hemocytes mediate a series of immune reactions essential for bivalve survival in the environment, however, the impact of harmful algal species and their associated phycotoxins upon bivalve immune system is under debate. To better understand the possible toxic effects of these toxins, Crassostrea gigas hemocytes were exposed to brevetoxin (PbTx-2). Hemocyte viability, monitored through the neutral red retention and MTT reduction assays, and apoptosis (Hoechst staining) remained unchanged during 12 h of exposure to PbTx-2 in concentrations up to 1000 µg/L. Despite cell viability and apoptosis remained stable, hemocytes incubated for 4 h with 1000 µg/L of PbTx-2 revealed higher expression levels of Hsp70 (p < 0.01) and CYP356A1 ( p < 0.05) transcripts and a tendency to increase FABP expression, as evaluated by Real-Time quantitative PCR. The expression of other studied genes (BPI, IL-17, GSTO, EcSOD, Prx6, SOD and GPx) remained unchanged. The results suggest that the absence of cytotoxic effects of PbTx-2 in Crassostrea gigas hemocytes, even at high concentrations, allow early defense responses to be produced by activating protective mechanisms associated to detoxification (CYP356A1 and possibly FABP) and stress (Hsp70), but not to immune or to antioxidant (BPI, IL-17, EcSOD, Prx6, GPx and SOD) related genes. Text Crassostrea gigas MDPI Open Access Publishing Marine Drugs 10 12 583 597 |
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ftmdpi |
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English |
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brevetoxin hemocytes bivalves gene expression immune antioxidant and detoxification systems |
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brevetoxin hemocytes bivalves gene expression immune antioxidant and detoxification systems Danielle F. Mello Eliza S. De Oliveira Renato C. Vieira Erik Simoes Rafael Trevisan Alcir Luiz Dafre Margherita Anna Barracco Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) |
topic_facet |
brevetoxin hemocytes bivalves gene expression immune antioxidant and detoxification systems |
description |
Hemocytes mediate a series of immune reactions essential for bivalve survival in the environment, however, the impact of harmful algal species and their associated phycotoxins upon bivalve immune system is under debate. To better understand the possible toxic effects of these toxins, Crassostrea gigas hemocytes were exposed to brevetoxin (PbTx-2). Hemocyte viability, monitored through the neutral red retention and MTT reduction assays, and apoptosis (Hoechst staining) remained unchanged during 12 h of exposure to PbTx-2 in concentrations up to 1000 µg/L. Despite cell viability and apoptosis remained stable, hemocytes incubated for 4 h with 1000 µg/L of PbTx-2 revealed higher expression levels of Hsp70 (p < 0.01) and CYP356A1 ( p < 0.05) transcripts and a tendency to increase FABP expression, as evaluated by Real-Time quantitative PCR. The expression of other studied genes (BPI, IL-17, GSTO, EcSOD, Prx6, SOD and GPx) remained unchanged. The results suggest that the absence of cytotoxic effects of PbTx-2 in Crassostrea gigas hemocytes, even at high concentrations, allow early defense responses to be produced by activating protective mechanisms associated to detoxification (CYP356A1 and possibly FABP) and stress (Hsp70), but not to immune or to antioxidant (BPI, IL-17, EcSOD, Prx6, GPx and SOD) related genes. |
format |
Text |
author |
Danielle F. Mello Eliza S. De Oliveira Renato C. Vieira Erik Simoes Rafael Trevisan Alcir Luiz Dafre Margherita Anna Barracco |
author_facet |
Danielle F. Mello Eliza S. De Oliveira Renato C. Vieira Erik Simoes Rafael Trevisan Alcir Luiz Dafre Margherita Anna Barracco |
author_sort |
Danielle F. Mello |
title |
Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) |
title_short |
Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) |
title_full |
Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) |
title_fullStr |
Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) |
title_full_unstemmed |
Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) |
title_sort |
cellular and transcriptional responses of crassostrea gigas hemocytes exposed in vitro to brevetoxin (pbtx-2) |
publisher |
Molecular Diversity Preservation International |
publishDate |
2012 |
url |
https://doi.org/10.3390/md10030583 |
op_coverage |
agris |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Marine Drugs; Volume 10; Issue 3; Pages: 583-597 |
op_relation |
https://dx.doi.org/10.3390/md10030583 |
op_rights |
https://creativecommons.org/licenses/by/3.0/ |
op_doi |
https://doi.org/10.3390/md10030583 |
container_title |
Marine Drugs |
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10 |
container_issue |
12 |
container_start_page |
583 |
op_container_end_page |
597 |
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